CYTOTOXIC BIOACTIVE COMPOUNDS FROM CALOTROPIS GIGANTEA STEM BARK


Kartini Hasballah, Murniana ., Erya ., Ardian .

Abstract


Objective: The present study deals with the cytotoxic activity of n-hexane and ethyl acetate extracts of Calotropis gigantea L. stem bark and its fractions such as A, B, C, D and E fractions on murine leukemia cell line P388.

Methods: The crude extracts of C. gigantea stem bark were prepared using n-hexane and ethyl acetate solvents. The plant extracts were subjected to vacuum liquid chromatography followed by TLC. According to the similarity of stain patterns, the fractions were combined. The extracts and its combined fractions were then subjected for the phytochemical test. Cytotoxic activity of those extracts and its combined fractions were tested using MTT assay. Fraction D was subjected to gravity column chromatography followed by TLC. Then, fractions A, B, and D2 were crystallized and subjected to GC-MS.

Results: The qualitative screening of n-hexane extract of Calotropis gigantea L. stem bark for secondary metabolites showed the presence of terpenoid, flavonoids, phenolics and coumarins. While the ethyl acetate extract contained phenolics, steroids, flavonoids, saponins and coumarins compounds. IC50 values for n-hexane extract and E fraction are 76.29 µg/ml and 18.48 µg/ml, respectively. In the ethyl acetate extract and C fraction obtained IC50 values 57.05 µg/ml and 52.58 µg/ml.

Conclusion: Cytotoxic activity from E fraction of n-hexane extract of C. gigantea stem bark is the most potent and containing flavonoids, phenolics and coumarins. The main components from several compounds of n-hexane extract of C. gigantea are germacrane-A, (-)-globulol, urs-12-ene and veridiflorol. 


Keywords


Calotropis gigantea, Cytotoxic activity, IC50, MTT assay

| PDF | HTML |

References


World Health Organization Cancer Report; 2010.

World Health Organization Cancer Report; 2011.

Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA: A Cancer J Clin 2011;6:69-90.

Jackson BG. Mechanism-based target identification and drug discovery in cancer research. Science 2000;287:1969-73.

Wang Z, Wang M, Mei W, Han Z, Dai H. A New cytotoxic pregnanone from Calotropis gigantea. Molecules 2008;13:3033-9.

Hasballah K, Murniana, Ichsan. Cytotoxic activities from stem bark extracts of Calotropis gigantea L. edited. M Ramli. Aceh International Pharmacy Conference Proceeding, Syiah Kuala University Press: Darussalam, Banda Aceh; 2013. p. 28-35.

Harbone JB. Phytochemical methods-a guide to modern techniques of plant analysis, London: Chapman and Hall; 1998. p. 182-90.

Mantani N, Imanishi N, Kawamata H, Terasawa K, Ochiai H. Inhibitory effect of (+) catechin on the growth of influenza A/PR/8 virus in MDCK cells. Planta Med 2001;67:240-3.

Kawase M, Motohashi N, Satoh K, Sakagami H, Nakashima H, Tani S. et al. Biological activity of persimmon (Diospyros kaki) peel extracts. Phytother Res 2003;17:495-500.

Caragay AB. Cancer-preventive foods and Ingredients. Food Technol 1992;46:65-8.

Hodek P, Trefil P, Stiborova M. Flavonoids-potent and versatile biologically active compounds interacting with cytochrome P450. Chem Biol Interact 2002;139:1-21.

Dwivedi B, Singh A, Mishra S, Singh R, Pant P, Thakur LK, et al. Evaluation of phytochemical constituents by gas chromatography-mass spectroscopy and HPTLC of Calotropis procera. World J Pharm Res 2014;3:708-15.

Mrunal KS, Mahatma OP, Umesh SP, Sanjay KB, Jayesh, Dwivedi. GC-MS Analysis of Calotropis gigantea linn whole plant chloroform extract. J Pharm Biosci 2013;1:26-9.

Subramanian V, Viji VT, Babu MM, Punitha MJ, Citarasu T. Antimicrobial effect of Calotropis procera active principles against aquatic microbial pathogens isolated from shrimp and fishes. Asian Pac J Trop Biomed 2012;2 Suppl 2:812-7.




About this article

Title

CYTOTOXIC BIOACTIVE COMPOUNDS FROM CALOTROPIS GIGANTEA STEM BARK

Keywords

Calotropis gigantea, Cytotoxic activity, IC50, MTT assay

DOI

10.22159/ijpps.2016.v8i9.12430

Date

01-09-2016

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 8, Issue 9, 2016 Page: 111-115

Online ISSN

0975-1491

Statistics

82 Views | 190 Downloads

Authors & Affiliations

Kartini Hasballah
Department of Pharmacology, Faculty of Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia
Indonesia

Murniana .
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh 23111, Indonesia
Indonesia

Erya .
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh 23111, Indonesia
Indonesia

Ardian .
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh 23111, Indonesia
Indonesia


Article Tools



Refbacks

  • There are currently no refbacks.