• V. J. Vishnuvathan SRM college of pharmacy, Kattankulathur, Kancheepuram District, Tamilnadu India
  • K. S. Lakshmi SRM college of pharmacy, Kattankulathur, Kancheepuram District, Tamilnadu India
  • A. R. Srividya SRM college of pharmacy, Kattankulathur, Kancheepuram District, Tamilnadu India



Formononetin, Antioxidant activity, DPPH, Hydrogen peroxide radical scavenging activity, Hydroxyl radical scavenging activity, Nitric oxide radical scavenging activity, Superoxide radical scavenging activity


Objective: Now the important field of research in phytomedicine is to search a new plant source as well as new phytoconstituents that have fewer side effects and low cost with the free radical scavenging activity.

Methods: Different in vitro models such as DPPH, hydroxyl radical, hydrogen peroxide radical, nitric oxide radical and superoxide radical scavenging activity were performed using different concentrations of formononetin ranging from 0.1-50 μg/ml.

Results: Formononetin showed only 84.39% inhibitory activity against DPPH radical and it was found to be 16 % less than the butylated hydroxytoluene with the IC 50 value of 4.65 μg/ml concentration. In scavenging hydroxyl radicals, formononetin inhibited only 67.63% with the IC50 value of 9.48 μg/ml concentration and it showed 12 % lesser inhibitory activity than standard alpha-tocopherol. Formononetin could able to scavenge maximum 73.53% of hydrogen peroxide radicals with the IC50 value of 4.75 μg/ml and its activity of scavenging hydrogen peroxide were found to be 10 % less than the standard ascorbic acid. Formononetin showed 94.33 % nitric oxide radical inhibitory activity with the IC50 value of 5.0 μg/ml and it showed 6% lesser activity when compared to standard quercetin. Formononetin showed 94.79 % activity against the scavenging of superoxide radical with the IC50 value of 4.27 μg/ml. Superoxide inhibitory activity of formononetin was 5.3 %less than the standard quercetin.

Conclusion: All these results suggested that, formononetin is a good natural antioxidant which is capable of scavenging almost all types of free radicals. So this could be used to treat various diseases like diabetes, atherosclerosis, cancers, the aging and cardiovascular disease which is being caused by free radicals.


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Wulf Droge. Free radicals in the physiological control of cell function. Physiol Rev 2002;82:47-95.

Devasagayam TPA, Tilak JC, KK Boloor, Ketaki S Sane, Saroj S Ghaskadbi, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Phys India 2004;52:794-804.

Srividya AR, Dhanabal SP, Sathish Kumar MN, Vishnuvarthan VJ. The relationship between the Curcumin and antioxidant activity in Curcuma aromatica and Curcuma zedoaria rhizomes. J Free Radical Antioxidants Photon 2013;139:186-98.

Ammayappan rajam Srividya, Sangai Palanisamy Dhanabal, Vaithiyalingam Jagannathan Vishnuvarthan. Mutagenicity/ Antimutagenicity of plant extracts used in traditional medicine: a review. World J Pharma Res 2012;2:236-59.

Dharani B, Sumathi S, Siva Prabhu J, Padma PR. In vitro antioxidant potential of Prosopis cineraria leaves. J Nat Prod Plant Resour 2011;1:26-3.

Srividya AR, Dhanabal SP, Mistra VK, Suja G. Antioxidant and antimicrobial activity of Alpinia officinarum. Indian J Pharm Sci 2010;72:145-8.

Tushar Kanti Bera, Kausik Chatterjee Debidas Ghosh. In vitro antioxidant properties of hydroalcoholic extract of the seeds of Swietenia mahagoni. Biomarkers Genomic Med 2014;1:1-7.

Ammayappan Rajam Srividya, Abel Shalom, Raghu Chandrasekhar, Pottakadu Vijayan. Antioxidant, antimicrobial and in vitro cytotoxicity studies of Tithonia Diversifolia A. Grey. Int J Pharm Sci 2009;1:276-9.

Srividya AR, Shalom A, Chandrasekhar R, Vijayan P. Cytotoxic, antioxidant and antimicrobial activity of Polygonum Chinensis Linn. Int J Pharm Sci Nanotechnol 2012;4:1569-76.

Srividya AR, Aishwarya SN, Vishnuvarthan VJ. Evaluation of Antioxidant, Antimicrobial and cytotoxicity activity of Acorus Calamus Linn. Int J Pharmacol Res 2014;3:114-25.

Booth NL, Yao P, Totura S, Deng YF, Hedayat AS, Bolton JL, et al. Seasonal variation of red clover (Trifolium pratense L., Fabaceae) Overk CR. Isoflavones and estrogenic activity. J Agric Food Chem 2006;54:1277–82.

Databases on Food Phytochemicals and their health promotions. Available from: compound/formononetin#section=Names-and-Identifiers. [Last accessed on 20 Aug 2015]

Chemical comound database. Available from: www.Molbase. com/en/name-%20Formononetin %20Glucuronide.html. [Last accessed on 20 Jul 2015].

Lipinski B. Pathophysiology of oxidative stress in diabetes mellitus. J Diabetes Complications 2001;15:203-10.

Glugliano D, Cerriello A, Paolisso G. Diabetes mellitus, hypertension and cardiovascular disease: which role for oxidative stress. Metabolism 1995;44:363-8.

Vasan S, Foiles P, Founds H. Therapeutic potential of breakers of advanced glycation end products-protein crosslinks. Arch Biochem Biophys 2003;419:89-96.

Lele RD. The human genome project: its implications in clinical medicine. J Assoc Physicians India 2003;51:373-80.

Lele RD. Hypertension: molecular approach. J Assoc Physicians India 2004;52:53-62.

Chopra MU, McLoone M, O’Neill N, Williams DI. Fruit and vegetable supplementation-effect on ex-vivo LDL oxidation in humans, In natural antioxidant and food quality in atherosclerosis and Cancer Prevention. 1st ed. The Royal Society of Chemistry. Cambridge: UK; 1996.

Croce CM. How can we prevent cancer? Proc Natl Acad Sci USA 2001;98:10986-8.

Surh YJ. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer 2003;3:768-80.



How to Cite

Vishnuvathan, V. J., K. S. Lakshmi, and A. R. Srividya. “STUDY OF ANTIOXIDANT ACTIVITY OF FORMONONETIN BY IN VITRO METHOD”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 2, Feb. 2017, pp. 273-8, doi:10.22159/ijpps.2017v9i2.13964.



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