EFFECT OF PITHECELLOBIUM DULCE BENTH LEAVES IN DEXAMETHASONE INDUCED DIABETIC RATS
Objective: The objective of the present study was to study the effect of Pithecellobium dulce Benth (P. dulce) leaves in dexamethasone-induced diabetic rats.
Methods: The authenticated P. dulce leaves were collected from a local area of Sangli, Maharashtra. The leaves of the plant were extracted with water and ethanol by maceration and soxhelation respectively. Acute toxicity studies of the both extracts were performed using rat and according to OECD 425 guidelines. The dose of 200 mg/kg and 400 mg/kg was selected for further studies. The albino rats were divided into seven groups with five animals in each group. The diabetes was induced by dexamethasone (10 mg/kg, s. c.) and treated with extract and standard drug for 10 d. Then blood glucose, triglyceride, total cholesterol and glycogen level in liver, muscle and kidney were estimated according to standard procedures.
Results: The study revealed that P. dulce at 200 mg/kg and 400 mg/kg showed significant (p Ë‚ 0.05) antidiabetic activity. All the extract treated groups showed a significant reduction in blood glucose level on 11th day when compared to diabetic control group. The significant increase in blood glucose, triglyceride, and total cholesterol level was observed in the diabetic control group when compared to normal control group. The liver and muscle glycogen level was decreased significantly (p Ë‚ 0.05) in the diabetic control group.Conclusion: It can be concluded that P. dulceaqueous and ethanolic extract at two different doses (200 mg/kg and 400 mg/kg) possesses antidiabetic and hypolipidemic activity.
2. Ebrahim AO, Heidar T, Iraj K, Mohammad TG. Dill tablet: a potential antioxidant and antidiabetic medicine. Asian Pac J Trop Biomed 2015;5:720-7.
3. WHO. Definition, diagnosis and classification of diabetes mellitus and its complications, Geneva: World Health Organization Department on Noncommunicable Disease Surveillance: 1999.
4. Alfiani UP, Tanti AS. Antidiabetic activity of durian (duriozibethinus murr.) and rambutan (nephelium lappaceum l.) Fruit peels in alloxan diabetic rats. Procedia Food Sci 2015;3:255â€“61.
5. Satoskar RS, Bhandarkar SD, Rege NN. Pharmacology and pharmacotherapeutics. 21st ed. Mumbai: Popular Prakashan; 2009.
6. Lilik DW, Anak AI, Tri AS. Potential antioxidant and antidiabetic activities of kayukuning (arcangelisia flava). Agric Agric Sci Proc 2016;9:396â€“402.
7. Kirtikar KR, Basu BD. Indian medicinal plants. 2nded. Dehradun: International Book Distributors; 1975.
8. Zapesochnaya GG. Flavonoids of the leaves of Pithecellobium dulce. Khim Prir Soedin 1980;2:252.
9. Megala J, Geetha A. Free radical-scavenging and H+, K+-ATPase inhibition activities of Pithecellobium. Food Chem 2010;121:1120â€“8.
10. Khare CP. Indian medicinal plants an illustrated dictionary. 1sted. New Delhi: Springer (India) Private Limited; 2007.
11. Shanmugakumaran SD, Amerjyothi S, Balakrishna K. Pharmacognostical, antibacterial and antifungal potentials of the leaf extracts of Pithecellobium dulce Benth. Pharmacogn Mag 2006;7:163-7.
12. Murugesansugumaran. Pithecellobium Dulce benth-a review. Pharmainfo net; 2008. p. 6.
13. Mule VS, Potdar VH, Jadhav SD, Disouza JI. Neuropharmacological profile of aqueous and ethanolic extract of pithecellobium dulce benth leaves in mice. Res J Pharmacol Pharmacodyn 2011;3:27-30.
14. Chun W, Feng W, Jiang L, Yuanfeng Z, Xingfu C. A comparison of volatile fractions obtained from loniceramacranthoides via different extraction processes: ultrasound, microwave, soxhlet extraction, hydrodistillation, and cold maceration. Integrative Med Res 2015;4:171â€“7.
15. Carlos GE, Rafael PP, Alfonso AA, Luicita LR, Nancy AH, Eleazar CM. Effects of aqueous and ethanol extract of dried leaves of pseudocalymma alliaceum (bignonaceae) on hematological and biochemical parameters of wistar rats. Asian Pacific J Reproduction 2015;4:129-4.
16. Ismail K, Esra KA, Funda K, Sinan I, Ipek S, Abdullah E, et al. Determination of the regulatory properties of yucca schidigera extracts on the biochemical parameters and plasma hormone levels associated with obesity. Rev Bras Farmacogn 2016;26:246â€“50.
17. Joyce AS, Aline A, Magaiver AS, Claudia AL, Maria CV, Candida AL, et al. Anti-inflammatory effects and acute toxicity of hydroethanolic extract of Jacaranda decurrens roots in adult male rats. J Ethnopharmacol 2012;144:802-5.
18. Jessica LW, Roy EW. Steroid-induced diabetes: a clinical and molecular approach to understanding and treatment. Diabetes Metab Res Rev 2014;30:99-102.
19. Guido DD, Uberto P, Renato P, Valentina V. Glucocorticoids and type 2 diabetes: from physiology to pathology. J Nutr Metab 2012;12:8â€“9.
20. Heather AF, Ronald CK. New mechanisms of glucocorticoid-induced insulin resistance: make no bones about it. J Clin Invest 2012;122:3854-7.
21. James A. The effects of glucocorticoids on insulin action. Hormonal modifiers of insulin action. 1st ed. New York: Diabetes Association Symposium, Part II; 1963.
22. Abdur R. Drug-induced glucose alterations drug-induced hyperglycemia. Diabetes Spectrum 2011;24:23â€“8.
23. Antonio P, Sergio JC, Ignasi S, Rosa MB, Inka M, Ricardo GH. Glucocorticoid-induced hyperglycemia. J Diabetes 2014;6:9â€“20.
24. Jose GG, Leonor GM, Rene RG, David GA, Fernando JL, Hector ET, et al. Hyperglycemia-related to high-dose glucocorticoid use in noncritically ill patients. Diabetol Metab Syndr 2013;5:1â€“7.
25. Adejuvon AA, Olufunmiyano OA. Further evaluation of antihyperglycemic activity of Hunteriaum bellata (K. Schum) Hallier f. seed extract in experimental diabetes. J Ethnopharmacol 2009;2:238â€“3.
26. Nor AY, Mun FY, Hooi KB, Khairul NR, Tri W, Roziahanim M, et al. Antidiabetic and antioxidant activities of Nypafruticans Wurmb. vinegar sample from Malaysia. Asian Pac J Trop Med 2015;8:595â€“605.
27. Jayaprasad B, Sharavanan PS, Sivaraj R. Antidiabetic effect of Chloroxylon swietenia bark extracts on streptozotocin-induced diabetic rats. Benisuef University J Basic Appl Sci 2016;5:61â€“9.
28. Isela EJ, Carlos AT, Dora EA, Luis FR, Carlos El, Jorge LB. Phytochemical screening and hypoglycemic activity of Carica papaya leaf in streptozotocin-induced diabetic rats. Brazilian J Pharmacognosy 2014;24:341-7.