DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR IRBESARTAN AND ATORVASTATIN BY SIMULTANEOUS EQUTION SPECTROSCOPIC METHOD
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Irbesartan and atorvastatin in synthetic mixture using simultaneous eqution Method. In this spectroscopicÂ method, 226.00 nm and 246.00 nm wavelengthsÂ were selected for measurement of absorptivity. Both the drugs show linearity in a concentration range of 05-30 Î¼g/ml at their respective Î»max. Accuracy, precision and recovery studies were done by QC samples covering lower, medium and high concentrations of the linearity range. The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (<2%). The limit of determination was 0.033Î¼g/ml and 0.125 Î¼g/ml for Irbesartan and atorvastatin, respectively. The limit of quantification was 0.1008 Î¼g/ml and 0.3792 Î¼g/ml for Irbesartan and atorvastatin, respectively. Recovery of Irbesartan and atorvastatin were found to be 99.75 % and 99.52% respectively confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.
Asif H, Sabir AM and Parminder SB. A review of pharmacological and pharmaceutical profile of Irbesartan. Pharmacophore. 2(6); 2011: 276-86.
Irbesartan drug info in drugbank. (database available on internet): http://www.drugbank.ca/drugs/db01029
Irbesartan drug info. (database available on internet): http://en.wikipedia.org/wiki/irbesartan
Dileep N, Siva P, Santhi K and Sajeeth C. A review on atorvastatin co administration with ezetimibe for the treatment of hypercholesterolemia. Int J Pharm Chemica Sci. 1(2); 2012: 756-60.
Atorvastatin drug info in drugbank. (database available on internet): http://www.drugbank.ca/drugs/db01076
Virani P, Sojitra R, Raj H and Jain V. A review on Irbesartan co administered with Atorvastatin for the treatment of cardiac risk. J Crit Rev. 1(1); 2014: 25-28.
Antonio C, Roberta A, Roberto D. et al. Effect of atorvastatin and Irbesartan, alone and in combination, on postprandial endothelial dysfunction, oxidative stress, and inflammation in type 2 diabetic patients. Circulation-American Heart Association. 111; 2013: 2517-24.
Virani P, Sojitra R, Raj H and Jain V. Irbesartan: A review on analytical method and its determination in pharmaceuticals and biological matrix. Inventi Rapid: Pharm Analysis & Quality Assurance. 4; 2014: 1-6.
Virani P, Sojitra R, Raj H and Jain V. Atorvastatin: A review on analytical method and its determination in pharmaceuticals and biological matrix. Inventi Rapid: Pharm Analysis & Quality Assurance. 4; 2014: 1-6.
Virani P, Raj H, Jain V and Jain P. Updated review: validation and method validation parameters. Pharmatutor. 2(10); 2014: 27-37.