• Uma Rajeswari Batchu Department of Pharmaceutical Chemistry, Bharat Institute of Technology
  • Kiranmai Mandava Department of Pharmaceutical Chemistry, Bharat Institute of Technology



Xanthine oxidoreductase, Housekeeping enzyme, Purine catabolism, Hyperuricemia, Allopurinol, XOR natural inhibitors


Xanthine oxidoreductase (XOR) is a widely distributed housekeeping enzyme in mammals that catalyzes the last two steps in human purine catabolism to produce uric acid. The enzyme exists as a homodimer with independent electron transfer in each monomer. This has been studied extensively as a major constituent of the milk fat globule membrane (MFGM) which surrounds fat globules in cow's milk even though purine catabolism is the most accepted function of XOR. A huge number of literature highlights on the different catalytic forms of XOR and their importance in the generation of reactive oxygen species/reactive nitrogen species (ROS/RNS) and synthesis of uric acid which are involved in many physiological and pathological processes. However, a slight ambiguity resides in their biochemical functions. The aim of this article was to review the literature published on the structural, catalytical, physiological and pathological role of XOR and to resolve the ambiguity in biochemical processes and to firm up various natural inhibitors of XOR collectively. Uric acid, the product of purine catabolism shows antioxidant activity, and XOR-derived ROS and RNS play a role in innate immunity, milk secretion and also be involved in signaling and metabolism of xenobiotics. Furthermore, XOR is likely to be engaged in pathology because of excessive production of uric acid and ROS/RNS. This review also reports natural XOR inhibitors in plants which inhibit the enzyme to treat XOR associated pathology.


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Linder N, Rapola J, Raivio KO. Cellular expression of xanthine oxidoreductase protein in normal human tissues. Lab Invest 1999;79:967-74.

Rouault, Tracey. Molybdenum-containing iron-sulphur enzymes. Iron-Sulfur Clusters in Chemistry and Biology. USA: Maryland; 2014.

Eger BT, Okamoto K, Enroth C, Sato M, Nishino T, Pai EF, Nishino T. Purification, crystallization and preliminary X-ray diffraction studies of xanthine dehydrogenase and xanthine oxidase isolated from bovine milk. Acta Crystallogr Sect D: Biol Crystallogr 2000;56:1656-8.

Oxidative stress by Drbry and Mckersie university of Guelph. Available from: http://wwwplantstresscom/articles/ oxidative %20stresshtm/1996. [Last accessed on 02 Jun 2016].

Parks DA, Granger DN. Xanthine oxidase: biochemistry, distribution, and physiology. Acta Physiol Scand Suppl 1986;548:87-99.

Claudia Vorbach, Roger Harrison, Mario R Capecchi. Xanthine oxidoreductase is central to the evolution and function of the innate immune system. Trends Immunol 2003;24:9.

Allen RG, Balon AK. Oxidative influence in development and differentiation: an overview of a free radical theory of development. Free Radical Biol Med 1989;6:631-61.

Shalini Kapoor Mehta, Naira Nayeem. Natural xanthine oxidase inhibitors for management of gout: a review. J Med Health Sci 2014;3:4-13.

Bray RC. Molybdenum iron-sulfur flavin hydroxylases and related enzymes. Enzym 1975;12:299-419.

Hille R. The mononuclear molybdenum enzymes. Chem Rev 1996:96:2757–816.

James M Pauff, Jinjin Zhang, Charles E Bell, Russ Hille. Substrate orientation in xanthine oxidase: crystal structure of the enzyme in reaction with 2-hydroxy-6-methylpurine. J Biol Chem 2008;283:4818-24.

Ichida K. Identification of two mutations in human xanthine dehydrogenase genes responsible for classical type I xanthinuria. J Clin Invest 1997;99:2391–7.

Garattini E. Mammalian molybdo-flavoenzymes, an expanding family of proteins: structure, genetics, regulation, function, and pathophysiology. Biochem J 2003;372:15–32.

Nishino T, Okamoto K, Eger BT, Pai EF. Mammalian xanthine oxidoreductase-mechanism of transition from xanthine dehydrogenase to xanthine oxidase. FEBS J 2008;275:3278-89.

Hille R, Nishino T. Flavoprotein structure and mechanism of Xanthine oxidase and Xanthine dehydrogenase. FASEB J 1995;9:995-1003.

Giulia M, Battelli A, Bolognesi, Letizia Polito. Pathophysiology of circulating xanthine oxidoreductase: New emerging roles for a multi-tasking enzyme. Biochim Biophys Acta (BBA) 2014;1842:1502–17.

Godber BL, Schwarz G, Mendel RR, Lowe DJ, Bray RC, Eisenthal R, et al. Molecular characterization of human xanthine oxidoreductase: the enzyme is grossly deficient in molybdenum and substantially deficient in iron-sulphur centers. Biochem J 2005;388:501-8.

Hae Young Chung, Bong Sook Baek, Sang Ho Song, Myoung Sook Kim, Jung Im Huh, Kyung Hee Shim, et al. Xanthine dehydrogenase/xanthine oxidase and oxidative stress. Age Omaha 1997;20:127–40.

Harrison R. Structure and function of xanthine oxidoreductase: where are we now? Free Radical Biol Med 2002;33:774–97.

Godber BL, Doel JJ, Sapkota GP, Blake DR, Stevens CR, Eisenthal R, et al. Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase. J Biol Chem 2000;275:7757-63.

Godber BLJ, Doel JJ, Durgan J, Eisenthal R, Harrison R. A new route to peroxynitrite. A role for xanthine oxidoreductase. FEBS Lett 2000b;475:93–6.

Millar TM, Stevens CR, Benjamin N, Eisenthal R, Harrison R, Blake DR. Xanthine oxidoreductase catalyses the reduction of nitrates and nitrite to nitric acid under hypoxic conditions. FEBS Lett 1998;427:225–8.

Kurosaki M, Li M, Calzi, Scanziani E, Garattini E, Terao M. Tissue and cell-specific expression of mouse xanthine oxidoreductase gene in vivo: regulation by bacterial lipopolysaccharide. Biochem J 1995;306:225–34.

Saksela M, Apatite R, Raivio KO. Xanthine oxidoreductase gene expression and Enzyme activity in developing human tissues. Biol Neonate 1998;74:274–80.

Al-Khalidi UAS, Chaglassian TH. The species distribution of xanthine oxidase. Biochem J 1965;97:318–20.

Wang J, Van Praagh A, Hamilton E, Wang Q, Zou B, Muranjan M, et al. Serum xanthine oxidase: origin, regulation, and contribution to the control of trypanosome parasitemia. Antioxid Redox Signaling 2002;4:161–78.

Riario-Sforza G, Carcassi A, Bayeli PF, Marcolongo R, Marinello E, Montagnani M. Xanthine oxidase activity in the jejunal mucosa of patients with Gout. Boll Soc Ital Biol Sper 1969;45:785–6.

Auscher C, Amory N, Van der Kemp P, Delbarre F. Xanthine oxidase activity in human intestines: histochemical and radiochemical study. Adv Exp Med Biol 1979;122B:197–201.

Della Corte E, Gozzetti G, Novello F, Stirpe F. Properties of the xanthine oxidase from human liver. Biochim Biophys Acta 1969;191:164–6.

Guerciolini R, Szumlanski C, Weinshilboum RM. Human liver xanthine oxidase: nature and extent of individual variation. Clin Pharmacol Ther 1991;50:663–72.

Stirpe F, Ravaioli M, Battelli MG, Musiani S, Grazi GL. Xanthine oxidoreductase activity in human liver disease. Am J Gastroenterol 2002;97:2079–85.

Watts RW, Watts JE, Seegmiller JE. Xanthine oxidase activity in human tissues and its inhibition by allopurinol 4-hydroxypyrazolo [3,4-d] pyrimidine. J Lab Clin Med 1965;66:688–97.

Sarnesto, Linder N, Raivio KO. Organ distribution and molecular forms of human xanthine dehydrogenase/xanthine oxidase protein. Lab Invest 1996;7:48–56.

Bergmann F, Levene F, Tamir I, Rahat M. Oxidation of methyl derivatives of pteridin-4-one, lumazine and related pteridines by bovine milk xanthine oxidase. Biochim Biophys Acta 1977;480:21–38.

Rodkey FL, Ball EG. A rapid test for distinguishing human from cow's milk based upon a difference in their xanthine oxidase content. J Lab Clin Med 1946;3:354–6.

Krenitsky TA, Neil SM, Elion GB, Hitchings GH. A comparison of the specificities of xanthine oxidase and aldehyde oxidase. Arch Biochem Biophys 1972;150:585–99.

Kooij M, Schijns WM, Frederiks CJF, Van Noorden J, James. Distribution of xanthine oxidoreductase activity in human tissues-a histochemical and biochemical study. Virchows Arch B Cell Pathol 1992;63:17–23.

Taibi G, Di Gaudio F, Nicotra CM. Xanthine dehydrogenase processes retinol to retinoic acid in human mammary epithelial cells. J Enzyme Inhib Med Chem 2008;23:317–27.

Pritsos CA. Cellular distribution, metabolism and regulation of the xanthine oxidoreductase enzyme system. Chem Biol Interact 2000;129:195–208.

Beedham C, John Wiley. Molybdenum hydroxylases In enzyme systems that metabolize drugs and other xenobiotics. Ioannides C 2002;147–87.

Meneshian A, Bulkley GB. The physiology of endothelial xanthine oxidase: from urate catabolism to reperfusion injury to inflammatory signal transduction. Microcirculation 2002; 9:161–75.

Ames BN, Cathcart R, Schwiers E, Hochstein P. Uric acid provides an antioxidant defense in humans against oxidant and radical-caused aging and cancer: a hypothesis. Proc Natl Acad Sci USA 1981;78:6858–62.

Berry CE, Hare JM. Xanthine oxidoreductase and cardiovascular disease: molecular mechanisms and pathophysiological implications. J Physiol 2004;555:589–606.

Peden DB. Uric acid is a major antioxidant in human nasal airway secretions. Proc Natl Acad Sci USA 1990;87:7638–42.

Kastenbauer S. Experimental meningitis in the rat: protection by uric acid at human physiological blood concentrations. Eur J Pharmacol 2001;425:149–52.

Becker BF. Uric acid as radical scavenger and antioxidant in the heart. Pflugers Arch 1989;415:127–35.

Becker BF. Towards the physiological function of uric acid. Free Radical Biol Med 1993;14:615–31.

Granger DN, Hollwarth ME, Parks DA. Ischemia-reperfusion injury: the role of oxygen-derived free radicals. Acta Physiol Scand 1986;548:47–63.

Finkel T. Signal transduction by reactive oxygen species in non-phagocytic cells. J Leukoc Biol 1999;65:337-40.

Fuh KC, Meneshian A, Patel CB, Takiar V, Bulkley GB. Signal transduction by reactive oxygen species: alternative paradigms for signaling specificity Surgery. 2002;131:601-12.

Garattini E, Mendel R, Romao MJ, Wright R, Terao M. Mammalian molybdo-flavoenzymes, an expanding family of proteins: structure, genetics, regulation, function and pathophysiology. Biochem J 2003;372:15-32.

Christen S. Marked elevation in cortical urate and xanthine oxidoreductase activity in experimental bacterial meningitis. Brain Res 2001;900:244–51.

Nagler RM. Characterization of the differentiated antioxidant profile of human saliva. Free Radical Biol Med 2002;32:268–77.

Kooij A, Schiller HJ, Schijns N, Van Noorden CJF, Frederiks WM. Conversion of xanthine dehydrogenase to xanthine oxidase in rat liver and plasma at the onset of reperfusion after ischemia. Hepatology 1994;19:1488-95.

Hannah M, Martin, John T, Hancock Vyv, Salisbury, Roger Harrison. Role of Xanthine oxidoreductase as an antimicrobial agent. Infect Immun 2004;72:4933-9.

Bulkley GB. Endothelial xanthine oxidase: a radical transducer of inflammatory signals for reticuloendothelial activation. Br J Surg 1993;80:686.

Bulkley GB. Reactive oxygen metabolites and reperfusion injury: aberrant triggering of reticuloendothelial function. Lancet 1994:344:934-6.

Lipmann F, Owen CR. The antibacterial effect of enzymatic xanthine oxidation. Science 1943;98:246-8.

Keenan TW, S Patton. The structure of milk: implications for sampling and storage. A milk lipid globule membrane handbook of milk composition. In R G Jensen. ed. Academic Press; 1995. p. 5-50.

Adachi T, Fukushima T, Usami Y, K Hirano. Binding of human xanthine oxidase to sulphated glycosaminoglycans on the endothelial cell surface. Biochem J 1993;289:523-7.

Stevens CR, Millar TM, Clinch JG, Kanczler JM, Bodamyali T, Blake. Antibacterial properties of xanthine oxidase in human milk. Lancet 2000;356:829-30.

Rouquette M, Page S, Bryant R, Benboubetra M, Stevens CR, Blake DR, et al. Xanthine oxidoreductase is asymmetrically localized on the outer surface of human endothelial and epithelial cells in culture. FEBS Lett 1998;426:397–401.

Topham RW, Walker MC, Calisch MP. Liver xanthine dehydrogenase and iron mobilization. Biochem Biophys Res Commun 1982;109:1240–6.

Topham RW, Walker MC, Calisch MP, Williams RW. Evidence for the participation intestinal xanthine oxidase in the mucosal processing of iron. Biochem 1982;21:4529–35.

Stirpe F, Higgins T, Tazzari PL, Rozengurt E. Stimulation by xanthine oxidase of 3T3 Swiss fibroblasts and human lymphocytes. Exp Cell Res 1991;192:635–8.

Rao GN, Berk BC. Active oxygen species stimulate vascular smooth muscle cell growth and proto-oncogene expression. Circ Res 1992;70:593–9.

Galli A, Svegliati-Baroni G, Ceni E, Milani S, Ridolfi F, Salzano R, et al. Oxidative stress stimulates proliferation and invasiveness of hepatic stellate cells via a MMP2-mediated mechanism. Hepatology 2005;41:1074–84.

Mcmanaman JL, Palmer CA, Wright RM, Neville MC. Functional regulation of xanthine oxidoreductase expression and localization in the mouse mammary gland: evidence of a role in lipid secretion. J Physiol 2002;545:567–9.

Keenan TW. Milk lipid globules and their surrounding membrane: a brief history and perspectives for future research. J Mammary Gland Biol Neoplasia 2001;6:365-71.

Krenitsky TA, Spector T, Hall W. Xanthine oxidase from human liver: purification and characterization. Arch Biochem Biophys 1986;247:108–19.

Sanders SA, Eisenthal R, Harrison R. NADH oxidase activity of human xanthine oxidoreductase: generation of superoxide anion. Eur J Biochem 1997;245:541–8.

Harrison R. Human xanthine oxidoreductase: in search of a function. Biochem Soc Trans 1997;25:786–91.

Granger DN, Hollwarth ME, Parks DA. Ischemia-reperfusion injury: the role of oxygen-derived free radical. Acta Physiol Scand 1986;548:47–63.

Ledingham SJ, Katayama O, Lachno DR, Yacoub M. Prolonged cardiac preservation Evaluation of the University of Wisconsin preservation solution by comparison with the St Thomas' Hospital cardioplegic solutions in the rat. Circulation 1990;82:IV351–IV358.

Martin HM. Cellular expression of xanthine oxidoreductase. PhD thesis, University of Bath; 2003.

Boueiz A, Damarla M, Hassoun PM. Xanthine oxidoreductase in respiratory and cardiovascular disorders. Am J Physiol Lung Cell Mol Physiol 2008;294:L830-L40.

Weinbroum A, Nielsen VG, Tan S, Gelman S, Matalon S, Skinner KA, et al. Liver ischemia-reperfusion increases pulmonary permeability in the rat: the role of circulating xanthine oxidase. Am J Physiol Gastrointest Liver Physiol 1995;268:988–96.

Houston M, Estevez A, Chumley P, Aslan M, Marklund S, Parks DA, et al. Binding of xanthine oxidase to vascular endothelium kinetic characterization and oxidative impairment of nitric oxide-dependent signaling. J Biol Chem 1999;274:4985–94.

Davies DF, Davies JR, Richards MA. Antibodies to reconstituted dried milk protein in coronary heart disease. J Atheroscl Res 1969;9:103–7.

Lewis WHP, Ng YLE. Human xanthine oxidase antibody levels: variation in males and females in Chinese and Europeans. Med Lab Sci 1991;48:84–8.

Kasper DL, Braunwald E, Fauci AS, Hauser SL, Longo DL, Jameson JL, et al. Princip Intern Med. New York: McGraw-Hill; 2008. p. 156.

Smalley RV, Guaspari A, Haase-Statz S, Anderson SA, Cederberg D, Hohneker JA. Allopurinol: intravenous use for prevention and treatment of hyperuricemia. J Clin Oncol 2000;1:1758–63.

Puig JG, Torres RJ, Mateos FA, Ramos TH, Arcas JM, Buño AS, et al. The spectrum of hypoxanthine-guanine phosphoribosyl transferase HPRT deficiency clinical experience based on 22 patients from 18 Spanish families. Med Balti 2001;80:102–12.

Stout JT, Caskey CT. Hprt: gene structure, expression, and mutation. Annu Rev Genet 1985;19:127–51.

Roger Harrison. Physiological roles of xanthine oxidoreductase. Drug Metabol Rev 2004;36:363–75.

Robert Kmurray. Pyrimidine metabolism. 26 ed. Harpers illustrated Biochemistry; 2012. p. 300.

Hewinson J, Stevens CR, Millar TM. Vascular physiology and pathology of circulating xanthine oxidoreductase: from nucleotide sequence to functional enzyme. Redox Rep 2005;9:71-9.

Pacher P, Nivorozhkin A, Szabó C. Therapeutic effects of xanthine oxidase inhibitors: renaissance half a century after the discovery of allopurinol. Pharmacol Rev 2006;58:87–114.

Cejkova J, Vejrazka M, Platenık J, Stıpekb S. Age-related changes in superoxide dismutase, glutathione peroxidase, catalase, and xanthine oxidoreductase/xanthine oxidase activities in the rabbit cornea. Exp Gerontol 2004;39:1537–43.

Lin HC, Tsai SH, Chen CS, Chang YC, Lee CM, Lai ZY, et al. Structure–activity relationship of coumarin derivatives on xanthine oxidase inhibiting and free radical-scavenging activities. Biochem Pharmacol 2008;75:1416–25.

Okamoto K, Eger BT, Nishino To, Kondo S, Pai EF, Nishino TA. An extremely potent inhibitor of xanthine oxidoreductase crystal structure of the enzyme-inhibitor complex and mechanism of inhibition. J Biol Chem 2003; 278:1848–55.

Bruce SP. Febuxostat: a selective xanthine oxidase inhibitor for the treatment of hyperuricemia and gout. Ann Pharmacother 2006;40:2187-94.

Becker MA, Schumacher Jr HR, Wortmann RL, Macdonald PA, Eustace D, Palo WA, et al. Febuxostat compared with allopurinol in patients with hyperuricemia and gout. N Engl J Med 2005;353:2450–61.

Schumacher HR Jr. Febuxostat: a non-purine, selective inhibitor of xanthine oxidase for the management of hyperuricemia in patients with gout. Expert Opin Investig Drug 2005;14:893-903.

Kurtis Frank Sol. West, Milk thistle and hepatoprotection: A review of efficacy and safety. Available from: https:// examine come/supplements/milk-thistle/2011. [Last accessed on 04 Jun 2016].

Shivraj h nile, Cn khobragrde. Phytochemical analysis, antioxidant and xanthine oxidase inhibitory activity of Tephrosia purpurea linn. root extract. Indian J Nat Prod Resour 2010;2:52-8.

Kandhasamy Sowndhararajan, Jince Mary Joseph, Dharmar Rajendrakumaran. In vitro xanthine oxidase inhibitory activity of methanol extracts of Erythrina indica lam Leaves and stem bark. Asia Pacific J Trop Biomed 2012;s1415-s1417.

Uma maheswari M, Asokkumar K, Sivashanmugam AT, Remyaraju A, Subhadradevi V, Ravi TK. In vitro xanthine oxidase inhibitory activity of the fractions of Erythrina stricta roxb. J Ethnopharmacol 2009;124:646-8.

Tristan P Dew, Andrea J Day, Michael RA Morgan. Xanthine oxidase activity in vitro: effects of food extracts and components. J Agric Food Chem 2005;53:6510-5.

Http://wwwgoutpalcom/2728/gout-cures-natural-xanthine-oxidase-inhibitors. [Last accessed on 04 Jun 2016].

Yasser Bustanji, Mohammad Hudaib, Khaled Tawaha, Mohammad K Mohammad, Ihab Almasri, Saja Hamed, Sawsan Oran. In vitro xanthine oxidase inhibition by selected jordanian medicinal plants. Jordan J Pharm Sci 2011;4:1.

Govindappa M, Sadananda TS, Channabasava R, Vinay B Raghavendra. In vitro anti-inflammatory, lipoxygenase, xanthine oxidase and acetylcholinesterase inhibitory activity of Tecoma stans l Juss Ex kunth. Int J Pharm Biol Sci 2011;2:275-85.

Subramanian L Jothy, Zakaria Zuraini, Sreenivasan, Sasidharan. Phytochemicals are screening, DPPH free radical scavenging and xanthine oxidase inhibitory activities of Cassia fistula seeds extract. J Med Plant Res 2011;5:1941-19.

Kong LD, Abliz Z, Zhou CX, Li LJ, Cheng CH, Tan RX. Glycosides and xanthine oxidase inhibitors from Conyza bonariensis. Phytochem 2001;58:645-51.

Chin-Hui Chen, Hsiu-Chen Chan, Yi-Tsu Chu, Hsin-Yi Ho, Pi-Yu Chen, Tzong-Huei Lee, et al. Antioxidant activity of some plant extracts towards xanthine oxidase, lipoxygenase, and Tyrosinase. Molecules 2009;14:2947-58.

Marie Bräunlich, Rune Slimestad, Helle Wangensteen, Cato Brede, Karl E Malterud. Extracts, anthocyanins and procyanidins from Aronia melanocarpa as radical scavengers and enzyme inhibitors. Nutrients 2013;5:663–78.

Li DQ, Zhao J, Li SP, Zhang QW. Discovery of xanthine oxidase inhibitors from a complex mixture using an online, restricted-access material coupled with column-switching liquid chromatography with a diode-array detection system. Anal Bioanal Chem 2014;406:1975-84.

Li D, Li S, Zhao J. Screening of xanthine oxidase inhibitors in complex mixtures using online HPLC coupled with post column fluorescence-based biochemical detection. J Sep Sci 2014;37:338-44.

Kong LD, Cai Y, Huang WW, Christopher HK, Cheng RX. Tan. Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout. J Ethnopharmacol 2000:73:199–207.

Boumerfeg S, Baghiani A, Djarmouni M. Inhibitory activity on xanthine oxidase and antioxidant properties of Teucrium polium L extracts. Chin Med 2012;3:30–41.

Tomonori U, Akio S, Takami K. Xanthine oxidase inhibitors from the leaves of Lagerstroemia speciosa L. Pers. J Ethnopharmacol 2004;93:391–5.

Chiang HC, Lo YJ, Lu FJ. Xanthine oxidase inhibitors from the leaves of Alsophila spinulosa hook try on. J Enz Inhib 1994; 8:61–71.

Chen L, Wang W, Wang Y. Inhibitory effects of lithospermic acid on proliferation and migration of rat vascular smooth muscle cells. Acta Pharmacol Sin 2009;30:1245–52.

Jiao RH, Ge HM, Shi DH, Tan RX. An apigenin-derived xanthine oxidase inhibitor from palhinhaeacernua. J Nat Prod 2006;69:1089–91.

Wang SY, Yang CW, Liao JW, Zhen WW, Chu FH, Chang ST. Essential oil from leaves of Cinnamomum osmophloeum acts as a xanthine oxidase inhibitor and reduces the serum uric acid levels in oxonate-induced mice. Phytomedicine 2008;15:940–5.

Intisar Reehem Alsultanee, Mufeed Jalil Ewadh, Maha Fahdil Mohammed. Novel natural anti-gout medication extracts from Momdica charantia. J Nat Sci Res 2014;4:17.

Spanou C, Veskoukis AS, Kerasioti T, Kontou M, Angelis A. Flavonoid glycosides isolated from unique legume plant extracts as novel inhibitors of xanthine oxidase. Plos One 2012;7:3.

Nguyen MT, Awale S, Tezuka Y, Ueda J, Tran QL, Kadota S. Xanthine oxidase inhibitors from the flowers of Chrysanthemum sinense. Planta Med 2006;72:46-51.

Sevilay C, Levent C, Kadir TA. Inhibition of xanthine oxidase by Caulerpenynefrom caulerpaprolifera. Turk J Biochem 2012;37:445–51.

Yang CW, Tseng YH, Tsay HS, Kuo YH, Yang S. Lonicerahypoglauca inhibits xanthine oxidase and reduces serum uric acid in mice. Planta Med 2009:75:302-6.

Boumerfeg S, Baghiani AM, Amen D, Adjadj M, Belkhiri F. Inhibitory activity on xanthine oxidase and antioxidant properties of Teucrium polium. Chin Med 2012;3:30-41.

Nile SH, Khobragade CN. Phytochemical analysis, antioxidant and xanthine oxidase inhibitory activity of Tephrosia purpurea Linn root extract. Indian J Nat Prod Resour 2011;2:52-8.

Hudaib MM, Tawaha KA, Mohammad MK, Assaf AM, Issa AY, Alali FQ, et al. Xanthine oxidase inhibitory activity of the methanolic extracts of selected Jordanian medicinal plants. Pharmacog Mag 2010;7:320-4.

Havlik J, Raquel Huebra GDL, Hejtmankova K, Fernandez J, Simonova J, Melich M, et al. Xanthine oxidase inhibitory properties of czech medicinal plants. J Ethnopharmacol 2010;132:461-5.

Ványolós A, Gyapai OO, Támadi T, Hohmann J. Screening of hungarian mushrooms for xanthine oxidase inhibitory activity. Planta Med 2013;13:9.

Guerrero RO, Guzman AL. Inhibition of xanthine oxidase by puerto rican plant extracts. P R Health Sci J 1998;17:359-6.

Flemmig J, Kuchta K, Arnhold J, Rauwald HW. Olive leaf extract has xanthine oxidase inhibiting activity which confirms its traditional use for gout. Phytomedicine 2011;18:561-6.

Kong LD, Cai Y, Huang WW, Cheng CH, Tan RX. Cinnamon cassia type Polygonum cuspidatum source of resvertrol demonstrated significant inhibition of xanthine oxidase indicating its potential for the treatment of gout. J Ethnopharmacol 2000;73:199-207.

Ling X, Bochu W. A review of phytotherapy of gout: the perspective of new pharmacological treatments. Pharmazie 2014;69:243–56.

Ajay Kumar, Wamik Azmi. Phytomedicine: a novel alternative for treatment of gout. Ann Phys Med 2014;3:80-8.

Rini Hendriani, Elian Yulinah Sukandar, Kusnandar Anggadiredja, Sukrasno. Invitro evaluation of xanthine oxidase inhibitory activity of selected medicinal plants. Int J Pharm Clin Res 2016;8:235-8.

Wahyuningsih, Elin Yulinah Sukandar, Sukrasno. In vitro xanthine oxidase inhibitor activity of ethanol extract and fraction roselle calyx (Hibiscus sabdariffa L.). Int J Pharm Clin Res 2016;8:619-22.



How to Cite

Batchu, U. R., and K. Mandava. “BIOCHEMICAL ROLE OF XANTHINE OXIDOREDUCTASE AND ITS NATURAL INHIBITORS: AN OVERVIEW”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 10, Oct. 2016, pp. 57-65, doi:10.22159//ijpps.v8i10.13927.



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