PHYTOCHEMICAL ANALYSIS, ANTIMICROBIAL ACTIVITY AND ASSESSMENT OF POTENTIAL COMPOUNDS BY THIN LAYER CHROMATOGRAPHY OF ETHANOL FRACTION OF ASPARAGUS RACEMOSUS ROOTS

Authors

  • SUCHISMITA ROY Research Unit, Developed by Department of Nutrition, Physiology & Microbiology, Raja N. L. Khan Women's College, Midnapore, West Bengal, India
  • Shrabani Pradhan Research Unit, Developed by Department of Nutrition, Physiology & Microbiology, Raja N. L. Khan Women's College, Midnapore, West Bengal, India
  • Shreya Mandal Research Unit, Developed by Department of Nutrition, Physiology & Microbiology, Raja N. L. Khan Women's College, Midnapore, West Bengal, India
  • Koushik Das Research Unit, Developed by Department of Nutrition, Physiology & Microbiology, Raja N. L. Khan Women's College, Midnapore, West Bengal, India
  • Arpita Patra Research Unit, Developed by Department of Nutrition, Physiology & Microbiology, Raja N. L. Khan Women's College, Midnapore, West Bengal, India
  • Animesh Samanta Research Unit, Developed by Department of Nutrition, Physiology & Microbiology, Raja N. L. Khan Women's College, Midnapore, West Bengal, India
  • Banadeb Sinha Dept. of Biochemistry, Midnapore Medical College and Hospital, Midnapore, West Bengal, India
  • Sanjoy Kar Dept. of Botany, Midnapore College, Midnapore, West Bengal, India
  • Dilip Kumar Nandi Research Unit, Developed by Department of Nutrition, Physiology & Microbiology, Raja N. L. Khan Women's College, Midnapore, West Bengal, India

Keywords:

Asparagus Racemosus, Phytochemical, Antimicrobial, Antioxidant, Flavonoid

Abstract

Objectives: Asparagus racemosus (AR) Willd, is commonly known as Shatavari, belongs to family Liliaceae, and has potent medicinal values. The present study deals with detail Pharmacological evaluation of ethanol fraction of AR.

Methods: Preliminary phytochemical screening, antimicrobial and antioxidant activity, qualitative analysis of antioxidant and flavonoid detection, total phenol and flavonoid content were measured.

Results: Phytochemical screening showed the presence of various phytoconstituents like phytosterols, phenols, flavonoids, saponins and carbohydrates. Ethanol fraction of Asparagus racemosus roots have maximum relative percentage inhibition against gram (-) bacteria. The root displayed significant DPPH free radical scavenging activity against standard ascorbic acid. Thin layer chromatography of the ethanol fraction was also performed to determine the active principles. Four major spots obtained with the Rf values 0.20, 0.22, 0.24 and 0.58. Total flavonoid and phenol content was 188.3 mg/g quercetin equivalent and 165.22mg/g gallic acid equivalent respectively.

Conclusion: The findings of the current study shows that Asparagus racemosus is a good source of various phytochemicals and can be useful to progress and surge further scientific investigation to prevent and management of different diseases by their medicinal properties.

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Author Biography

SUCHISMITA ROY, Research Unit, Developed by Department of Nutrition, Physiology & Microbiology, Raja N. L. Khan Women's College, Midnapore, West Bengal, India

Associate professor and corresponding author

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Published

31-08-2014

How to Cite

ROY, S., S. Pradhan, S. Mandal, K. Das, A. Patra, A. Samanta, B. Sinha, S. Kar, and D. K. Nandi. “PHYTOCHEMICAL ANALYSIS, ANTIMICROBIAL ACTIVITY AND ASSESSMENT OF POTENTIAL COMPOUNDS BY THIN LAYER CHROMATOGRAPHY OF ETHANOL FRACTION OF ASPARAGUS RACEMOSUS ROOTS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 367-70, https://innovareacademics.in/journals/index.php/ijpps/article/view/1520.

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