MICROORGANISMS AS CHEMICAL FACTORIES FOR ISOLATION OF META-BOLOMES FROM MESOPHILIC SOIL
Keywords:Microorganisms, Chemical factories, Metabolomes, Mesophilic
Objective: To investigate the micro-flora of the mesophilic soil of Rajasthan for isolation of novel compounds having antibacterial potentials.
Methods: In present experiments, bacterial colonies were isolated from four different regions of Jaipur, Rajasthan and screened for antimicrobial efficacy against five selected pathogens Pseudomonas aeruginosa MTCC 7093, Staphylococcus aureus MTCC 7443, Escherichia coli MTCC 40, Klebsiella pneumoniae MTCC 530, and Bacillus subtillis MTCC 121. Antimicrobial efficacy against the selected strains was performed. The potential efficacy of the extract was also screened for gas chromatographyâ€“mass spectroscopy (GC-MS) analysis for novel metabolites screening. Further, potent bacterial strains were identified at the molecular level by 16S ribosomal deoxyribonucleic acid (DNA) sequencing method.
Results: After the primary screening, 29 microbial isolates were selected for the screening of bioactivity. Results displayed zones of inhibition ranging from 5 mm till maximum 13 mm. Soil testing indicated survival conditions for microbes isolated, and biochemical tests supported the identification of screened isolates. The potentially isolated strains S-III C, S-III D and S-IV D were identified at the molecular level using 16S ribosomal DNA sequencing as Bacillus shackletonii, Bacillus thuringiensis and Bacillus subtilis subsp. inaquosorum, respectively.
Conclusion: Extraction of active metabolites from soil microbiota, against five pathogenic bacteria, is far better, safe and economical method. This study will help in exploring new compounds against increasing number of resistant pathogenic strains with an aim to reduce demand of medicinal plants for extraction of effective antimicrobial compounds. Bacillus strains (S-IIIC, S-IIID and S-IVC) isolated from soil microflora possess antimicrobial activity and can be used for isolation of antibiotics at industrial levels.
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