EVALUATION OF ACUTE AND DERMAL TOXICITY OF ESSENTIAL OIL OF ETLINGERA FENZLII (KURZ) K. SCHUM: AN IN VIVO STUDY

Authors

  • Anju Sudhakaran Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala,India 695562
  • Radha R. K.

Abstract

Objective: The present investigation describes the in vivo acute toxicity and dermal toxicity of essential oil of Etlingera fenzlii (E. fenzlii) in Wistar albino rats.

Methods: The essential oil was extracted by hydro-distillation using Clevenger apparatus. The acute toxicity study was conducted in Wistar albino rats at different doses (175,550 and 2000 mg/kg) while dermal toxicity study was carried out with simple ointment base and essential oil at a dose of 2000 mg/kg body weight. Animals were observed for 14 d and parameters like body weight, feed intake and water intake were studied.

Results: No mortality and no significant changes were observed in body weight and wellness parameters at 175,550 and 2000 mg/kg body weight doses. Further, the dermal absorption with essential oil also did not reveal any treatment-related changes in body weight, food consumption and water intake in any of the animals tested.

Conclusion: These findings suggest that the acute and dermal application of the essential oil of E. fenzlii in rats did not divulge any significant toxicity, and the oil extracts were found to be safe up to 2000 mg/kg in vivo concentration.

Keywords: Etlingera fenzlii, Acute toxicity, Dermal toxicity, Median Lethal Dose (LD 50)

Downloads

Download data is not yet available.

References

Sharief MU, Rao RR. Ethnobotanical studies of shompens-A critically endangered and degenerating ethnic community in great nicobar island. Curr Sci 2007;93:1623-8.

Radha RK, Sam PM, Krishan PN, Seeni S. Phytogeography of the andaman-nicobar islands with special reference to Hornstedtia fenzlii (Kurz) K. Schum. Curr Sci 2010;98:905-7.

Garg SC, Banerjee A. Insect and pest control activity of essential oils. Indian Perfum 1997;41:73-84.

Chang ST, Cheng SS. Antitermitic activity of leaf essential oils and components from Cinnamomum osmopheum. J Agric Food Chem 2002;50:1389-92.

Acute Oral Toxicity [OECD Test Guideline 425] Statistical Programme [AOT425 StatPgm]. Version: 2001. Available from: http://www.oecd.org/oecd/pages/home/displaygeneral/0,3380,ENdocument-524-nodirectorate-no-24-6775-8,FF.html. [Last accessed on 10 Nov 2015]

Acute Dermal Toxicity. OECD guidelines for testing of chemicals; 1987. Available from: http://www.oecd-library.org/ environ-ment/test-no-402-acute-dermaltoxicity 9789264070585 -en; zjessionid=27ckn6k0r 2ekf. x-oecd-live-02. [Last accessed on 10 Nov 2015].

Lawal H, Adewuyi G, Fawehinmi A, Etatuvie S. Chemical evaluation of mosquito repellent formulation prepared from the essential oil of plants. J Nat Prod 2013;6:33-7.

Lansdown ABG. Animal Husbandry. In: Anderson D, Conning DM. editors. Experimental toxicity, the basic issues. 2nd Ed. London, UK: Royal Society of Chemistry; 1993. p. 82–106.

Wang J, Zhou G, Chen C, Yu H, Wang T, Ma Y, et al. Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration. Toxicol Lett 2007;168:176–85.

Celso ARA, Lucas TB, Regina KT, Daisy MF, Luis FB, Mirtes Costa. Cholestrol reduction and lack of genotoxic or toxic effects in mice after repeated 21-day oral intake of lemon grass (Cymbapogn citratus) essential oil. Food Chem Toxicol 2011;2268-72.

Gautam MK, Goel RK. Toxicological study of Ocimum sanctum Linn leaves hematological, biochemical and histopathological studies. J Toxicol 2014;1-10. Doi.org/10.1155/2014/135654. [Article in Press]

Belmain SR, Neal GE, Ray DE, Golob P. Insecticidal and vertebrate toxicity associated with ethnobotanicals used as post-harvest protectants in Ghana. Food Chem Toxicol 2001;39:287-91.

Iversen PO, Nicolaysen G. Water for life. J Norw Med Assoc 2003;123:3402-5.

Deepika Bhatt, Amit Kumar Sachan, Sanjay Jain, Rakesh Barik. Studies on the inhibitory effect of Eucalyptus oil on sebaceous glands for the management of acene. Indian J Nat Prod Resour 2011;2:345-9.

Published

01-07-2016

How to Cite

Sudhakaran, A., and R. R. K. “EVALUATION OF ACUTE AND DERMAL TOXICITY OF ESSENTIAL OIL OF ETLINGERA FENZLII (KURZ) K. SCHUM: AN IN VIVO STUDY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 7, July 2016, pp. 69-72, https://innovareacademics.in/journals/index.php/ijpps/article/view/10328.

Issue

Section

Original Article(s)