ROLE OF SUSPENDED PARTICULATE MATTER IN ANGIOGENESIS EMPLOYING CROWN GALL TUMOR ASSAY


Simerpreet Kaur, Jatinder K. Katnoria

Abstract


Objective: Aim of the study was to screen the suspended particulate matter for their role in enhancing angiogenesis employing crown gall tumor assay.

Methods: Particulate matter (PM10) samples were collected, on glass fiber filter paper at the rate of 1.12–1.14 m3/min for 8 hr using high volume sampler, from six different sites of Amritsar city. Aqueous extracts of suspended particulate matter were prepared using a mechanical shaker for 24 hour and the filtrate was centrifuged and lyophilized.

Results: Maximum (34.67±1.764) tumors were induced by samples collected from Crystal chowk whereas tumor inducing ratio (TIR) was found to be comparably high for two sites viz., Crystal chowk and Bhandari bridge.

Conclusion: High tumor inducing ratio at the above-mentioned sites was correlated to high traffic emission from automobile exhaust. Crown gall tumor assay has been proved to be rapid, economical and reliable screening assay for angiogenesis agent.


Keywords


Particulate matter, Carcinogenicity, Tumor-inducing ratio

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About this article

Title

ROLE OF SUSPENDED PARTICULATE MATTER IN ANGIOGENESIS EMPLOYING CROWN GALL TUMOR ASSAY

Keywords

Particulate matter, Carcinogenicity, Tumor-inducing ratio

DOI

10.22159/ijpps.2017v9i1.15440

Date

31-12-2016

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 1, 2017 Page: 34-37

Online ISSN

0975-1491

Statistics

127 Views | 154 Downloads

Authors & Affiliations

Simerpreet Kaur
Department of Botanical and Environmental Sciences, Guru Nanak Dev Universtiy, Amritsar 143005, Punjab (India)
India

Jatinder K. Katnoria
Department of Botanical and Environmental Sciences, Guru Nanak Dev Universtiy, Amritsar 143005, Punjab (India)
India


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