• K. Sasikala Department of Chemistry, Saranathan College of Engineering, Tiruchirappalli 620012, Tamil Nadu, India
  • S. CHANDRA MOHAN Research and Development Centre, Bharathiar University, Coimbatore -641 046, Tamil Nadu, India.


Objective: In this study methanol extract of leaves of canthium coromandelicum (Family: Rubiaceae) was screened for the presence of phytochemical components by GC-MS analysis. In addition total phenolics and flavanoids were also estimated.

Methods: GC-MS analysis of plant extract was performed using a Perkin-Elmer GC Clarus 500 system and Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST).

Result: 20 chemical compounds are identified from the plant extract. Squalene (C30H50) is the major component available at RT 23.10 and 15.02% peak area. Other most prevailing phytocomponds are Phytol, 25-desacetoxy- Cucurbitacin b, n-Hexadecanoic acid, Sitosterol etc., Canthium coromandelicum contained the total phenolic of 25.49±0.09 mg/g and the total flavonoids of 12.37±0.15mg/g which are equivalent to gallic acid and quercetin, respectively.

Conclusion: The presence of various bioactive compounds justifies the use of canthium coromandelicum for various ailments by traditional practitioners.

Keywords: Canthium coromandelicum, GC-MS, Phytochemical screening, Methanol extract


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1. Mathekaga AD, Meyer JJM. Antibacterial activity of South African Helichrysum species. South Afr J Bot 1998;64:293–5.
2. De Fatima A, Modolo L V, Conegero L, S Pilli R A, Ferreira CV, Kohn Lk et al. Styryl Lactones and Their Derivatives:Biological Activities, Mechanisms of Action and Potential Leads for Drug Design. J Curr Med Chem 2006;13:3371-84.
3. Chandra Mohan S, Sasikala K, Anand T, Vengaiah PC, Krishnaraj S. Green synthesis, Antimicrobial and Antioxidants Effects of Silver Nanoparticles using Canthium coromandelicum Leaves Extract. R J Microbiol 2014;9:142-50.
4. Shankar S, Thiripura Salini S. Evaluation of in vitro Antioxidant Activity of Canthium coromandelicum. R J Med Plant 2014;8:149-55.
5. Chandra Mohan S, Sasikala K,Anand T.Antimicrobial and Wound Healing Potential of Canthium coromandelicum Leaf Extract-A Preliminary Study. R J Phytochem 2014;8:35-41.
6. Bray HG, Thorpe WV:Analysis of Phenolic compounds of Interest in Metabolism. J Meth Biochem Anal 1954;1:27-52.
7. Chandra Mohan S, Balamurugan V, Elayaraja R, Prabakaran AS. Antioxidant and phytochemical potential of medicinal plant kalonchoe pinnata. Int J Pharm Sci Res 2012;3:881-5.
8. Kohno Y, Egawa Y, Itoh S, Nagaoka S, Takahashi M, Mukai K. Kinetic study of quenching reaction of singlet oxygen and scavenging reaction of free radical by squalene in n-butanol. J Biochim Biophys Acta 1995;1256:52-6.
9. Huang Z R, Lin YK, Fang JY. Biological and pharmacological activities of squalene and related compounds:Potential uses in cosmetic dermatology. J Molecules 2009,14:540-54.
10. Fox CB. Squalene emulsions for parenteral vaccine and drug delivery. J Molecules 2009,14:3286-312.
11. Newmark HL. Squalene, olive oil, and cancer risk. Review and hypothesis. J Ann N Y Acad Sci 1999;889:193-203.
12. Rao CV, Newmark HL, Reddy BS. Chemopreventive effect of squalene on colon cancer. J Carcinogenesis 1998,19:287-90.
13. Trichopoulou A, Lagiou P, Kuper H, Trichopoulos D. Cancer and Mediterranean dietary traditions. J Cancer Epidemiol Biomarkers Prev 2000;9:869-73.
14. Yarkoni E, Rapp HJ. Tumor regression after intralesional injection of mycobacterial components emulsified in 2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene (squalene), 2,6,10,15, 19,23-hexamethyltetracosane (squalane), peanut oil, or mineral oil. J Cancer Res 1979;39:1518-20.
15. Pimm MV, Baldwin RW, Lederer E. Suppression of an ascitic rat hepatoma with cord factor and Nocardia cell wall skeleton in squalene emulsions. Eur J Cancer 1980;16:1645-47.
16. Nakagawa M, Yamaguchi T, Fukawa H, Ogata J, Komiyama S, Akiyama S, Kuwano, M. Potentiation by squalene of the cytotoxicity of anticancer agents against cultured mammalian cells and murine tumor. Jpn J Cancer Res 1985;76:315-20.
17. Anand T, Gokulakrishnan K. Phytochemical Analysis of Hybanthus enneaspermus using UV, FTIR and GC-MS. IOSR-PHR 2012;2:520-4.
18. Kondo T, Inoue M, Mizukami H, Ogihara Y. Cytotoxic activity of bryonolic acid isolated from transformed hairy roots of Trichosanthes kirilowii var. japonica. J Biol Pharm Bull 1995;18:726-9.
19. Duncan KLK, Duncan MD, Alley MC, Sausville EA. Cucurbitacin E-induced disruption of the actin and vimentin cytoskeleton in prostate carcinoma cells. J Biochem Pharmacol 1996;52:1553-60.
20. Chandravadana MV, Nidiry ESJ, Venkateshwarlu G. Antifungal activity of momordicines from Momordica charantia. J Fitoterapia 1997;68:383-4.
21. Agil A, Miró M, Jimenez J, Aneiros J, Caracuel MD, García-Granados A et al. Isolation of ani-hepatotoxic principle form the juice of Ecballium elaterium. J Planta Med 1999;65:673-5.
22. Peters RR, Saleh TF, Lora M, Patry C, de Brum-Fernandes AJ, Farias MR, Ribeiro-do-Valle RM. Anti-inflammatory effects of the products from Wilbrandia ebracteata on carrageenan induced pleurisy in mice. J Life Sci 1999;64:2429-37.
23. Zahir Hussain A, Aruna Ignatius. GC-MS Analysis and Antimicrobial Activity of Acalypha Indica Linn. J Asian J Chem 2010;22:3591-5.
24. Saravanan P, Chandra Mohan G, Maria Jancy Rani J, Shanmuga Sundaram P. GC-MS analysis of phytochemical constituents in ethanolic bark extract of ficus religiosa linn. Int J Pharm Pharm Sci 2014;6:457-60.
25. Akhlaghi M, Bandy B. Mechanisms of flavonoid protection against myocardial ischemia-reperfusion injury. J Mol Cell Cardial 2009;46:309-17.
26. Xu YJ, Kaur M, Dhillon RS, Tappia PS, Dhalla NS. Health benefits of sea buckthorn for the prevention of cardiovascular diseases. J Funt Foods 2011;3:2-12.
27. Nugroho AE, Abdul Malik, Suwidjiyo Pramono. Total phenolic and flavonoid contents, and in vitro antihypertension activity of purified extract of Indonesian cashew leaves (Anacardium occidentaleL.). Int Food Res J 2013;20:299-305.
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How to Cite
Sasikala, K., and S. CHANDRA MOHAN. “TOTAL PHENOLIC, FLAVANOID CONTENTS AND GC-MS ANALYSIS OF CANTHIUM COROMANDELICUM LEAVES EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 8, Aug. 2014, pp. 379-81,
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