118A>G AND IVS2+691G>C POLYMORPHISMS OF OPRM1 GENE HAVE NO INFLUENCE ON COLD-PAIN SENSITIVITY AMONG HEALTHY OPIOID-NAIVE MALAY MALES


Zalina Zahari, Lee Chee Siong, Lee Yeong Yeh, Muslih Abdulkarim Ibrahim, Nurfadhlina Musa, Md Azhar Mohd Yasin, Tan Soo Choon, Nasir Mohamad, Rusli Ismail

Abstract


Objective: Common polymorphisms of the mu-type opioid receptor (OPRM1) including 118A>G and IVS2+691G>C may affect experimental pain responses in healthy subjects, and the effect could be ethnic-dependent. The aim of this study was to investigate the influence of these OPRM1 polymorphisms on cold-pressor pain responses among healthy opioid-naive Malay males.

Methods: Pain-threshold, pain-tolerance, and pain-intensity in response to the cold pressor test (CPT) were measured in healthy opioid-naive Malay males. DNA was extracted from the collected venous blood before PCR-genotyping. Repeated measure analysis of variance (RM-ANOVA) was used to compare CPT responses and OPRM1 polymorphisms (118A>G and IVS2+691G>C) according to their genotypes and allelic additive models, genotype dominant and recessive models, haplotypes, and diplotypes.

Results: A total of 152 participants were recruited. Both 118A>G and IVS2+691G>C polymorphisms were not associated with cold-pressor pain-threshold, pain-tolerance and pain-intensity despite using genotypes and allelic additive models and genotype dominant and recessive models (all p>0.05). Likewise, there were no significant associations between haplotypes and diplotypes for the 118A>G and IVS2+691G>C polymorphisms and the three cold-pain responses (all p>0.05).

Conclusion: The common OPRM1 polymorphisms (i.e., 118A>G and IVS2+691G>C), are not associated with cold-pressor pain responses in healthy opioid-naive Malay males. However, this may be unique for this particular ethnicity. Other polymorphisms may be more relevant for this population, and this should be further investigated.

Keywords: Cold pressor test (CPT), Mu-type opioid receptor (OPRM1), Opioid receptor, mu 1 gene (OPRM1), Pain-threshold, Pain-tolerance, Pain-intensity, Opioid-naive, Male, Malays


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About this article

Title

118A>G AND IVS2+691G>C POLYMORPHISMS OF OPRM1 GENE HAVE NO INFLUENCE ON COLD-PAIN SENSITIVITY AMONG HEALTHY OPIOID-NAIVE MALAY MALES

Date

01-07-2016

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 8, Issue 7, 2016 Page: 73-80

Online ISSN

0975-1491

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90 Views | 78 Downloads

Authors & Affiliations

Zalina Zahari
Department of Pharmacy, Hospital Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia, Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia,
Malaysia

Lee Chee Siong
Department of Emergency Medicine, School of Medical Sciences, Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia

Lee Yeong Yeh
School of Medical Sciences, Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia

Muslih Abdulkarim Ibrahim
Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia5Department of Pharmacology and Toxicology, College of Pharmacy, Hawler Medical University, Hawler, Iraq

Nurfadhlina Musa
Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia,

Md Azhar Mohd Yasin
Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia Department of Psychiatry, School of Medical Sciences, Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia

Tan Soo Choon

Nasir Mohamad
Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia, 7Faculty of Medicine & Health Sciences, Universiti Sultan Zainal Abidin, Kuala Terengganu, Terengganu, Malaysia.

Rusli Ismail
2Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia, Centre of Excellence for Research in AIDS (CERiA), University of Malaya, Kuala Lumpur, Malaysia.


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