ANTIDIABETIC ACTIVITY OF LINALOOL AND LIMONENE IN STREPTOZOTOCIN- INDUCED DIABETIC RAT: A COMBINATORIAL THERAPY APPROACH.
Keywords:Linalool, Limonene, Glucose uptake, Invitro protein glycation, Diabetes mellitus, Combinatorial therapy
Objective: To demonstrate the efficacy of a protein glycation inhibitor (limonene) and antihyperglycemic plant component (linalool) in combination towards better management of diabetes
Methods: Antihyperglycemic activity was assessed by OGTT and glucose uptake studies on diaphragm tissue. Protein glycation inhibition was studied using BSA as a model protein through fructosamine measurement. In vivo effect of mono and combinatorial therapy was assessed through oral treatment on streptozotocin induced diabetic rats
Results: Linalool at 3mM demonstrated 1.3mg/g tissue glucose uptake in 30 min as against 1.5 mg/g uptake by 2 unit insulin. In combination with insulin it showed an uptake of 2.2 mg /g tissue. Limonene demonstrated 85.61 % inhibition of protein glycation at 100 ÂµM while the positive control aminoguanidine demonstrated 88.02% inhibition at 1mM concentration. In vivo studies has demonstrated lowering of blood glucose, improved lipid profile and glycated hemoglobin. Combinatorial therapy demonstrated significantly improved kidney parameters viz. urine glucose, albumin and creatinine levels as compared to diabetic control rats.
Conclusion: Combination of an antihyperglycemic agent with a protein glycation inhibitor is more efficient in control of diabetes.
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