ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE

  • Ahmed R. Abu-raghif Al-Nahrain University/College of Medicine/ Pharmacology Department, Baghdad-Iraq
  • Ghaith A. Jasim Al-Mustansiriyah University/College of Pharmacy/Department of Pharmacology & Toxicology, Baghdad-Iraq
  • Muneer M. Hanoon Thi Qar Health Office/ Thi Qar Poison Control Center

Abstract

Objective: The aim of this study was to investigate the capability of Zizyphus spina christi methanol extract to inhibit cancer cell line proliferation.

Methods: The leaves of Zizyphus spina christi were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay.

Results: The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC50 was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC50 of 33.91 mg/ml.

Conclusion: Methanol extract showed potential anti-proliferative activity against RD cell line, which could be due to its antioxidant activity.

Keywords: Anti-proliferative activity, Zizyphus spina christi, Methanol extract, RD cell line, MTT assay, DPPH assay

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Abu-raghif, A. R., G. A. Jasim, and M. M. Hanoon. “ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 2, Feb. 2017, pp. 279-82, doi:10.22159/ijpps.2017v9i2.15013.
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