• Midhun Kumar Duddu Pharmaceutical Biotechnology Division, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam (A.P.), India, 530003
  • Girijasankar Guntuku Pharmaceutical Biotechnology Division, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam (A.P.), India, 530003



Actinomycetes, Cross-streak method, Agar well diffusion method, Crude extracts, Antibiotics, Antibacterial activity


Objective: To isolate, screen and characterize antibiotic producing actinomycetes from Kapuluppada plastic waste dumping yard, Visakhapatnam.

Methods: A total of 12 soil samples were collected, serially diluted and spread on starch casein agar supplemented with Rifampicin and Cycloheximide for inhibition of bacteria and fungi, respectively. Cross-streak method was used to check the antagonistic activity of isolated actinomycetes against bacteria and fungi. Crude extracts from submerged state fermentation were used for the production of antimicrobial compounds. Agar well diffusion method was used for antimicrobial activity of crude extracts against test organisms. The isolates were characterized by morphological, physiological and biochemical methods.

Results: A total of 110 actinomycete isolates were isolated from plastic waste dumping yard. All isolates had shown antimicrobial activity against one or more tested bacteria/fungi. The crude extract of the isolates PD66 (12.2 mm), PD85 (11.5 mm) were most active against methicillin-resistant Staphylococcus aureus, PD4 (14.1 mm), PD66 (15.6 mm) were active against Pseudomonas aeruginosa, whereas the extracts of PD10 (19.2 mm), PD47 (19.8 mm), PD106 (19.1 mm) were active against Candida albicans, PD10 (14.6 mm), PD82 (15.7 mm) active against Saccharomyces cereviciae. The isolates had shown varying morphological, physiological and biochemical characteristics.

Conclusion: The actinomycetes isolated from Kapuluppada plastic waste dumping yard were found to be most promising microorganisms for the production of antibacterial and antifungal antibiotics.



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Berdy J. Bioactive microbial metabolites. J Antibiot 2005;58:1-26.

Ramasamy V, Suban M, Annamalai P. Isolation, characterization and antimicrobial activity of actinobacteria from point climere Coastal region, east coast of India. Int Res J Pharm 2010;1:358-65.

Sundaramoorthi C, Vengadesh PK, Gupta S, Karthick K, Tamilselvi N. Production and characterization of antibiotics from soil-isolated actinomycetes. Int Res J Pharm 2011;2:114-8.

Gonzalez-Franco CA, Robles-Hernandez RY. Actinomycetes as biological control agents of phytopathogenic fungi. Tecnociencia Chihuahua 2009;3:64-73.

Gurung TD, Sherpa C, Agrawal VP, Lekhak B. Isolation and characterization of antibacterial actinomycetes from soil samples of Kalapatthar, Mount Everest Region. Nepal J Sci Technol 2009;10:173-82.

Ogunmwonyi IH, Mazomba N, Mabinya L, Ngwenya E, Green E, Akinpelu DA, et al. Studies on the culturable marine actinomycetes isolated from the Nahoon beach in the Eastern cape province of South Africa. Afr J Microbiol Res 2010;4:2223-30.

Retinowati W. Identification of Streptomyces sp-MWS1 producing antibacterial compounds. Indonesian J Trop Infect Dis 2010;1:82-5.

Khanna M, Solanki R, Lal R. Selective isolation of rare actinomycetes producing novel antimicrobial compounds. Int J Adv Biotechnol Res 2011;2:357-75.

Maleki H, Mashinchian O. Characterization of Streptomyces isolates with UV, FTIR spectroscopy, and HPLC analyses. Bioimpacts 2011;1:47-52.

Jemimah NSV, Srinivasan M, Devi CS. Novel anticancer compounds from marine actinomycetes. J Pharm Res 2011;4:1285-7.

Nonoh JO, Lwande W, Masiga D, Okech MA, Nyende AB, Boga HI. Isolation and characterization of Streptomyces species with antifungal activity from selected national parks in Kenya. Afr J Microbiol Res 2010;4:856-64.

Raja A, Prabakaran P, Gajalakshmi P. Isolation and screening of antibiotic producing psychrophilic actinomycetes and its nature from Rothang hill soil against viridians Streptococcus sp. Res J Microbiol 2010;5:44-9.

Ilic SB, Kontantinovic SS, Todorovic ZB. UV/Vis analysis and antimicrobial activity of Streptomyces isolates. Med Biol 2005;121:44-6.

Adwan G, Mhanna M. Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Middle-East J Sci Res 2008;3:134-9.

Saadoun I, Hameed KM, Moussauui A. Characterization and analysis of the antibiotic activity of some aquatic actinomycetes. Microbios 1999;99:173-9.

Narendra K, Ravi KS, Mishra SK, Singh AK, Pachouri UC. Isolation and screening of soil actinomycetes as a source of antibiotics active against bacteria. Int J Microbiol Res 2010;2:12-6.

Aghamirian RM, Ghiasian AS. Isolation, and characterization of medically important aerobic actinomycetes in the soil of Iran. Open Microbiol J 2009;3:53-7.

Reddy NG, Ramakrishna DPN, Raja Gopal SV. Morphological, physiological and biochemical studies of marine Streptomyces rochei (MTCC 10109) showing antagonistic activity against human pathogenic microorganisms. Asian J Biol Sci 2011;4:1-14.

Waksman SA, Reilly HC, Hariis DA. Streptomyces griseus (Krainsky) Waksman and Henrici. J Bacteriol 1948;56:259.

Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA, Sackin MJ. Numerical classification of Streptomyces and related genera. J Gen Microbiol 1983;129:1743-813.

Hayasaka M, Yoshida Y, Iimura Y. Selective isolation of bioactive soil actinomycetes belonging to the Streptomyces violaceus niger phenotypic cluster. J Appl Microbiol 2004;96:973-81.

You JL, Cao LX, Liu GF, Zhou SN, Tan HM, Lin YC. Isolation and characterization of actinomycetes antagonistic to pathogenic vibrio species from near shore marine sediments. World J Microbial Biotechnol 2005;21:679-82.

Thenmozhi M, Kannabiran K. Studies on isolation, classification and phylogenetic characterization of novel antifungal Streptomyces sp. VITSTK7 in India. Curr Res J Biol Sci 2010;2:306-12.

Sambamurthy R, Ellaiah P. A new streptomycin-producing neomycin (B and C) complex, Streptomyces marinensis (part-1). Hind Antibiot Bull 1974;17:24-8.

Pandey B, Ghimire P, Agrawal VP. Studies on the antimicrobial activity of the actinomycetes isolated from the Khumbu region of Nepal. Appl Microbiol 2008;5:235-61.

Yang KQ, Han L, Vining LC. Regulation of jadomycin-B production in Streptomyces venezuelae Isp5230-involvement of a repressor gene. J Bacteriol 1995;177:6111-7.

Cross T, William ST. Actinomycetes. In: Method in microbiology. 4th ed. London: Academic Press; 1971. p. 29-65.

Tiwari KD. Protocol for actinomycetes studies in RLABB 2008/9. Tridhtuven University Tikiriupr: Central Department of Microbiology, Institution of Science and technology; 2009. p. 21.

Muiru WM, Mutitu EW, Mukunya DM. Identification of selected actinomycetes isolates and characterization of their antibiotic metabolites. J Biol Sci 2008;8:1021-6.

Gebreselema G, Feleke M, Samuel S, Nagappan R. Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pac J Trop Biomed 2013;3:426-35.

Salle AJ. Laboratory Manual on Fundamental Principles of Bacteriology, Mc Graw-Hill, UK; 1948.

Cappuccino JG, Sherman N. Microbiology a Laboratory manua, Addison-Westley Longman, UK; 1996.

Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Systematic Evolutionary Bacteriol 1966;16:313-40.

Abo-Shadi M, Sidkey NM, Al-Mutrafy AM. Antimicrobial agent is producing microbes from some soils’ rhizosphere. J Am Sci 2010;6:915-25.

Abebe B, Feleke M, Berhanu A. Isolation and screening of antibiotic producing actinomycetes from soils in Gondar town, North West Ethiopia. Asian Pac J Trop Dis 2013;3:375-81.

Suthindhiran K, Kannabiran K. Cytotoxic and antimicrobial potential of actinomycete species Saccharopolyspora salina VITSDK4 isolated from the Bay of Bengal Coast of India. Am J Infect Dis 2009;5:90-8.

Usha Y, Koppula S, Vishnuvardhan Z. Bioactive metabolites from marine sediments (Streptomyces species) of three coastal areas. Drug Invent Today 2011;2:114-7.

Sharma SCV, David E. A comparative study on selected marine actinomycetes from pulicat, muttukadu, and ennore estuaries. Asian Pac J Trop Biomed 2012;2:S1827-S1834.

Thakur D, Yadav A, Gogoi BK, Bora TC. Isolation and screening of Streptomyces in the soil of protected forest areas from the states of Assam and Tripura, India, for antimicrobial metabolites. J Med Mycol 2007;17:242-9.

Ilic SB, Konstantinovic SS, Todorovic ZB, Lazic ML, Veljkovic VB, Jokovic N. Characterization and antimicrobial activity of the bioactive metabolites in Streptomycetes isolates. Microbiol 2007;76:421-8.

Smriti Singh, Pramod Kumar, Gopalan N, Bhuvnesh Shrivastava, Kuhad RC, Hotam Singh C. Isolation and partial characterization of actinomycetes with antimicrobial activity against multidrug resistant bacteria. Asian Pac J Trop Biomed 2012;2:S1147-S1150.

Gurung TD, Sherpa C, Agrawal VP, Lekhak B. Isolation and characterization of antibacterial actinomycetes from soil samples of Kalapatthar, Mount Everest Region. Nepal J Sci Technol 2009;10:173-82.

Yucel S, Yemac M. Selection of Streptomyces isolates from turkish karstic caves against antibiotic resistant microorganisms. Pak J Pharm Sci 2010;23:1-6.

Valli S, Suvathi Sugasini S, Aysha OS, Nirmala P, Vinot Kumar P, Reena A. Antimicrobial potential of actinomycetes species isolated from marine environment. Asian Pac J Trop Biomed 2012;9:416-73.



How to Cite

Duddu, M. K., and G. Guntuku. “ISOLATION, SCREENING AND CHARACTERIZATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM KAPULUPPADA PLASTIC WASTE DUMPING YARD, VISAKHAPATNAM”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 11, Nov. 2016, pp. 221-9, doi:10.22159/ijpps.2016v8i11.10110.



Original Article(s)