• Janhavi Ramchandra Rao Department of Pharmaceutical Chemistry, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy Erandwane, Pune, India.
  • Janhavi R Rao Department of Pharmaceutical Chemistry, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy Erandwane, Pune, India.


Objective: The present study was designed with an objective of a simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of Aspirin, Ramipril, Hydrochlorothiazide, Simvastatin and Atenolol from bulk drug and formulation.

Methods: The separation was achieved on a Hypersil Gold column (250 mm X 4.6 mm, 5 µ) as stationary phase with a mobile phase consisting of methanol: water in the ratio of 95:5% v/v at a flow rate of 1 mL/min and UV detection at 230 nm.

Results: The retention times were observed to be 1.983, 2.525, 3.108, 3.867 and 7.833 minutes for Aspirin, Ramipril, Hydrochlorothiazide, Simvastatin and Atenolol, respectively. The method was statistically validated for linearity, recovery, the limit of detection, limit of quantification, accuracy and precision.

Conclusion: Thus, proposed method was found sensitive, precise, accurate and specific and be used for quantitative estimation of Aspirin, Ramipril, hydrochlorothiazide, Simvastatin and Atenolol in commercial pharmaceutical dosage form.


Keywords: Aspirin, Atenolol, Ramipril, Hydrochlorothiazide, Simvastatin, RP-HPLC.


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How to Cite
Rao, J. R., and J. R. Rao. “RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ASPIRIN, RAMIPRIL, HYDROCHLOROTHIAZIDE, SIMVASTATIN AND ATENOLOL FROM PHARMACEUTICAL DOSAGE FORM”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 443-8, https://innovareacademics.in/journals/index.php/ijpps/article/view/1235.
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