• Garima Sharma Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, NOIDA, India
  • Shweta Dang Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, NOIDA, India
  • Sanjay Gupta Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, NOIDA, India
  • Reema Gabrani Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, NOIDA, India


Objective: The aim of the current study was to isolate and identify the bacteriocinogenic strain exhibiting broad range antimicrobial activity and antibiofilm activity from soil of animal farms.

Methods: In the current study, bacterial strains were isolated from soil of twelve different regions of animal farm all over India and screened for antimicrobial activity against Staphylococcus epidermidis, Micrococcus luteus, Pseudomonas fluorescence and Escherichia coli. Antibiofilm ability of these selected strains was checked on preformed biofilm of S. epidermidis and in addition biofilm disruption potential was also determined. The potent bacterial strain was identified at molecular level by 16S ribosomal DNA (rDNA) sequencing.

Results: 30 out of 231 strains isolated from soil were selected on the basis of antibacterial activity against S. epidermidis. One potential candidate (GAS 101) exhibited ≥99% inhibition against S. epidermidis, M. luteus, P. fluorescence and E. coli and also showed antibiofilm activity. GAS 101 16S rDNA sequencing data identified it as Bacillus subtilis. The sequence of B. subtilis was submitted to genbank under accession no. KJ564301.

Conclusion: B. subtilis GAS 101 isolated from soil of animal farm showed the antibacterial activity against all indicator organisms and also displayed antibiofilm activity against preformed biofilm and inhibited biofilm formation of S. epidermidis.

Keywords: Sodium orthovanadate, diabetes mellitus, P53, caspase 3


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1. Inweregbu K, Dave J, Pittard A. Nosocomial infections. Contin Educ Anaesth Crit Care Pain 2005;5:14-7.
2. Cotter PD, Ross RP, Hill C. Bacteriocins-a viable alternative to antibiotics? Nat Rev Microbiol 2013;11:95-105.
3. James R, Penfold CN, Moore GR, Kleanthous C. Killing of E. coli cells by E group nuclease colicins. Biochimie 2002;84:381–9.
4. Rajaram G, Manivasagan P, Thilagavathi B, Saravanakumar A. Purification and characterization of a bacteriocin produced by Lactobacillus lactis isolated from marine environment. Adv J Food Sci Technol 2010;2:138-44.
5. Vamanu E, Vamanu A. Viability of the Lactobacillus rhamnosus IL1 strain in simulated gastrointestinal conditions. Int J Pharmacol 2010;l6:732-7.
6. Stein T. Bacillus subtilis antibiotics: structures, syntheses and specific functions. Mol Microbiol 2005;56:845–57.
7. Bizani D, Motta AS, Morrissy JA, Terra R, Souto AA, Brandelli A. Antibacterial activity of cerein 8A, a bacteriocin-like peptide produced by Bacillus cereus. Int Microbiol 2010;8:125-31.
8. Xie J, Zhang R, Shang C, Guo Y. Isolation and characterization of a bacteriocin produced by an isolated Bacillus subtilis LFB112 that exhibits antimicrobial activity against domestic animal pathogens. Afr J Biotechnol 2009;8:5611–9.
9. Teixeira ML, Dalla RA, Brandelli A. Characterization of an antimicrobial peptide produced by Bacillus subtilis subsp. spizezinii showing inhibitory activity towards Haemophilus parasuis. Microbiology 2013;159:980-8.
10. CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. M7-A8. CLSI, Wayne, PA; 2009.
11. Ivanova I, Kabadjova P, Pantev A, Danova S, Dousset X. Detection, purification and partial characterization of a novel bacteriocin Substance produced by Lactoccous lactis subsp. lactis b14 isolated from Boza-Bulgarian traditional cereal beverage. Biocatalysis 2004;6:47-53.
12. Sharma G, Raturi K, Dang S, Gupta S, Gabrani R. Combinatorial antimicrobial effect of curcumin with selected phytochemicals on Staphylococcus epidermidis. J Asian Nat Prod Res 2014;16:535-41.
13. Cullings KW. Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Mol Ecol 1992;1:233-40.
14. Maleki H, Dehnad A, Hanifian S, Khani S. Isolation and molecular identification of Streptomyces spp. with antibacterial activity from northwest of Iran. Biol Impacts 2013;3:129-34.
15. Ziebuhr W, Hennig S, Eckart M, Kränzler H, Batzilla C, Kozitskaya S. Nosocomial infections by Staphylococcus epidermidis: how a commensal bacterium turns into a pathogen. Int J Antimicrob Agents 2006;28:14-20.
16. Otto M. Staphylococcus epidermidis-the accidental pathogen. Nat Rev Microbiol 2009;7:555-67.
17. Eichorst SA, Breznak JA, Schmidt TM. Isolation and characterization of soil bacteria that define Terriglobus gen. nov., in the phylum Acidobacteria. Appl Environ Microbiol 2007;73:2708-17.
18. Mahrous H, Mohamed A, El-Mongy MA, El-Batal AI, Hamza HA. Study bacteriocin production and optimization using new isolates of Lactobacillus spp. isolated from some dairy products under different culture conditions. J Nutr Food Sci 2013;4:342.
19. Tagoe DNA, Baidoo SE, Dadzie I, Tengey D. Potential sources of transmission of hospital acquired infections in the volta regional hospital in Ghana. Ghana Med J 2011;45:22–6.
20. Obritsch MD, Fish DN, MacLaren R, Jung R. Nosocomial infections due to multidrug-resistant Pseudomonas aeruginosa: epidemiology and treatment options. J Pharmacother 2005;25:1353-64.
21. Bonjar S. Evaluation of antibacterial properties of some medicinal plants used in Iran. J Ethnopharmacol 2004;94:301-5.
22. Shin JM, Gwak JW, Kamarajan P, Fenno JC, Rickard AH, Kapila YL. Biomedical applications of nisin. J Appl Microbiol 2016;1201:1449-65.
23. Wu S, Jia S, Sun D, Chen M, Chen X, Zhong J, et al. Purification and characterization of two novel antimicrobial peptides subpeptin JM4-A and subpeptin JM4-B produced by Bacillus subtilis JM4. Curr Microbiol 2005;51:292–6.
24. Bizani D, Motta AS, Morrissy JA, Terra RM, Souto AA, Brandelli A. Antibacterial activity of cerein 8A, a bacteriocin-like peptide produced by Bacillus cereus. Int Microbiol 2005;8:125-31.
25. Alam SI, Kamran M, Sohail M, Ahmad A, Khan AK. Partial characterization of bacteriocin like inhibitory substance from bacillus subtilis BS15, a local soil isolate. Pak J Bot 2011;43:2195-9.
26. Guilhelmelli F, Vilela N, Albuquerque P, Derengowski LD, Silva-Pereira I, Kyaw C. In: Nadia SP, Octavio LF. editors. New edge of antibiotic development: antimicrobial peptides and corresponding resistance. Switzerland: Frontiers Media SA; 2013. p. 63.
27. Abriouel H, Franz CM, Omar NB, Gálvez A. Diversity and applications of Bacillus bacteriocins. FEMS Microbiol Rev 2011;35:201-32.
28. Korenblum E, Sebastián GV, Paiva MM, Coutinho CM, Magalhães FC, Peyton BM, et al. Action of antimicrobial substances produced by different oil reservoir Bacillus strains against biofilm formation. Appl Microbiol Biotechnol 2008;79:97–103.
29. Chopra L, Singh G, Jena KK, Sahoo DK. Sonorensin: a new bacteriocin with potential of an anti-biofilm agent and a food biopreservative. Sci Rep 2015;21:5.
30. Moryl M, Spętana M, Dziubek K, Paraszkiewicz K, Różalska S, Płaza GA, et al. Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria. Acta Biochim Pol 2014;62:725-32.
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How to Cite
Sharma, G., S. Dang, S. Gupta, and R. Gabrani. “IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF BACTERIA HAVING ANTIMICROBIAL AND ANTIBIOFILM ACTIVITY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 10, Aug. 2016, pp. 111-4, doi:10.22159/ijpps.2016v8i10.12338.
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