IN VITRO ANTIOXIDANT POTENTIAL OF WHOLE PLANT OF <em>ANDROGRAPHIS ECHIOIDES</em> (L.) NEES (ACANTHACEAE)
Objective: Antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of the whole plant of Andrographis echioides have been tested using various antioxidant model systems viz; DPPH (1,1-Diphenyl-2-picryl-hydrazyl), hydroxyl, superoxide, ABTS (2, 2'-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid) and reducing power.
Methods: Total phenolic content was estimated by folin-ciocalteau method. Flavonoids were determined by Aluminium chloride method. In vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts was evaluated by studying DPPH (1,1-Diphenyl-2-picryl-hydrazyl) radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical scavenging activity, ABTS radical cation scavenging activity and reducing power using the standard procedure.
Results: The total phenolic and flavonoids in methanol extract were found to be 1.26g/100 g and 1.18 g/100 g respectively. The methanol extract of Andrographis echioides is found to possess DPPH, hydroxyl, superoxide and ABTS radical cation scavenging activity. The IC50 value of DPPH, hydroxyl, superoxide, and ABTS radical cation scavenging activity were found to be 49.11, 36.18, 37.13 and 38.15 mg/ml respectively. Like the antioxidant activity, reducing the power of the extract increases with increase in concentration.
Conclusion: The finding indicated promising antioxidant activity of crude extracts of the above plant and needs further exploration for their effective are in the both modern and traditional system of medicines.
Keywords: Antioxidant activity, DPPH, ABTS, Phenol, Flavonoid.
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