VARIATIONS IN PHYTOCONSTITUENTS AND ANTIMICROBIAL ACTIVITIES IN ECOTYPES OF OXALIS CORNICULATA L. AND OXALIS DEBILIS KUNTH

Authors

  • Eleena Panda P.G. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar 751004, Odisha, India
  • Chinmay Pradhan P.G. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar 751004, Odisha, India
  • Anath Bandhu Das P.G. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar 751004, Odisha, India

DOI:

https://doi.org/10.22159/ijpps.2016v8i10.14069

Keywords:

Antimicrobial activity, Genetic diversity, HPTLC, Oxalis, Phytochemical screening

Abstract

Objective: The present study aims at the comparative analysis of phytoconstituents and antimicrobial activities of four ecotypes of Odisha, India, each of Oxalis corniculata L. (OC-Eco-1, OC-Eco-2, OC-Eco-3, OC-Eco-4) and Oxalis debilis Kunth. (OD-Eco-1, OD-Eco-2, OD-Eco-3, OD-Eco-4) of the family Oxalidaceae.

Methods: The ecotypes were collected from four districts (Balasore, Bhadrak, Jajpur, Khurda) of Odisha, India. The qualitative phytochemical screening was done to test glycosides, saponins, tannins and terpenoids. Further analysis of phytochemicals was also screened through TLC and HPTLC. Antimicrobial potentiality of leaf extract of various ecotypes was studied against Bacillus subtilis, Pseudomonas aeruginosa and Streptococcus epidermis by disc diffusion method.

Results: Tannin and terpenoids estimation revealed that significantly high amount of phytoconstituents were present in OD-Eco-3 of O. debilis. The methanolic leaf extracts of each ecotype were screened for qualitative analysis of phytochemical through TLC and HPTLC that showed maximum 16 compounds in O. debilis (OD-Eco-3) a new report, as compared to O. corniculata. All the four ecotypes of O. debilis showed some new HPTLC bands (Rf 0.96, 0.90, 0.90, 0.81) as compared to O. corniculata that occupied 7.70%, 22.38%, 23.79% and 34.42% of peak area respectively. Cluster analysis on the basis of HPTLC banding pattern showed a close affinity among ecotypes of each species. Crude extracts showed antibacterial activity against B. subtilis, P. aeruginosa and S. epidermis. Leaf extracts of ecotypes of O. corniculata showed zones of inhibition and MIC better than that of O. debilis against S. epidermis.

Conclusion: The findings significantly reported some new compounds for the first time in O. debilis. The study also indicated a promising potential of antimicrobial activity of O. debilis which was the first report.

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References

Pradhan C, Mohanty M, Rout A, Das AB, Satapathy KB, Patra HK. Phytoconstituent screening and comparative assessment of antimicrobial potentiality of Artocarpus altilis fruit extracts. Int J Pharm Pharm Sci 2013;5:840-3.

Cragg GM, Newman DJ, Sander KM. Natural products in drug discovery and development. J Nat Prod 1997;60:52-60.

Goyal BR, Goyal RK, Mehta AA. Phytopharmacognosy of Archyr-anthes aspera: a review. Pharmacogn Rev 2008;1:143-50.

Pradhan C, Mohanty M, Rout A. Phytochemical screening and comparative bio-efficacy assessment of Artocarpus altilis leaf extracts for antimicrobial activity. Front Life Sci 2013;6:71-6.

Kirtikar, Basu. Indian medicinal plants. MS periodical experts. 3rd Ed. New Delhi; 1975. p. 1-437.

Kumar A, Niketa, Rani S, Sagwal S. An absolute review on Oxalis corniculata Linn. Int J Res Pharm Biomed Sci 2012;3:1173-88.

Kumar A, Chaudhary P, Verma KN, Kumar P, Lalit. TLC based phytochemical and antioxidant analysis of Oxalis corniculata L. Int Res J Pharm Appl Sci 2013;3:6-12.

Ramappa R, Mahadevan GD. In vitro antimicrobial activity of various plant latex against resistant human pathogens. Int J Pharm Pharm Sci 2011;3:70-2.

Sarma A, Sarmah P, Dolai DK, Medhi P. Oxalis debilis var. corymbosa (DC.) Lourteig and Oxalis corniculata L. a comparative study of nutraceutical properties. Int J Curr Res 2015;7:11307-10.

Denton MF. A monograph of Oxalis, section Ionoxalis (Oxalidaceae) in North America. Publication of the Michigan State University Museum, Biological Series 1973;4:455–615.

Lourteig A. Oxalis L. subgenera Monoxalis (Small) Lourt. Oxalis trifidus Lourt. Bradea 2000;7:201–629.

Haines HH. The botany of Bihar and Orissa. Adlard and Son and West Newman Ltd; 1988. p. 156-1237.

Evans WC. An index of medicinal plants. A Textbook of Pharmacognosy. 14th Ed; 1997;7:12-4.

Kokate CK. Plant constituents in Practical Pharmacognosy; 2001. p. 107-10.

Mace Gorbach SL. Anaerobic bacteriology for clinical laboratories. Pharmacognosy 1963;23:89-91.

Rohlf FJ. Ntsys-PC. Numerical taxonomy and multivariate analysis system Version 1. 80-Setauket, NY, Exeter Software; 1993.

Bauer AW, Perry DM, Kirby WMM. Single disc antibiotic sensitivity testing of Staphylococci. AMA Arch Intern Med 1959;104:208–16.

Prescott LM, Harley JP, Klein DA. Microbiology. 6th ed. New York: McGraw-Hill Publishers; 2005. p. 782–86.

Raghavendra MP, Satish S, Raveesha KA. Phytochemical analysis and antibacterial activity of Oxalis corniculata; a known medicinal plant. My Sci 2006;1:72-8.

Pradeepa M, Kalidas V, Geetha N. Qualitative and quantitative phytochemical analysis and bactericidal activity of pelargonium graveolens L’HER. Int J Appl Pharm 2016;8:7-11.

Daferera DJ, Ziogas BN, Polissiou MG. The effectiveness of plant essential oils in the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis. Crop Prot 2003;22:39–44.

Aboaba OO, Efuwape BM. Antibacterial properties of some Nigerian species. Biol Res Commum 2001;13:183–8.

Published

2016-10-01

How to Cite

Panda, E., C. Pradhan, and A. B. Das. “VARIATIONS IN PHYTOCONSTITUENTS AND ANTIMICROBIAL ACTIVITIES IN ECOTYPES OF OXALIS CORNICULATA L. AND OXALIS DEBILIS KUNTH”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 10, Oct. 2016, pp. 270-5, doi:10.22159/ijpps.2016v8i10.14069.

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