• Doha H. Abou Baker Medicinal and Aromatic Plant Department, National Research Centre (NRC), 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, Egypt, P.O.12622
  • Eman A. Ibrahim Plant Biochemistry Department, National Research Centre (NRC), 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, Egypt, P.O.12622
  • Ahmed Kandeil Water Pollution Research Department, National Research Centre (NRC), 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, Egypt, P.O. 12622
  • Farouk K. El Baz Plant Biochemistry Department, National Research Centre (NRC), 33 El Bohouth st. (former El Tahrir st.), Dokki, Giza, Egypt, P.O.12622



Ruta graveolens L, Murraya paniculata L, phytosterols, bioactivity


Objective: Ruta graveolens L. (R. graveolens) and Murraya paniculata L. (M. paniculata) are medicinal plants belonging to Rutaceae family have many uses in traditional medicine. The aim of the present study was to investigate sterols bioactivity of the two Rutaceae plant leaves.

Methods: Sterols of the two Rutaceae plant leaves were identified using GC/MS. The antioxidant activities of the sterols of these herbs were evaluated by three different methods; free radical scavenging using 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) and total antioxidant activity. The anticancer activity of the sterols was determined by MTT assay against colorectal cancer HCT116, breast cancer MCF7, liver cancer HepG2 and lung cancer A549 cell lines. Anti-inflammatory activity was evaluated using albumin denaturation assay and antiviral activities against H5N1 virus were carried out using plaque reduction assay.

Results: GC/MS assay showed β-Sitosterol (36%) as the most abundant sterols of R. graveolens followed by stigmasterol (18%), while stigmasterol (25.2%) was the most abundant one of M. paniculata steroids. The anti-inflammatory potential of R. graveolens steroids was significantly higher than that of diclofenac sodium (standard drug). M. paniculata sterols have higher antiviral activity (IC50= 0.15 of µg/ml) than R. graveolens sterols (IC50= 7.8 of µg/ml). The sterols of R. graveolens showed anticancer activity against MCF7 and A549 cells with inhibition 84.3 and 81%, at 100 µg/ml respectively. While M. paniculata sterols showed 77.3% inhibition against A549 cells.

Conclusion: The current study suggests that the sterols of M. paniculata have more anti-viral activity than R. graveolens sterols which showed more anticancer and anti-inflammatory activities.


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Lavanya R, Maheshwari SU, Harish G, Raj JB, Kamali S, Hemamalani D, et al. Investigation of in vitro anti-inflammatory, antiplatelet and arthritic activities in the leaves of Anisomeles malabarica L. Res J Pharm Biol Chem Sci 2010;1:745-52.

Harsha SN, Latha BV. In vitro antioxidant and in vitro anti-inflammatory activity of Ruta graveolens methanol extract. Asian J Pharm Clin Res 2011;5:32-5.

Ratheesh M, Shyni GL, Sindhu G, Helen A. Protective effects of isolated polyphenolic and alkaloid fractions of Ruta graveolens L. on acute and chronic models of inflammation. Inflamm 2010;33:18-24.

Van Huyssteen M, Milne PJ, Campbell EE, van de Venter M. Antidiabetic and cytotoxicity screening of five medicinal plants used by traditional African health practitioners in the Nelson Mandela Metropole, South Africa. Afr J Tradit Complementary Altern Med 2011;â€8:150-8.

Meepagala KM, Schrader K, Wedge DE, Duke OS. Algicidal and antifungal compounds from the roots of Ruta graveolens and synthesis of their analogs. Phytochemicals 2005;66:2689-95.

Khouri NA, El-Akawi Z. Antiandrogenic activity of Ruta graveolens L in male Albino rats with emphasis on sexual and aggressive behavior. Neuroendocrinol Lett 2005;26:823-9.

Chávez M, Franco I, Tlatenco GM. Tradition and Herbal Remedies. Mexico City: Ce-Ãcatl, AC; 2003. p. 80-1.

Browner CH. Plants used for reproductive health in Oaxaca, Mexico. Econ Bot 1985;39:482-504.

Atta AH, Alkofahi A. Antinociceptive and anti-inflammatory effects of some Jordanian medicinal plant extracts. J Ethnopharmacol 1998;60:117-24.

Mancebo F, Hilje L, Mora GA. The biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae. Rev Biol Trop 2001;49:501-8.

Sallal AJ, Alkofahi A. Inhibition of the hemolytic activities of snake and scorpion venoms invitro with plant extracts. Biomed Lett 1996;53:211-5.

Barbosa FS, Leite GLD, Alves SM, Nascimento AF, D'Ãvila VA, Costa CA. Insecticide effects of Ruta graveolens, Copaifera langsdorffii and Chenopodium ambrosioides against pests and natural enemies in commercial tomato plantation. Maringa 2011;33:37-43.

Junghanns KT, Kneusel RE, Groger D, Matern U. Differential regulation and distribution of acridone synthase in Ruta graveolens. Phytochemicals 1998;49:403-11.

SLTC, The Society of Leather Traders Chemists Official methods of Analysis; 1963.

Arnao MB, Cano A, Acosta M. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem 2001;73:239-44.

Ye ZW, Jiang JG, Wu GH. Biosynthesis and regulation of carotenoids in Dunaliella: progresses and prospects. Biotechnol Adv 2008;26:352-60.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phospho- molybdenum complex: specific application to the determination of Vitamin E. Anal Biochem 1999;269:337-40.

Rahman H, Eswaraiah MC, Dutta AM. In vitro anti-inflammatory and anti-arthritic activity of Oryza sativa Var. Joha Rice (An Aromatic Indigenous Rice of Assam). Am-Eurasian J Agric Environ Sci 2015;15:115-21.

Rashad AE, Shamroukh AH, Abdel-Megeid RE, Mostafa A, El-Shesheny R, Kandeil A, et al. Synthesis and screening of some novel fused thiophene and thienopyridine derivatives for anti-avian influenza virus (H5N1) activity. Eur J Med Chem 2010;45:5251-7.

Hayden FG, Cote KM, Douglas RG. Plaque inhibition assays for drug susceptibility testing of influenza viruses. Antimicrob Agents Chemother 1980;17:865-70.

Mosmann T. Rapid colorimetric assays for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.

Thabrew MI, Hughes RD, McFarlane IG. Screening of hepatoprotective plant components using a HepG2 cell cytotoxicity assay. J Pharm Pharmacol 1997;49:1132-5.

El-Menshawi BS, Fayad W, Mahmoud K, El-Hallouty SM, El-Manawaty M, Olofsson MH, et al. Screening of natural products for therapeutic activity against solid tumors. Indian J Exp Biol 2010;48:258-64.

Matthausa B, Özcanb MM. Chemical evaluation of citrus seeds, an agro-industrial wastes a new potential source of vegetable oil. Grasas Aceites 2012;63:313-20.

Woyengo TA, Ramprasath VR, Jones PJH. Anticancer effects of phytosterols. Eur J Clin Nutr 2009;63:813-20.

Raju MP, Babu DG, Kumar BR, Rajashekar CH. The role of phytosterols enriched foods-a review. J Environ Sci Toxicol Food Technol 2013;7:40-7.

Halliwell B. Antioxidants in human health and disease. Ann Rev Nutr 1996;16:33-50.

Valantina RS, Neelamegam P. Selective ABTS and DPPH-radical scavenging activity of peroxide from vegetable oils. Int Food Res J 2014;22:289-94.

Gautam MK, Gupta A, Rao CV, Goel RK. Antihyperglycemic and antioxidant potential of Murraya paniculata linn. leaves: a preclinical study. J Pharm Res 2012;5:1334-7.

Rodríguez EJ, Ramis-Ramos G, Heyden YV, Simó-Alfonso EF, Lerma-García MJ, Saucedo-Hernández Y, et al. Chemical composition, antioxidant properties and antimicrobial activity of the essential oil of Murraya paniculata leaves from the mountains of Central Cuba. Nat Prod Commun 2012;7:1527-30.

Mita TA, Shihan MH, Rahman M, Sharmin T, Maleque M, Alvi MR, et al. In vitro antioxidant, cytotoxic, thrombolytic, antimicrobial and membrane stabilising activities of Murraya paniculata. Am J Res Commun 2013;1:226-37.

Chen CH, Chan HC, Chu YT, Ho HY, Chen PY, Lee TH, et al. Antioxidant activity of some plant extracts towards xanthine oxidase, lipoxygenase and tyrosinase. Molecules 2009;14:2947-58.

Haraguchi H. Antioxidative plant constituents. In: Tingali C. Ed. Bioactive compounds from natural sources. New York: Taylor and Francis Inc; 2001. p. 339-77.

Harsha SN, Latha BV. In vitro antioxidant and in vitro anti-inflammatory activity of Ruta Graveolens methanol extract. Asian J Pharma Clin Res 2012;5:32-5.

Gupta NB, Hath R, Srivastava N, Shanker K, Kishor K, Bhargava KP. Anti-inflammatory and antipyretic activities of β-sitosterol. Planta Med 1980;39:157-63.

Prieto JM, Recio MC, Giner RM. Anti-inflammatory activity of β-sitosterol in a model of oxazolone-induced contact-delayed-type hypersensitivity. Bol Latinoam Caribe Plant Med Aromat 2006;5:57-62.

Laidlaw M. Pilot study conducted at The University of Guelph, by the human nutraceutical research unit on the supplement Sterol 117â„¢; 2005.

Mayer AMS. Therapeutic implications of microglia activation by lipopolysaccharide and reactive oxygen species generation in septic shock and central nervous system pathologies: a review. Medicina (Buenos Aires) 1998;58:377-85.

Malini T, Vanithakumari G. Rat toxicity studies with β-sitosterol. J Ethnopharmacol 1990;28:221-34.

Khan MM, Jain DC, Bhakuni RS, Zaim M, Thakur RS. The occurrence of some antiviral sterols in Artemisia annua. Plant Sci 1991;75:161-5.

Wachsman MB, López MF, Ramirez JA, Galagovsky LR, Coto CE. Antiviral effect of brassinosteroids against herpes virus and arenaviruses. Antiviral Chem Chemother 2000;11:71-7.

Wachsman MB, Ramirez JA, Galagovsky LR, Coto C. Antiviral activity of brassinosteroids derivatives against measles virus in cell cultures. Antiviral Chem Chemother 2002;13:61-6.

Fadella K, Watson A, Yehualaeshet T, Turner T, Samuel T. Ruta Graveolensextract induces DNA damage pathways and blocks akt activation to inhibit cancer cell proliferation and survival. Anticancer Res 2011;31:233-41.

Choi J, Lee E, Lee H, Kim K, Ahn K, Shim B, et al. Identification of campesterol from Chrysanthemum coronarium L and its antiangiogenic activities. Phytother Res 2007;21:954-9.

Lopes G, Sousa C, Valentao P, Andrade PB. Sterols in algae and health. Plant Animal Sources 2013;10:7-15.

De Stefani E, Boffetta P, Ronco AL, Brennan P, Deneo-Pellegrini H, Carzoglio JC, et al. Plant sterols and risk of stomach cancer: a case-control study in uruguay. Nutr Cancer 2000;37:140-4.

Mendilaharsu M, de Stefani E, Deneo-Pellegrini H, Carzoglio J, Ronco A. Phytosterols and risk of lung cancer: a case-control study in Uruguay. Lung Cancer 1998;21:37-45.

McCann SE, Freudenheim JL, Marshall JR, Graham S. Risk of human ovarian cancer is related to dietary intake of selected nutrients, phytochemicals and food groups. J Nutr 2003;133:1937-42.

Ju YH, Clausen LM, Allred KF, Almada AL, Helferich WG. β-Sitosterol, β-sitosterol glucoside, and a mixture of β-sitosterol and b-sitosterol glucoside modulate the growth of estrogen responsive breast cancer cells in vitro and ovariectomized athymic mice. J Nutr 2004;134:1145-51.

Bradford PG, Awad AB. Modulation of signal transduction in cancer cells by phytosterols. Biofactors 2010;36:241-7.

Meric J, Rottey S, Olaussen K, Soria J, Khayat D, Rixe O, et al. Cyclooxygenase-2 as a target for anticancer drug development. Crit Rev Oncol Hematol 2006;59:51-64.

The American Institute for Cancer Research (AICR). Plant Compound Continue to Challenge Science. AICR; Washington, DC, USA; 2006.

Chai JW, Kuppusamy UR, Kanthimathi MS. Beta-sitosterol induces apoptosis in MCF-7 cells. Malays J Biochem Mol Biol 2008;16:28-30.

Awad AB, Downie AC, Fink CS. Inhibition of growth and stimulation of apoptosis by beta-sitosterol treatment of MDA-MB-231 human breast cancer cells in culture. Int J Mol Med 2000;5:541-6.

Park C, Moon DO, Rhu CH, Choi BT, Lee WH, Kim GY, et al. β-sitosterol induces antiproliferation and apoptosis in human leukemic U937 cells through activation of caspase-3 and induction of Bax/Bcl-2 ratio. Biol Pharm Bull 2007;30:1317-23.



How to Cite

Baker, D. H. A., E. A. Ibrahim, A. Kandeil, and F. K. E. Baz. “ AND MURRAYA PANICULATA L”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 2, Feb. 2017, pp. 103-8, doi:10.22159/ijpps.2017v9i2.15790.



Original Article(s)