ANTIHYPERGLYCAEMIC EFFECT OF FICUS DALHOUSIAE MIQ LEAF ETHANOLIC EXTRACT IN ALLOXANâ€“INDUCED DIABETIC RATS
Objective: Ficus dalhousiae Miq. Has been documented for a wide range of uses in Ayurvedic and Unani medicine. The aim of this study was to evaluate the anti-hyperglycaemic effect of FDLEE in alloxan induced diabetic rats.
Methods: Plant material was collected from Tirupati A. P, during the month of March 2013 the leaves were wiped carefully to make them free from dust and foreign material and dried under shade at room temperature. After seven days, the leaves were powdered and passed through a sieve. The powder was weighed (500 gm) and was extracted by maceration process and the solvent was evaporated in a rotavapor at 40Âº- 50Âº C under reduced pressure. The total yield of the extract was 16.5%. Phytochemical screening was carried out for the detection of alkaloids, flavanoids, glycosides, saponins, sterols and tannins by simple qualitative methods. Diabetes mellitus was induced by single i. p injection of freshly prepared solution of Alloxan monohydrate at a dose of 150mg/kg b. w. The animals were kept under observation for 48hr; Blood glucose was measured by glucometer. The rats with blood glucose levels above 250 mg/dl were selected for the experimental studies. FDLEE (100, 200 & 400mg/kg, b. w) was administered orally once a day for a period of 10 days. Body weight and blood glucose levels were determined on different experimental days.
Results: Significant decrease in body weight and increase in blood glucose and lipid profile were observed in diabetic rats. The administration of FDLEE and glibenclamide daily for 10 days reversed body weights and blood glucose significantly.
Conclusion: FDLEE exhibited anti-hyperglycaemic and anti-hyperlipidaemic effects in diabetic rats which supports its use as an adjunct in treatment of diabetes.
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