• R. Varadharajan Dept of Pharmaceutical chemistry, Kamalakshi pandurangan College of harmacy,Tiruvannamalai , Tamil Nadu,India.
  • D. Rajalingam Research Centre, Manonmanium Sundaranar University, Tirunelveli , Tamil Nadu, India
  • S. Palani Dept of Biotechnology, Anna Bioresearch Foundation, Arunai Engineering College, Tiruvannamalai, Tamil Nadu, India


Objective: The present investigations were undertaken to identify phytochemicals by GC MS/MS, to evaluate the cardioprotective and antioxidant activity of the ethanolic extract of leaves of Cucumis Callosus against DOX-induced cardiotoxicity in rats.

Methods: GC MS/MS method was carried out for identification of phyto-compounds present in the Cucumis Callosus. The Cardioprotective effect of Cucumis Callosus (CC) was determined by using Doxorubicin (DOX) intoxication of rats as experimental models. The extent of heart damage and effect of the plant extract was assessed by various biochemical parameters like alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total cholesterol (TC) and triglycerides (TGs) in blood serum and concentration of malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-s-transferase (GST) in heart tissue were determined.

Results: In the Cucumis Callosus extract 32 compounds were identified by GC–MS/MS. Doxorubicin is used to bring significant changes in biochemical parameters and antioxidants in the heart. The pretreatment with Cucumis Callosus at two doses (250 mg/kg and 500 mg/kg) to DOX treated rats significantly prevented the altered enzymes SGPT, SGOT, CPK and LDH, lipid profile LDL, VLDL, TGs, HDL, TC and antioxidant SOD, GSH, CAT, GSH-Px and MDA to near normal level. Serum urea, uric acid, and ALP which are increased on DOX administration registered near normal values on pretreatment with Cucumis Callosus.

Conclusion: This study showed that the cardioprotective and antioxidant activity of Cucumis Callosus, therefore scientifically rampart the use of this plant in traditional medicine for treatment of heart diseases.

Keywords: Cardioprotection, Cucumis Callosus, Doxorubicin, Lipoprotein, Antioxidant


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1. Alan R, Gaby MD. Nutritional treatments for acute myocardial infarction. J Alternative Complementary Med 2010;2:113-22.
2. Billingham ME, JW Mason, MR Bristow, JR Daniels. Anthracycline cardiomyopathy monitored by morphologic changes. Cancer Treat Rep 1978:62:865-72.
3. Bristow MR, PD Thompson, RP Martin, JW Mason, ME Billingham, DC Harrison. Early anthracycline cardiotoxicity. Am J Med 1978;65:823-32.
4. Brown MS, JL Goldstein. A receptor-mediated pathway for cholesterol homeostasis. Science 1986;232:34-47.
5. Grundy SM. Cholesterol and heart disease: a new era. JAMA 1986;256:2849-58.
6. Castelli WP, RJ Garrison, PWF Wilson, RD Abott, S Kalousidan, WB Kannel. The incidence of coronary heart disease and lipoprotein cholesterol levels. The framingham study. JAMA 1986;256:2835-8.
7. Myers CE, WP McGuire, RH Liss, I Ifrim, K Grotzinger, RC Young. Adriamycin: the role of lipid peroxidation in cardiac toxicity and tumor response. Science 1977;19:165-7.
8. Zhou R, Q Xu, P Zheng, L Yan, J Zheng, G Dai. Cardioprotective effect of fluvastatin on isoproterenol-induced myocardial infarction in rat. Eur J Pharmacol 2008;586:244-50.
9. El-Beshbishy HA. Hepatoprotective effect of green tea [Camellia sinensis] extract against tamoxifen-induced liver injury in rats. J Steroid Biochem Mol Biol 2005;38:300-6.
10. Seliya AR, Patel NK. Ethnomedicinal uses of climbers from saraswati river region of patan district, North gujarat. Ethnobotanical Leaflets 2009;13:865-72.
11. ERHSS Ediriweera, WD Ratnasooriya. Ayu, newsletters Ayurved University, Sri Lanka; 2009;30:373-91.
12. Goyal M, Sharma SK. Traditional wisdom and value addition prospects of arid foods of the desert region of North West. India J Traditional Knowledge 2009;8:581-85.
13. Kirtikar KR, Basu BD. Indian Medicinal Plants. Vol. II. 4th ed. International Book Distributors, Dehradun; 1975. p. 1325.
14. Singh MP, H Panda. Medicinal Herbs With Their Formulation. S Daya. Publishing Hous; 2005. p. 311-2.
15. Payal Singh Soam, Tribhuwan Singh, Rekha Vijayvergia, C Jayabaskaran. Liquid chromatography-mass spectrometry based profile of bioactive compounds of Cucumis callosus. Eur J Exp Biol 2013;3:316-26.
16. Panda S, Kar A, Sharma P, Sharma A. Cardioprotective potential of N,α-L-rhamnopyranosyl vincosamide, an indole alkaloid, isolated from the leaves of Moringa oleifera in isoproterenol induced cardiotoxic rats: in vivo and in vitro studies. Bioorg Med Chem Lett 2013;23:959-62.
17. Deng FX, Cao JH, Xia ZL, Lin S, Wang XY. The structure of triptodihydroxy acid methyl ester and Wilfortriene. Acta Bot Sin 1987;29:73–6.
18. Zheng YL, Xu Y, Lin JF. Immunosuppressive effects of wilfortrine and Euonine. Yaoxue Xuebao 1989;24:568-72.
19. Duan HQ, Takaishi Y, Imakura Y, Jia YF, Li D, Cosentino LM, et al. Sesquiterpene alkaloids from tripterygium hypoglaucum and tripterygium wilfordii: a new class of potent anti-HIV agents. J Nat Prod 2000;63:357–61.
20. Natelson S, ML Scott, C Beffna. A rapid method for the estimation of urea in biological fluids by means of the reaction between diacetyl and urea. Am J Clin Pathol 1951;21:275-81.
21. Seligson D. ed. Standard methods of clinical chemistry. Vol. 4. New York, NY: Academic Press; 1963. p. 239-47.
22. Mohun A, IJ Cook. Simple methods for measuring serum level of glutamic oxaloacetic and glutamic pyruvic transaminases in routine laboratories. J Clin Pathol 1957;10:394-9.
23. King J. Practical Clinical Enzymology. Dvan. Nostrand Co, London; 1965. p. 22, 83-93.
24. Zlatkis A, B Zak, GJ Boyle. A new method for the direct determination of serum cholesterol. J Clin Med 1953;41:486-92.
25. Foster LV, RT Dunn. Stable reagents for determination of serum triglycerides by a colorimetric Hatzsch condensation method. Clin Chem 1973;19:338-40.
26. Wilson DE, MJ Spiger. A dual precipitation method for quantitative plasma lipoprotein measurement without ultra-centrifugation. J Lab Clin Med 1973;82:473-5.
27. Zhang XZ. Crop physiology research methods. China Agricultural Press: Beijing; 1992. p. 131-207.
28. Rai S, A Wahile, K Mukherjee, BP Saha, PK Mukherjee. Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds. J Ethnopharmacol 2006;104:322-8.
29. Bergmeyer HU, K Gowehn, H Grassel. Enzymes as biochemical reagents. Weinheim: Verlag Chemie; 1974.
30. Hafemann DG, RA Sunde, WG Houestra. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J Nutr 1974;104:580-4.
31. Carlberg I, B Mannervik. Glutathione reductase levels in rat brain. ‎J Biol Chem 1975;250:5475–9.
32. Mohandas J, JJ Marshall, GG Duggin, JS Horvath, D Tiller. Differential distribution of glutathione and glutathione-related enzymes in rabbit kidney: possible interactions in analgesic neuropathy. Cancer Res 1984;44:5086–91.
33. Singal PK, N Iliskovic. Doxorubicin-induced cardiomyopathy. N Engl J Med 1998;339:900-5.
34. Hardina R, Gersl V, Klimtova I, Simunek T, Machacko va J, Adamcova M. Anthracycline-induced cardiotoxicity. Acta Med 2000;43:75.
35. Takacs IE, Matkovics B, Varga SI, Homolay P, Feer G, Seres T. Study of the myocardial antioxidant defense in various species. Pharm Res 1992;25:177.
36. Herman D. The aging process major risk factor for disease and death. Proc Natl Acad Sci 1991;88:5360–3.
37. Mukherjee S, SK Banerjee, M Maulik, AK Dinda, KK Talwar. Protection against acute doxorubicin-induced cardiotoxicity by garlic: role of endogenous antioxidants and inhibition of TNF-α expression. Biomed Central Pharmcol 2003;3:16-9.
38. Li T, Singhal PK. Doxorubicin-induced early changes in myocardial antioxidant enzymes and their modulation by probucol. Circulation 2015;102;2000.
39. Khanna AK, Ramesh C, Kapoor NK, Terminalia arjuna. An ayurvedic cardiotonic regulates lipid metabolism in hyperlipidemic rats. Phytother Res 1996;10:663.
40. Ahmed KK, Rana AC, Dixit VK. Effect of calotropis procera latex on isoproterenol induced myocardial infarction in albino rats. Phytomedicine 2004;11:327.
41. Smith MT, Bellomo G. Relationship between the mitochondrial transmembrane potential, ATP concentration, and cytotoxicity in isolated rat hepatocytes. Arch Biochem Biophys 1990;282:358.
42. Li T, PK Singhal. Doxorubicin-induced early changes in myocardial antioxidant enzymes and their modulation by probucol. Circulation 2000;102:2105-10.
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How to Cite
Varadharajan, R., D. Rajalingam, and S. Palani. “GCMS/MS ANALYSIS AND CARDIOPROTECTIVE POTENTIAL OF CUCUMIS CALLOSUS ON DOXORUBICIN INDUCED CARDIOTOXICITY IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 9, Sept. 2016, pp. 239-45, doi:10.22159/ijpps.2016.v8i9.13292.
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