APPLICATION OF HPTLC FOR THE QUALITATIVE AND QUANTITATIVE ANALYSIS OF AVOBENZONE, OXYBENZONE, OCTINOXATE IN SUNSCREEN CREAM
Keywords:Densitometry, HPTLC, Sunscreen, Qualitative and Quantitative analysis
Objective: This paper presents the development of an improved method for the Qualitative and Quantitative analysis of Avobenzone(AVO), Oxybenzone(OXY) and Octinoxate(OCTI) using high-performance thin-layer chromatography (HPTLC) with densitometric detection.
Methods: Separation was performed on silica gel 60F254plates and RP 60F254plates. The mobile phase is comprised of Actonitrile:Water(18:2) for RP 60F254 plate and Cyclohexane:n-Hexane:Acetone:Diethyl Ether(14:1:2:2) then both plate were scanned at 254nm and 366nm.
Results: The Rf values were 0.55,0.62 for AVO,OCTI respectively separated on silica gel 60F254plates and 0.63 for OXY separated on RP 60F254plate. By using this method recovery of all three sunscreen agents were 92.7%,99.47%,102.4% of OXY,AVO and OCTI respectively.
Conclusion: The developed HPTLC method combined with densitometry was found suitable for Qualitative and Quantitative determination OXY,AVO and OCTI from sunscreen cream without any interference of excipients.
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