BERRY EXTRACTS IMPROVED INFLAMMATORY CYTOKINES, ANTIOXIDANT ENZYME AND SUPPRESSED THE GENE EXPRESSION ALTERATIONS IN DIABETIC RATS
Objective: The present work was conducted to evaluate the ameliorative effect of red berry (Morus rubra) and white berry (Morus alba) extracts in diabetic rats.
Methods: Inflammatory biomarkers; tumor necrosis factor-Î± (TNF-Î±), interleukin-1Î² (IL-1Î²), transforming growth factor-Î² (TGF-Î²), paraoxanase1 (PON1), gene expression of apoptosis and inflammatory related genes were carried out.
Results: Treatment of male rats with streptozotocin (STZ) to induce diabetes increased the levels of TNF-Î±, IL-1Î², TGF-Î², the apoptosis rates and the alterations of the pro-apoptosis (eotaxin, caspase-1, and caspase-2) and inflammation; nuclear factor kappa-light-chain-enhancer of activated B cells (NF-ÎºB1) and allograft inflammatory factor 1 (AIF-1) related genes. While marked reduction in PON1 level was detected in STZ-induced diabetic rats. However, treatment of STZ-exposed rats with red and white berry extracts exhibited noticeable ameliorations in TNF-Î±, IL-1Î², TGF-Î² levels and PON1 activity as well as low apoptosis rates and decreased the alteration of the pro-apoptosis and inflammatory related genes.
Conclusion: Red and white berry extracts exhibited attenuation in antioxidant status, suppressed inflammatory cytokines, apoptosis and the alterations in the apoptosis and inflammatory related genes induced by STZ in male rats.
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