EFFICACY OF MICRODOSES POTENTIZED HOMEOPATHIC DRUG, ARSENICUM ALBUM INDUCED BY ARSENIC TRIOXIDE IN ZEBRAFISH (DANIO RERIO)-A FTIR STUDY IN GILLS


Vijay Velu, Ramesh Uthandakalaipandian

Abstract


Objective: Arsenic, one of the major natural as well as anthropogenic pollutant produces biochemical changes in the organs of animals due to its potential toxicity. Arsenic is toxic to fish and may be taken through gills and skin. In the current work, arsenic trioxide-treated fishes were prescribed with potentized homoeopathic drug, ars alb 30C, and ars alb 200C. The biochemical contents in exposed gill tissues of zebrafish (Danio rerio) were analysed using Fourier transform infrared spectroscopy (FTIR).

Methods: The acclimatised fish were treated with arsenic trioxide and LC50 was calculated. Along with arsenic trioxide, the homoeopathic drug ars alb 30C and ars alb 200C were given three drops per litre. After a period of 7 and 14 d, the gills of exposed fish were dissected. The samples were lyophilized and analysed in FTIR.

Results: In arsenic exposed fish, the amine N-H stretch was different when compared to the control and the treated different potencies of ars alb 30C and 200C respectively at 7days. Whereas at 14 d the arsenic exposed fish the amine N-H stretch was drastically reduced. Notably, in treating different potencies of ars alb 30C and 200C, the amine N-H stretch was increased.

Conclusion: It was found that the higher the potency that is ars alb 200C was more effective against Arsenic toxicity. It shows higher dilution may be efficacious.


Keywords


Arsenic trioxide, Ars alb, Gills, FTIR

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About this article

Title

EFFICACY OF MICRODOSES POTENTIZED HOMEOPATHIC DRUG, ARSENICUM ALBUM INDUCED BY ARSENIC TRIOXIDE IN ZEBRAFISH (DANIO RERIO)-A FTIR STUDY IN GILLS

Keywords

Arsenic trioxide, Ars alb, Gills, FTIR

DOI

10.22159/ijpps.2017v9i1.15314

Date

31-12-2016

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 1, 2017 Page: 48-51

Online ISSN

0975-1491

Statistics

53 Views | 44 Downloads

Authors & Affiliations

Vijay Velu
Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu

Ramesh Uthandakalaipandian
Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu


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