• Elayne B. F. Pinheiro Departamento de Química Orgânica e Inorgânica
  • Juliana G. A. Silva Departamento de Química Orgânica e Inorgânica
  • Caroline G. Sampaio Instituto Federal de Educação, Ciência e Tecnológia–IFCE, Maracanaú, Ceará, 61.939-140‎
  • Maria I. B. Fernandes Departamento de Química Orgânica e Inorgânica,
  • Maria G. V. Silva Departamento de Química Orgânica e Inorgânica, Departamento de Química Analítica e Físico-Química, Universidade Federal do Ceará, 60455-970, Fortaleza, Ceará, Brasil,


Plectranthus grandis, Barbatusin, Circadian, Seasonal


Objective: This work includes the study of circadian and seasonal variation of barbatusin, active compound present in P. grandis (Plectranthus grandis), through the implementation and validation of an analytical method capable of detecting and quantifying this metabolite.

Methods: High-performance liquid chromatography (HPLC) was performed with a Phenomenex C18 column (250 mm x 4.60 mm-5 µm), a binary gradient of water and acetonitrile (8:2, v/v) at a constant flow rate of 0.8 ml min-1 and ultraviolet-visible spectroscopy (UV-VIS) at 254 nm as detector.

Results: The retention factor for barbatusin was found to be 18.34±0.05 min. The calibration curve was linear (r2>0.999). The intraday and interday precisions of the method were determined, the (Relative Standard Deviation) RSD (%) ranged from 0.74 to 3.53 and from 2.21 to 5.30, respectively. The limits of detection and quantification were 85.30 and 258.40 µg ml-1. The method is simple, precise, accurate and selective and can be routinely used for barbatusin analysis in P. grandis extracts.

Conclusion: The methodology can be applied to the quantification barbatusin of powdered samples P. grandis at any time of year. The method showed excellent results in all steps of the validation demonstrating the possibility of an extensive use of time for the determination with the largest amount of compound derivatization of the same order for future studies.

Keywords: Plectranthus grandis, Barbatusin, HPLC, Quantification, Diterpene


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How to Cite

Pinheiro, E. B. F., J. G. A. Silva, C. G. Sampaio, M. I. B. Fernandes, and M. G. V. Silva. “SEASONAL AND CIRCADIAN VARIATION OF BARBATUSIN IN SAMPLES OF PLECTRANTHUS GRANDIS WILLENSE GROWN IN NORTHEAST BRAZIL”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 7, July 2016, pp. 124-8,



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