CURCUMIN AMENDS OXIDATIVE STRESS AND ANTIOXIDANTS STATUS IN OLFACTORY LOBES, CEREBRUM, HYPOTHALAMUS-HIPPOCAMPUS, CEREBELLUM AND PONS-MEDULLA OF MICE ACUTELY INTOXICATED WITH LINDANE

Authors

  • Bharti Chaudhary
  • Sonam Agarwal
  • Renu Bist Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India

Keywords:

Keywords, lindane, curcumin, oxidative stress, endogenous antioxidant

Abstract

Objective: Present study ascertains the neuroprotective potential of curcumin in olfactory lobes, cerebrum, hypothalamus-hippocampus, cerebellum and pons-medulla of mice, intoxicated with lindane.

Methods: For the study, mice were divided into four groups. Olive oil was given as a vehicle to the mice of group I. Mice belonging to groups II and III was administered with lindane and curcumin respectively, for 12 h exposure by intraperitoneal injection. In group IV, curcumin was administered 10-15 min prior to exposure of lindane.

Results: Lindane exposure significantly increased the activities of thiobarbituric acid reactive substance (TBARS) (p<0.05) and protein carbonyl content (PCC) (p<0.05) whereas decreased the activity of reduced glutathione (GSH) (p<0.05) and superoxide dismutase (SOD) (p<0.05). Treatment of curcumin alone as well as in combination with lindane significantly declined the level of TBARS and PCC (p<0.05) and increased the activity of GSH and SOD (p<0.05).

Conclusion: Curcumin has neuroprotective potential. It can be used as a therapeutic agent against lindane induced toxicity.

Keywords: Lindane, Curcumin, Oxidative stress, Endogenous antioxidant

Downloads

Download data is not yet available.

References

Pages N, Sauviat MP, Bouvet S, Goudey-Perrière F. Reproductive toxicity of lindane. J Soc Biol 2002;196:325-38.

Aldegunde M, Parafita M, Fernandez Otero MP. Effect of gamma-hexachlorocyclohexane on serotonin metabolism in rat brain. Gen Pharmacol 1983;14:303-5.

Costabeber I, Trindade R, Fries LM. Organochlorine pesticides level in cow milk. Alimentaria 2001;38:127-9.

WHO (World Health Organisation). Environmental Health Criteria 124, Lindane. Published under the joint sponsorship of the United Nations Environment Programme. The International Labour Organisation and the World Health Organisation; 1991.

Dawson VL, Dawson TM. Free radicals and neuronal cell death. Cell Death Differ 1996;3:71-782.

Sharma OP. Antioxidant activity of curcumin and related compounds. Biochem Pharmacol 1976;25:1811-2.

Hatcher H, Planalp R, Cho J, Torti FM, Torti SV. Curcumin: from ancient medicine to current clinical trials. Cell Mol Life Sci 2008;65:1631-52.

Srimal RC, Dhawan BN. Pharmacology of diferuloyl methane (curcumin), a non-steroidal anti-inflammatory agent. J Pharm Pharmacol 1973;25:447-52.

Mukhopadhyay A, Basu N, Ghatak N, Gujral PK. Anti-inflammatory and irritant activities of curcumin analogues in rats. Agents Actions 198;12:508-15.

Negi PS, Jayaprakasha GK, Jagan Mohan Rao L, Sakariah KK. Antibacterial activity of turmeric oil: a byproduct from curcumin manufacture. J Agric Food Chem 1999;47:4297-300.

Al-omar FA, Nagi MN, Abdulgadir MM, Al Joni KS, AL-Majed AA. An immediate and delayed treatment with curcumin prevents forebrain ischemia-induced neuronal damage and oxidative insult in the rat hippocampus. Neurochem Res 2006;31:611-8.

Oguzturk H, Ciftci O, Aydin M, Timurkaan N, Beytur A, Yilmaz F. Ameliorative effects of curcumin against acute cadmium toxicity on male reproductive system in rats. Andrologia 2012;44:243-9.

Siddique YH, Naz F, Jyoti S. Effect of curcumin on lifespan, activity pattern, oxidative stress, and apoptosis in the brains of transgenic drosophila model of Parkinson's disease. BioMed Res Int 2014;1-6. Doi.org/10.1155/2014/606928. [Article in Press]

Kulkarni SK, Dhir A. An overview of curcumin in neurological disorders. Indian J Pharm Sci 2010;72:149-54.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.

Levine R, Garland D, Oliver. Determination of carbonyl content of oxidatively modified proteins. Methods Enzymol 1990;186:464-78.

Ellman GL. Tissue sulfhydryl groups. Arch of Biochem Biophys 1959;82:70-7.

Dhindsa RH, Plumb-Dhindsa P, Thorpe TA. Leaf senescence correlated with increased level of membrane permeability, lipid peroxidation and decreased the level of SOD and CAT. J Exp Bot 1981;32:93–101.

Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 2nd ed. Clarendon Press: Oxford; 1989.

Mediratta PK, Tanwar K, Reeta KH, Mathur R, Benerjee BD, Singh S, et al. Attenuation of the effect of lindane on immune responses and oxidative stress by Ocimum sanctum seed oil (OSSO) in rats. Indian J Physiol Pharmacol 2008;52:171-7.

Ahmed RS, Suke SG, Seth V, Chakraborti A, Tripathi AK, Banerjee BD. Protective effects of dietary ginger (Zingiber Officinalis Rosc.) on lindane-induced oxidative stress in rats. Phytother Res 2008;22:902–6.

Bist R, Bhatt DK. The evaluation of the effect of alpha-lipoic acid and vitamin E on the lipid peroxidation, gamma-amino butyric acid and serotonin level in the brain of mice (Mus musculus) acutely intoxicated with lindane. J Neurol Sci 2009;276:99-102.

Bist R, Bhatt DK. Augmentation of cholinesterases and ATPase activities in the cerebellum and pons-medulla oblongata, by a combination of antioxidants (resveratrol, ascorbic acid, alpha-lipoic acid and vitamin E), in acutely lindane intoxicated mice. J Neurol Sci 2010;296:83-7.

Bist R, Misra S, Bhatt DK. Inhibition of lindane-induced toxicity using alpha-lipoic acid and vitamin E in the brain of Mus musculus. Protoplasma 2010;242:49-53.

Cekik I, Suzek H. Effect of subacute exposure of dichlorvos at sublethal dosages on erythrocyte and tissue antioxidant defense systems and lipid peroxidation in rats. Ecotoxicol Environ Saf 2009;72:905-8.

Kalender SF, Uzun G, Demir F, Kalender Y. Malathion induced hepatotoxicity in rats, the effects of vitamins C and E. Food Chem Toxicol 2010;48:633-8.

Padma VV, Lalitha G, Shirony NP, Baskaran R. Effect of quercetin against lindane induced alterations in the serum and hepatic tissue lipids in wistar rats. Asian Pac J Trop Biomed 2012;2:910-5.

Reed DJ. Status of calcium and thiols in hepatocellular injury by oxidative stress. Semin Liver Dis 1990;10:285-92.

Bano M, Bhatt DK. Ameliorative effect of a combination of vitamin E, vitamin C, alpha-lipoic acid and stilbene resveratrol on lindane induced toxicity in mice olfactory lobe and cerebrum. Indian J Exp Biol 2010;48:150-8.

Singh R, Sharma P. Hepatoprotective effect of curcumin on lindane-induced oxidative stress in male wistar rats. Toxicol Int 2011;18:124-9.

Qualls Z, Brown D, Ramlochansingh C, Hurley LL, Tizabi Y. Protective effects of curcumin against rotenone and salsolinnol-induced toxicity: implications for Parkinson’s disease. Neurotoxic Res 2014;25:81-9.

Prasad SN, Muralidhara. Neuroprotective effect of geraniol and curcumin in an acrylamide model of neurotoxicity in Drosophila melanogaster. Relevance to neuropathy. J Insect Physiol 2014;60:7–16.

Grisham M, McCord J. Chemistry and cytotoxicity of reactive oxygen metabolites. In: Taylor A, Matalon S, Ward P. editors. Physiology of oxygen radicals. Bethesda. Am Physiol Soc; 1986. p. 1-18.

Rao CV, Rivenson A, Simi B, Reddy BS. Chemoprevention of colon cancer by dietary curcumin. Ann N Y Acad Sci 1995;768:201-4.

Published

01-07-2016

How to Cite

Chaudhary, B., S. Agarwal, and R. Bist. “CURCUMIN AMENDS OXIDATIVE STRESS AND ANTIOXIDANTS STATUS IN OLFACTORY LOBES, CEREBRUM, HYPOTHALAMUS-HIPPOCAMPUS, CEREBELLUM AND PONS-MEDULLA OF MICE ACUTELY INTOXICATED WITH LINDANE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 7, July 2016, pp. 244-8, https://innovareacademics.in/journals/index.php/ijpps/article/view/11783.

Issue

Section

Original Article(s)