PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT POTENTIAL OF EUPHORBIA HETEROPHYLLA L


Keerthana Kesavan, A. Deepa, G. Shobana, G. Jothi, G. Sridharan

Abstract


Objectives: The present study is to evaluate the preliminary phytochemical screening and in vitro antioxidant potential of Euphorbia heterophylla L.

Methods: In vitro free radical scavenging activity of the chosen plant was evaluated by the methods such as DPPH (1, 1-Diphenyl -2-picrylhydrazyl), reducing power assay, Nitric oxide radical scavenging assay, Hydrogen peroxide scavenging assay (H2O2) and superoxide scavenging assay. Preliminary phytochemical screening and quantification of total phenol, ascorbic acid and organic compounds were also studied.

Results: The results showed that the aqueous extract of Euphorbia heterophylla L. has potent antioxidant activities as evidenced by the IC50 values obtained in DPPH (141.11±4.23µg/ml), Hydrogen peroxide (510.03±2.45µg/ml), Superoxide radical (596.94±1.98µg/ml), reducing power (258.08±3.21µg/ml), Nitric oxide (596.94±6.02µg/ml) scavenging assays. Preliminary phytochemical screening of the aqueous extract of the plant shows the presence of various secondary metabolites such as alkaloid, flavonoid, tannin, saponin, sterol, quinone, lignin and coumarin. The carbohydrate (10.29 mg/g), proteins (7.43 mg/g), were present in measurable amount and was considered to be responsible for the nutritional facts and the phenol (3.26mg/g) and ascorbic acid (1.14%) content attribute to its antioxidant activities.

Conclusion: The results of the present study concluded that the aqueous extract of Euphorbia heterophylla L. posses the antioxidant activity.

 


Keywords


Euphorbia heterophylla L, In vitro antioxidant, Phytochemicals.

| PDF | HTML |

References


Halliwell B, Gutteridge J M C. free radical in biology and medicine, third edition, Oxford university press 1998;246:487-98.

Florence TM. The role of free radicals in disease. J Australian and New Zealand J of Ophthalmology 1995;23(1):3-7.

Saini R, Garg V, Dangwal KY. Comparative study of three wild edible fruits of Uttrakhand for antioxidant, antiproliferative activities and polyphenolic composition. Int J of Pharm and Bio Sci 2012;4:158-67.

Shrinivasan R, Chandrasekar MJN, Nanjan MJ, Suresh B. Antioxidant activity of Caesalpinia digyna root. J of Ethnopharmacology 2007;113, 284-91.

Landrault N, Poucheret P, Ravel P, Gasc F, Cros G, Teissedre PL. Antioxidant Capacities and Phenolics Levels of French Wines from Different Varieties and Vintages. J of Agricultural and Food Chemistry 2001;49(7):3341-43.

Subramanion LJ, Zuraini Z, Sasidharan S. Phytochemicals screening, DPPH free radical scavenging and xanthine oxidase inhibitiory activities of Cassia fistula seeds extract. J of Medicinal Plants Res 2011;5(10):1941-7.

Packer LG, Rimbach, Virgili F. Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritima) bark, pycnogenol. J Free Radical Biology and Medicine 1999;27:704-24.

Ali BH, Blunden G, Tanira MO, Nemmar A. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe):a review of recent research. J Food and Chemical Toxicology 2008;46:409-20.

Omale James and Emmanuel T. Phytochemical Composition, Bioactivity and Wound Healing Potential of Euphorbia heterophylla (Euphorbiaceae) leaf extract. Int J on Pharm and Biomedical Res 2010;1(1):54-63.

Falodun A, Okunrobo LO, Uzoamaka N. Phytochemical screening and anti-inflammatory evaluation of methanolic and aqueous extracts of Euphorbia heterophylla Linn (Euphorbiaceae). African J of Biotechnology 2006;5 (6):529-31.

Meenakshi Sundaram M, Karthikeyan K, Sudarsanam D, Brindha P. Antimicrobial and Anticancer Studies on Euphorbia heterophylla. J of Pharmacy Res 2010;3(9):23-32.

Gamble, J.S. Flora of the presidency of Madras, Printed by S.N. Guha Ray at Sree Saraswaty Press Ltd., Achargy Prafulla Chandra Road, Calcutta. 1957.

Brindha P, Sasikala B, Purushothaman KK. Pharmacognostic studies on Merugan kizhangu. J Bulletin of Medio-Ethno Botanical Res 1981;3:84-6.

Shah MT, Begum S, Khan S. Pedo and biogeochemical studies of mafic and ultramfic rocks in the Mingora and Kabal areas, Swat, Pakistan. J Environmental Earth Sci 2009;DOI:10.1007/s12665-009-0253-8.

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. J Lebens Wissen Technology 1995;28:25-30.

Yen GC, Duh PD. Scavenging Effect of Methanolic Extracts of Peanut Hulls on Free Radical and Active Oxygen Species. J of Agricultural and Food Chemistry 1994;42:629-32.

Ruch RT, Cheng SJ, Klaunig JE. Spin trapping of superoxide and hydroxyl radicals. J Methods in Enzymology 1984;105:198-209.

Sreejayan N, Rao MNA. Free radical scavenging activity of curcuminoids. J Drug Res 1996, 46:169.

Govindarajan R, Rastogi S, Vijayakumar M. Studies on antioxidant activities of Desmodium gangeticum. J Biological and Pharm Bulletin 2003;26:1424-7.

Arvind Mungole J, Ravi Awati, Alka Chaturvedi, Prakash Zanwar. Preliminary Phytochemical screening of Ipomoea obscura (L)-A hepatoprotective medicinal plant. Int J of Pharm Tech Res 2010;2(4);2307-12.

Eghdami A, Sadeghi F. Determination of total phenolic and flavonoids contents in methanolic and aqueous extract of Achillea millefolium. The J of Organic Chemistry 2010;2:81-4.

Tanmay kumar koley, charanjit kaur, shweta nagal, shweta walia, Seema jaggi, Sarika. Antioxidant activity and phenolic content in genotypes of Indian jujube (Zizyphus mauritiana Lamk.). Arabian J of Chemistry 2011;1-9.

Einbond LS, Reynertson KA, Luo XD, Basile MJ, Kennelly EJ. Anthocyanin antioxidants from edible fruits. Food Chemistry 2004;84:23-8.

Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of some Scottish plants for free-radical scavenging activity. J Phytotherapy Res 2007;21(7):615-21.

Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK. Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. J Phytochemistry 2003;63:97-104.

Amin I, Tan SH. Antioxidant Activity of Selected Commercial Seaweeds. Malaysian J of Nutrition 2002;8(2):167‐77.

Kazimierz G, Katarzyna S, Barbara B, Beata K, Magda J, Jan O. Antimutagenic activity of anthocyanins isolated from Aronia melanocarpa fruits. J Cancer Letters 1997;119:37‐46.

Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidantive properties of xantha on the autoxidation of soybean oil in cyclodextrin emulsion. J of Agricultural and Food Chemistry 1992;40:945-8.

Mohammad Shahriar, Sadika Akhter, Ismail Hossain Md, Aminul Haque, Mohiuddin Ahmed Bhuiyan. Evaluation of in vitro antioxidant activity of bark extracts of Terminalia arjuna. J of Medicinal Plants Res 2012;6(39), 5286-98.

Kumaran A, Karunakaran RJ. In vitro antioxidant activities of methanolic extract of Phyllanthus species from India. J LWT-Food Sci and Technology 2007;40:322-52.

Gulcin I, Oktay M, Kirecci E, Kufrevioglu OI. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L) seed extracts. J Food Chemistry 2003;83:371-82.

Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT, Hartzfeld PW, Riechel TL. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J of Agricultural and Food Chemistry 1998;46(5):1887-92.

Govindarajan R, Rastogi S, Vijayakumar M. Studies on antioxidant activities of Desmodium gangeticum. J Biological and Pharm Bulletin 2003;26:1424-7.

Curtis JJ, Mortiz M. Serum enzymes derived from liver cell fraction and response to carbon tetrachloride intoxication in rats. J Gastroenterology 1972;62:84-92.

Roback J, Gryglewski RJ. Flavonoids are scavengers of superoxide anion. J Biochemical Pharmacology 1988;37:837-41.




About this article

Title

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT POTENTIAL OF EUPHORBIA HETEROPHYLLA L

Keywords

Euphorbia heterophylla L, In vitro antioxidant, Phytochemicals.

Date

01-09-2014

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 6, Issue 8, 2014 Page: 549-553

Online ISSN

0975-1491

Authors & Affiliations

Keerthana Kesavan
Srimad Andavan Arts and Science, Bharathidasan University.
India

A. Deepa
Research Scholar

G. Shobana
Research Scholar

G. Jothi
Dean-Lifesciences & Head Biochemistry

G. Sridharan
Research Scholar


Article Tools



Refbacks

  • There are currently no refbacks.