MIMOSA PUDICA EXERTS NEUROPROTECTION AGAINST MPP+INDUCED NEUROTOXICITY IN SHSY5Y CELL LINES-AN IN VITRO MODEL OF ANTI-PARKINSONISM


Mahadevan M. V., Ramaswamy R. S., Banumathi V.

Abstract


Objective: Parkinson’s disease (PD) is one of the of a neurodegenerative disorder, It’s decreased the dopaminergic neurones, tyrosine hydroxylase (TH) and increased the α-synuclein protein level. This study was conducted to investigate the neuroprotective effect of Mimosa pudica have the abilities to improve TH and DAT proteins expression against MPP+ induced neurotoxicity, in in vitro model of Parkinson’s disease using SH-SY5Y human neuroblastoma cell lines.

Methods: Mimosa pudica were pre-treated with various concentration for cell viability assay. Vehicle alone or Mimosa pudica (300µg) for 24 h, and then were co-treated with 1000µM MPP+ for 15 min in the continued presence of vehicle or Mimosa pudica. After treatment, cells were collected for protein expression.

Results: Cell viability assay confers the inhibitory concentration cell death of Mimosa pudica. MPP+significantly down-regulated the protein expression of TH (p<0.01) and DAT (p<0.05). Mimosa pudica decreased the expression of α synuclein (p<0.01) in MPP+ intoxicated cell lines.

Conclusion: The present study showed that Mimosa pudica exerts neuroprotection by suppressing α synuclein and the dopaminergic neurdegeneration. Mimosa pudica may be due to quecertin which might be acted via the anti-oxidant mechanism. The above finding suggests that Mimosa pudica may act as a potential target in the management of PD.

 


Keywords


Parkinson’s disease, Mimosa pudica, Siddha medicine, SH-SY5Y, MPP+, α synuclein, Tyrosine hydroxylase, Dopamine Transporter

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About this article

Title

MIMOSA PUDICA EXERTS NEUROPROTECTION AGAINST MPP+INDUCED NEUROTOXICITY IN SHSY5Y CELL LINES-AN IN VITRO MODEL OF ANTI-PARKINSONISM

Keywords

Parkinson’s disease, Mimosa pudica, Siddha medicine, SH-SY5Y, MPP+, α synuclein, Tyrosine hydroxylase, Dopamine Transporter

DOI

10.22159/ijpps.2017v9i1.15728

Date

31-12-2016

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 1, 2017 Page: 21-26

Online ISSN

0975-1491

Statistics

139 Views | 129 Downloads

Authors & Affiliations

Mahadevan M. V.
Lecturer, Sirappu Maruthuvam Department, National Institute of Siddha, Chennai - 600047, India
India

Ramaswamy R. S.
Director General, Central Council for Research in Siddha, Ministry of AYUSH, Govt of India, Govt Anna Hospital campus, Arumbakkam, Chennai 600106, India
India

Banumathi V.
Director, National Institute of Siddha, Chennai 600047, India


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