IN VITRO ANTIOXIDANT POTENTIAL OF WHOLE PLANT OF <em>ANDROGRAPHIS ECHIOIDES</em> (L.) NEES (ACANTHACEAE)

  • A. Agnel Ruba
  • V. R. Mohan Ethnopharmacology Unit, Research Department of Botany, V. O. Chidambaram College, Tuticorin 628008, Tamil Nadu. India

Abstract

Objective: Antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of the whole plant of Andrographis echioides have been tested using various antioxidant model systems viz; DPPH (1,1-Diphenyl-2-picryl-hydrazyl), hydroxyl, superoxide, ABTS (2, 2'-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid) and reducing power.

Methods: Total phenolic content was estimated by folin-ciocalteau method. Flavonoids were determined by Aluminium chloride method. In vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts was evaluated by studying DPPH (1,1-Diphenyl-2-picryl-hydrazyl) radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical scavenging activity, ABTS radical cation scavenging activity and reducing power using the standard procedure.

Results: The total phenolic and flavonoids in methanol extract were found to be 1.26g/100 g and 1.18 g/100 g respectively. The methanol extract of Andrographis echioides is found to possess DPPH, hydroxyl, superoxide and ABTS radical cation scavenging activity. The IC50 value of DPPH, hydroxyl, superoxide, and ABTS radical cation scavenging activity were found to be 49.11, 36.18, 37.13 and 38.15 mg/ml respectively. Like the antioxidant activity, reducing the power of the extract increases with increase in concentration.

Conclusion: The finding indicated promising antioxidant activity of crude extracts of the above plant and needs further exploration for their effective are in the both modern and traditional system of medicines.

Keywords: Antioxidant activity, DPPH, ABTS, Phenol, Flavonoid.

Keywords: Antioxidant activity, DPPH, ABTS, Phenol, Flavonoid.

Downloads

Download data is not yet available.

References

1. Azizova OA. Role of free radical processes in the development of atherosclerosis. Biol Membr 2002;19:451-71.
2. Nagler R, Rezrick A, Shafir Y, Stebadeh N. Free radical related effects and antioxidants in saliva and seram of adolescents with type 1 diabetes melitus. Free Radical Res 2006;40:51-6.
3. Barnham KJ, Masters CL, Bush AI. Nero degenerative diseases and oxidative stress. Nat Rev Drug Discovery 2004;3:205-14.
4. Wickens AP. Aging and the free radical theory. Respir Physiol 2001;128:379-91.
5. Stamler JS, Hausladen A. Oxidative modifications in nitrosative stress. Nat Struct Biol 1998;5:247-9.
6. Nordberg J, Arner ES. Reactive oxygen species, antioxidants and the mammalian thioredoxin system. Free Radical Biol Med 2001;31:1287-312.
7. Li HB, Li D, Zharg Y, Gan RY, Sory FL, Chen F. Antioxidant properties of chinese medicinal plants. In. Reactive oxygen species and antioxidant in higher plants. Ed. Gupta. SD science publishers. Eryuld CT, USA; 2010. p. 331-9.
8. Harman D. Role of free radicals in aging and disease Ann. New York Acad Sci 1992;673:598-620.
9. Rohman A, Riyanto S, Yuniarti N, Saputra WR, Utami R. Antioxidant activity, total phenolic, and total flavonoid of extract and fractions of red fruit (Pardanus conoideus Lam). Int Food Res 2010;17:97-106.
10. Chen C, Pearson MA, Gray IJ. Effects of synthetic antioxidants (BHA, BHT and PG) on the mutagenicity of IQ-like compounds. Food Chem 1992;43:177-83.
11. Nadkarni AK, Nadkarni KM. Indian Materia Medica. Vol. 1. Bombay: Popular Prakashan; 1976. p. 9-10.
12. Qadrie ZL, Beena Jacob, Anandan R, Rajkapoor B, Rahamathulla M. Antibacterial activity of ethanol extract of Indoneesiella echioides evaluated by the filter paper disc method. Pak J Pharm Sci 2009;22:123-5.
13. Krithikar KR, Basu RD. Indian Medicinal Plants. Vol. II. 4th edition. International Book. Distributors, Dehradun; 1975. p. 1325-7.
14. Nazar S, Ravikumar S, Williams GP, Syed Ali M, Suganthi P. Screening of Indian coastal plant extracts for larvicidal activity of Culex quinquefasciatus. Indian J Sci Technol 2009;2:24-7.
15. Pandi kumar P, Ayyanar M, Ignacimuthu S. Medicinal plants used by malasar tribes of coimbatore district, Tamil Nadu. Indian J Traditional Knowledge 2007;6:579-82.
16. Saraf. Phytochemical and antibacterial studies of medicinal plant Costus specious Koer. E J Chem 2010;7:S405-S13.
17. PS Shajeela, V Kalpanadevi, VR Mohan. Potential antidiabetic, hyperlipidaemic and antioxidant effects of Nymphaea pubescens extract in alloxan induced diabetic rats. J Appl Pharm Sci 2012;2:83-8.
18. M Murugan, VR Mohan. Evaluation of phytochemical analysis and antibacterial activity of Bauhinia purpurea L. and Hiptage bengalensis (L.) Kurz. J Appl Pharm Sci 2011;1:157-60.
19. PG Kareru, JM Keriko, AN Gachanja, GM Kenji. Direct detection of triterpenoid saponins in medicinal plants. Afr J Tradit Complementary Altern Med 2008;5:56–60.
20. McDonald S, Prenzler PD, Antolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem 2001;73:73-84.
21. Eom SH, Cheng WJ, Hyoung JP, Kim EH, Chung MI, Kim MJ, et al. Far infrared ray irradiation stimulates antioxidant activity in Vitis flexuosa Thunb. Berries Kor J Med Crop Sci 2007;15:319-23.
22. Shen Q, Zhang B, Xu R, Wang Y, Ding X, Li P. Antioxidant activity in vitro of selenium contained protein from these-enriched. Bifodobacterium animalis 01. Anaerobe 2010;16:380-6.
23. Halliwell B, Gutteridge JMC, Aruoma OI. The deoxyribose method: a simple test to be assayed for determination of rate constants for reaction of hydroxyl radicals. Anal Biochem 1987;65:215-9.
24. Srinivasan R, Chandrasekar MJN, Nanjan MJ, Suresh B. Antioxidant activity of Caesalpinia digyna root. J Ethnopharmacol 2007;113:284-91.
25. Huang MH, Huang SS, Wang BS, Wu CH, Sheu MJ, Hou WC. Antioxidant and anti-inflammatory properties of Cardiopermum halicacabum and its reference compounds ex vivo and in vivo. J Ethnopharmacol 2011;133:743-50.
26. Kumar RS, Hemalatha S. In vitro antioxidant activity of alcoholic leaf extract and subfractions of Alangium lamarckii Thwaites. J Chem Pharm Res 2011;3:259-67.
27. Tresina PS, Mohan VR. Preliminary phytochemical, FT-IR and antibacterial assessment of leaf of Eugenia singampattiana Bedd (Myrtaceae). Int J Adv Res 2014;2:780-7.
28. Sulaiman SF, Yusoff NAM, Eldeem IM, Seow EM, Sajak AAB, Supriatno OKL. Correlation between the total phenolic and mineral contents unit antioxidant activity of eight Malayeian Bananas (Musa sp). J Food Compost Anal 2011;24;1-10.
29. Sri J, Yu J, Pohorly J, Young C, Bragan M, Wu J. Optimization of the extraction of polyphenolics from grapes seed and aqueous ethanol solution. Food Agric Environ 2006;1:42-7.
30. Halliwell B, Gutteridge JMC. Formation of thiobarbituric acid reactive substances from deoxyribose in the presence of iron salts: the role of superoxide and hyroxyl radicals. FEBS Left 1981;128:347-52.
31. Battu GR, Ethadi SR, Veda PG, Swathi PK, Chandika K, Rao VA. Evaluation of antioxidant and anti-inflammatory activity of Euphorbia heyneana Spreng. Asian Pac J Trop Biomed 2011;S191-S-4.
32. Meyer AS, Isaleeen A. Application of enzymes as food antioxidants. Trends Food Sci Technol 1995;6:300-4.
33. Sreejayan N, Rao MNA. The free radical scavenging activity of curcuminoids. Drug Res 1996;46:169.
34. Anitha M, Sakthidevi G, Muthukumaraswamy S, Mohan VR. In vitro antioxidant potential of Cynoglossum zeylanicum (VAHL EX Hornem) Thunb ex Lehm. Whole plant (Boranginaceace). Int J Chem Pharm Sci 2012;3:53-9.
Statistics
216 Views | 1309 Downloads
How to Cite
Ruba, A. A., and V. R. Mohan. “IN VITRO ANTIOXIDANT POTENTIAL OF WHOLE PLANT OF <em>ANDROGRAPHIS ECHIOIDES</Em&gt; (L.) NEES (ACANTHACEAE)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 6, Apr. 2016, pp. 70-75, https://innovareacademics.in/journals/index.php/ijpps/article/view/10544.
Section
Original Article(s)