CYTOTOXICITY OF CLINACANTHUS NUTANS EXTRACTS ON HUMAN HEPATOMA (HepG2) CELL LINE

Authors

  • Hazrulrizawati Abd Hamid Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang Kuantan, Pahang, Malaysia
  • Izzah Hayati Yahaya Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang Kuantan, Pahang, Malaysia

DOI:

https://doi.org/10.22159/ijpps.2016v8i10.13626

Keywords:

Clinacanthus nutans, Cytotoxicity, Human hepatoma (HepG2) cell line

Abstract

Objective: Clinacanthus nutans is one of the herbs that has been used in Asia as a traditional medicine for the treatment of serious diseases. The aim of this study is to investigate the phytochemical constituents (flavonoids and phenolics) and cytotoxicity against human hepatoma (HepG2) cancer cell lines of C. nutans extracts.

Methods: The fractions from C. nutans were extracted from hexane, methanol, chloroform and ethyl acetate by the solvent-solvent extraction method. The crude extracts (10 mg/ml) were tested against HepG2 cell lines using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Phytochemical screening was done to determine the total phenolic content (TPC) and total flavonoid content (TFC).

Results: Methanol extract showed the strongest cytotoxic activity against HepG2 cell line with IC 50 of 43.9367μg/ml after 24 h of treatment compared to chloroform extract and ethyl acetate, 55.6112µg/ml and 62.0655µg/ml, respectively. Hexane extract formed the lowest cytotoxicity activity with IC50 of 68.3807µg/ml. Total phenolic content (TPC) was found to be highest in chloroform, which was 119.29 mg of gallic acid equivalent (GAE) and total flavonoid content (TFC), methanol was performed the highest value, which is 937.67 mg of butylated hydroxytoluene (BHT).

Conclusion: Different active compounds present in the extracts may contribute different cytotoxicity effects of crude extracts. The relationship data of total phenolic, total flavonoid, and cytotoxic potential of C. nutans, indicates that these plants might contain valuable active compounds as a chemotherapeutic agent. Further investigations to elucidate the chemical structures of active compounds are necessary for potential compounds discovery in drugs.

Downloads

Download data is not yet available.

References

Krishnakumar N, Sulfikkarali N, Rajendra Prasad N, Karthikeyan S. Enhanced anticancer activity of naringenin-loaded nanoparticles in human cervical (HeLa) cancer cells. Biomed Preventive Nutr 2011;1:223-31.

Yong YK, Tan JJ, Teh SS, Mah SH, Ee GCL, Chiong HS, et al. Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines. J Evidence-Based Complementary Altern Med 2013;9. http://dx.doi.org/10.1155/2013/462751

Ochwang’i DO, Kimwele CN, Oduma JA, Gathumbi PK, Mbaria JM, Kiama SG. Medicinal plants used in treatment and management of cancer in Kakamega County, Kenya. J Ethnopharmacol 2014;151:1040-55.

Roosita K, Kusharto CM, Sekiyama M, Fachrurozi Y, Ohtsuka R. Medicinal plants used by the villagers of a Sundanese community in West Java, Indonesia. J Ethnopharmacol 2008;115:72-81.

Aslam MS, Ahmad MS, Mamat AS. A review on phytochemical constituents and pharmacological activities of Clinacanthus nutans. Int J Pharm Pharm Sci 2014;7:30-3.

Dan PNM. Evaluation of biological activities of Clinacanthus nutans (Burm. F.) lindau: International University, Vietnam; 2014.

Sakdarat S, Shuyprom A, Pientong C, Ekalaksananan T, Thongchai S. Bioactive constituents from the leaves of Clinacanthus nutans Lindau. Bioorg Med Chem 2009;17:1857-60.

Lai HY, Ong SL, Rao NK. In vitro lipase inhibitory effect of thirty-two selected plants in Malaysia. Asian J Pharm Clin Res 2014;7:19-24.

Kuppusamy P, Yusoff MM, Parine NR, Govindan N. Evaluation of in vitro antioxidant and antibacterial properties of Commelina nudiflora L. extracts prepared by different polar solvents. Saudi J Biol Sci 2015;22:293-301.

Roslen NA, Alewi NAM, Ahamada H, Rasad MSBA. Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro. Asian Pacific J Trop Biomed 2014;4:545-8.

Peng TW, P’ng Xiu Wen CJ, Han GAA. Effect of methanol extract of Clinacanthus nutans on serum biochemical parameters in rats. J Appl Pharm 2014;1:77-86.

Soares V, Varanda EA, Raddi M. In vitro basal and metabolism-mediated cytotoxicity of flavonoids. Food Chem Toxicol 2006;44:835-8.

Van Der Watt E, Pretorius JC. Purification and identification of active antibacterial components in Carpobrotus edulis L. J Ethnopharmacol 2001;76:87-91.

Published

2016-10-01

How to Cite

Hamid, H. A., and I. H. Yahaya. “CYTOTOXICITY OF CLINACANTHUS NUTANS EXTRACTS ON HUMAN HEPATOMA (HepG2) CELL LINE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 10, Oct. 2016, pp. 293-5, doi:10.22159/ijpps.2016v8i10.13626.

Issue

Section

Short Communication(s)