Monu Yadav, Milind Parle


Objective: Pain, an unpleasant sensation that we all experience in daily life, is an alert mechanism to prevent impending tissue injury. The animal models employed for screening of analgesic agents include pain-state models based on the use of thermal, mechanical electrical and chemical stimuli. This study was undertaken with an objective to design, develop and fabricate a new animal model for screening analgesics.

Methods: In the present study, a humble attempt is made to develop a new animal model for screening analgesics overcoming the limitations of earlier models. The utility of the newly developed laboratory model (M-model) of pain was compared with already established models.

Results: A simple laboratory model for screening of analgesics was developed in the present study. In this study, endurance time was defined as the time for which, the animals were able to endure the cold surface of ice-floor. The animals assumed a flinching posture and fled to M-Zone when they were unable to withstand the cold surface. Endurance time was significantly and consistently enhanced by different classes of analgesic agents such as pentazocine, butorphanol, tramadol, diclofenac, ketoprofen and meloxicam. The findings obtained using M-model was in line with those obtained using already established models.

Conclusion: An effective animal model for screening analgesics overcoming the limitations of earlier models was developed in this study. This model showed excellent face and predictive validity.

Keywords: Pain, Analgesics, Cold stimuli, M-model, Flinching posture, Endurance time


Pain, Analgesics, Cold stimuli, M-model, Flinching posture, Endurance time

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Pain, Analgesics, Cold stimuli, M-model, Flinching posture, Endurance time



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International Journal of Pharmacy and Pharmaceutical Sciences
Vol 8, Issue 7, 2016 Page: 156-162

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Authors & Affiliations

Monu Yadav

Milind Parle
Pharmacology Division, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125001 (Haryana) India

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