A STUDY ON THE BIOACTIVE POTENTIAL OF FRESH AND DRIED SPROUTS OF COCOS NUCIFERA L.–AN IN VITRO AND IN-SILICO APPROACH


Abiraami Valli S., S. Uma Gowrie

Abstract


Objective: The main objective of the study is to analyze the medicinal properties, by giving scientific validation to the existing bioactive compounds present in the coconut sprouts and recommending the coconut sprouts as a natural product for the treatment of bacterial infection leading to inflammations.

Methods: Phytochemical screening by qualitative and quantitative analysis of the primary and secondary constituents were carried out in aqueous and methanol extracts of the coconut sprouts (fresh and dried) using standard procedures. The phytochemicals were characterized using FT-IR, TLC and GC-MS analysis. Antibacterial activity was studied against several human pathogens by agar well diffusion method. Antioxidant activity using Reducing power, Hydrogen peroxide scavenging and DPPH radical scavenging methods and Anti-inflammatory activity through protein denaturation method were carried out. Further confirmation of the functional role of the phytoconstituent through in-silico docking was studied.

Results: The qualitative phytochemical screening revealed the presence of essential phytoconstituents. The quantitative analysis revealed the presence of maximum Carbohydrates (0.60±0.1 mg/g), Proteins (39±0.8 mg/ml), Flavonoids (0.28±0.1 mg QE/g) and Terpenoids (95±1.2 mg/g) in methanolic fresh coconut sprout extract with respective standards. Maximum zone of inhibitions were shown by Shigella flexneri, Salmonella typhi and Klebsiella pneumoniae. Antioxidant and anti-inflammatory studies proved the presence of therapeutic bioactive potential in the sample. Through in-silico analysis, docking studies were performed to confirm the functional role of the specific therapeutic phytochemical.

Conclusion: The fresh coconut sprouts are natural, economically potent food source for human health and can be a nutrient supplement with cost effective approach. The dried sprouts can also be recommended to the food industry for the large-scale production of nutrient-based foods with a quality check.


Keywords


Coconut sprouts, Phytochemical, Characterization, Antioxidant, Anti-inflammatory, In silico

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References


AACR, Broccoli sprouts, cabbage, Ginkgo biloba and garlic: a grocery list for cancer prevention, Am Asso Cancer Research. Public and Media: News; 2005.

Schenker S. Facts behind the headlines, Broccoli, British Nutrition Foundation-Nutrition Bulletin; 2002;27:159-60.

Finley JW. Proposed criteria for assessing the efficacy of cancer reduction by plant foods enriched in carotenoids, glucosinolates, polyphenols and seleno-compounds. Ann Bot 2005;95:1075-96.

Webb GP. Dietary supplements and functional foods, Blackwell Publishing Ltd., Oxford; 2006. p. 1-120.

Rufus Auxilia L, Rachel Regi Daniel, Shenbagarathai R. Phytochemical analysis of seed extracts Macrotyloma uniflorum (Horse gram). Int J Curr Res 2013;5:3339-42.

EFSA, Scientific Opinion on the risk posed by Shiga-toxin-producing Escherichia coli (STEC) and other pathogenic bacteria in seeds and sprouted seeds. European Food Safety Authority; 2011.

Lima EBC, CNS Sousa, LN Meneses, NC Ximenes, GS Vasconcelos, NBC Lima, et al. Cocos nucifera (L.) (Arecaceae): a phytochemical and pharmacological review. Braz J Med Biol Res 2015;48:953–64.

Anitha Madhusoodhanan Chikku, Thankappan Rajamohan. Dietary coconut sprout beneficially modulates cardiac damage induced by isoproterenol in rats. Bangladesh J Pharmacol 2012;7:258-65.

Bandyopadhyay D, Biswas K, Bhattacharyya M, Reiter RJ, Banerjee RK. Gastric toxicity and mucosal ulceration induced by oxygen-derived reactive species, protection by melatonin. Curr Mol Med 2001;1:501-13.

Ludovico B, Eric S, Van DV. Helicobacter pylori cytotoxin-associated gene A subverts the apoptosis-stimulating protein of p53 ASPP2 tumor suppressor pathway of the host. Proc Natl Acad Sci USA 2011;108:9238-43.

Martin JB. Not all Helicobacter pylori strains are created equal, should all be eliminated. Lancet 1997;349:1020-22.

Fan XG, Kelleher D, Fan XJ, Xia HX, Keeling PW. Helicobacter pylori increase proliferation of gastric epithelial cells. Gut 1996;38:19-22.

Akram M, Shahabuddin, Ahmed A, Usmanghani K, Hannan A, Mohiuddin E. Peptic ulcer and Helicobacter pylori eradication. Int J Med Sci 2010;2:370-5.

Leslie. Peptic ulcer: a reappraisal of its peptic aetiology. Ann R Coll Surg Engl 1972;50:145-63.

Nagar V, Bandekar JR. Microbiological quality of packaged sprouts from supermarkets in Mumbai, India. Int J Food Safety Nutr Public Health 2009;2:165-75.

Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol 1998;60:1-8.

Sofowora AO. Medicinal plants and traditional medicine in Africa. 2nd ed. Sunshine house, Ibadan, Nigeria: Spectrum books Ltd., Screening plants for Bioactive agents; 1993. p. 134-56.

Harborne AJ. Phytochemical methods-a guide to modern techniques of plant Analysis, Thomas Science Publications; 1998.

Raaman N. Phytochemical Analysis, New India Publishing Agency; 2006. p. 19-24.

Trease GE, Evans WC. Pharmacognosy. 15th ed. London, Saunders publishers; 2002. p. 42-4.

Miller GL. Use of dinitro salicylic acid reagent for determination of reducing sugar. Anal Chem 1972;31:426-8.

Nelson N. A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 1944;153:375-80.

Lowry OH, NJ Rosebrough, AL Fan, RJ Randall. Protein measurement with the Folin-phenol reagent. J Biol Chem 1951;193:265-75.

Patel S, Patel J, Patel RK. To study proximate analysis and biological evaluation of Triphala Guggulu formulation. Int J PharmTech Res 2012;4:1520-6.

Pallab K, Tapan B, Tapas P, Ramen K. Estimation of total flavonoids content (TPC) and antioxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. J Drug Delivery Ther 2013;3:33-7.

Ferguson N. A Textbook of Pharmacognosy, Max Millam Company; 1956. p. 191.

Wheeler EL, RE Ferrel. A method for phytic acid determination in wheat and wheat fractions. Cereal Chem 1971;48:312-20.

Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharma Pharmacol 1968;20:169-73.

Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci 2010;2:146-55.

Ruch RT, Cheng SJ, Klaunig JE. Spin trapping of superoxide and hydroxyl radicals. Methods Enzymol 1984;105:198-209.

Oyaizu M. Studies on the product of browning reaction prepared from glucose amine. Jap J Nutr 1986;44:307-15.

Mansoor Ahmad, Farah Saeed, Mehjabeen, Noor Jahan. Evaluation of the insecticidal and antioxidant activity of selected medicinal plants. J Pharmacogn Phytochem 2013;2:153-8.

Patel Rajesh M, Patel Natvar J. In vitro antioxidant activity of coumarin compounds by DPPH, superoxide and nitric oxide free radical scavenging methods. J Adv Pharm Edu Res 2011;1:52-68.

Achola KJ, RW Munenge. Bronchodilating and uterine activities of Ageratum conyzoides extract”. Pharm Biol 1998;36:93-6.

Mamta Arora, Satnam Singh, Ramandeep Kaur. Phytochemical analysis, protein content and antimicrobial activities of selected samples of Glycine Max Linn. Int J Res Eng Technol 2013;2:570-4.

Aun Syed, Nighat Fatima. In silico analysis and molecular docking studies of potential angiotensin converting enzyme inhibitor using quercetin glycosides. Pharmacognosy Mag 2015;11(Suppl 1):S123–S126.

Elena Penas, Rosario Gomeza, Juana Frias, Vidal­Valverde. Application of high-pressure treatment on Alfalfa (Medicago sativa) and Mung bean (Vigna radiata) seeds to enhance the microbiological safety of their sprouts. Food Control 2008;19:698-705.

McGarvey DJ, Croteau R. Terpenoid metabolism. Plant Cell 1995;7:1015-26.

Indumathi, Durgadevi, Nithyavani, Gayathri. Estimation of terpenoid content and its antimicrobial property in Enicostemma litorrale. Int J ChemTech Res Coden (USA) 2014;6:4264-7.

Ravi Kumar P, G Shalini, M Jeyam. Phytochemical analysis and antioxidant activity of wheat grains and seedlings. J Pharm Sci Innov 2014;3:319-23.

Caterina Brajdes, Camelia Vizireanu. Sprouted buckwheat an important vegetable source of antioxidants. Food Technol 2012;36:53-60.

Ashima Bali, Satyanarayana T. Advances in microbial biotechnology, APH Publishing Corporation, New Delhi, India; 2009. p. 94-8.

Reddy NR. Occurrence, distribution, content, and dietary intake of phytate. In: Reddy NR, Sathe SK. Eds. Food Phytates. CRC Press; Boca Raton Florida: 2002. p. 25–51.

Skoog A, EJ Holler, SR Crouch. Principles of instrumental analysis. 6th ed. 2007. p. 1039.

Blakeslee K, Penner KP. Microorganisms and food-borne illness, Kansas State University Publication; 2006.

Stermitz FR, Tawara-Matsuda J, Lorenz P, Mueller P, Zenewicz L, Lewis. K, 5′-methoxyhydnocarpin-D and pheophorbide A: berberis species components that potentiate berberine growth inhibition of resistant Staphylococcus aureus. J Nat Prod 2000;63:1146-9.

Randhir R, Shetty K. Mung bean processed by solid-state bioconversion improves phenolic content and functionality relevant for diabetes and ulcer management. Innov Food Sci Emerg Technol 2007;8:197-204.

Smid EJ, Gorris LGM. Natural antimicrobials for food preservation. In: Rahman MS. Ed. Handbook of Food Preservation. Marcel Dekker, New York; 1999. p. 285-308.

Leelaprakash G, Mohan Dass S. In the vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. Int J Drug Dev Res 2011;3:185-96.

Mitchell RN, Cotran RS. Acute and chronic inflammation. In: Robbins Basic Pathology Saunders, Philadelphia, USA; 2003;7:33-59.

Lewis. Anti-inflammatory drugs from plants and marine sources. Agents Actions Suppl 1989;27:3-373.

Cotran RS, Kumar V, Robbins SL. Pathologic basis of disease, Saunders WB company, Phildelphia, USA; 1994.

Antony De Paula Barbosa. An overview on the biological and pharmacological activities of saponins. Int J Pharm Pharma Sci 2014;6:0975-1491.

Suprava Sahoo, Goutam Ghosh, Debajyoti Das, Sanghamitra Nayak. Phytochemical investigation and in vitro antioxidant of an indigenous medicinal plant Alpinia nigra B. L. Burtt. Asian Pac J Trop Biomed 2013;3:871–6.

Saeed N, RK Muhammad, S Maria. Antioxidant activity, total phenolic and flavonoid contents of the whole plant extract Torilis leptophylla L. BMC Complementary Altern Med 2012;12:1-12.

Nilsson J, Stegmark R, Akesson B. Total antioxidant capacity in different pea (Pisum sativum) varieties after blanching and freezing, Food Chem 2004;86:501-7.

Halliwell B. Gutteridge, Oxygen toxicity, oxygen radicals, transition metals and diseases. Biochem J 1984;219:1-4.

Niki E, Shimaski H, Mino H. Antioxidantism-free radical and biological defense, Gakkai Syuppan Center, Tokyo; 1994. p. 3-16.

Imaida K, Fukushima S, Shivai T, Ohtani M, Nakanishi K, Ito N. Promoting activities of butylated hydroxyl anisole and butylated hydroxyl toluene on 2-stage urinary bladder caricinogensis and inhibition of γ-glutamyl transpeptidase-positive foci development in the liver of rats. 1983;4:885-9.

Singsaas EL, Lerdau M, Winter K, Sharkey TD. Isoprene increases thermotolerance of isoprene-emitting species. Plant Physiol 1997;115:1413-20.

Crowell PL. Terpenes in breast cancer prevention. Breast Cancer 1997;46:191-7.

Banani Dass, Manabendra Dutta Choudhury, Amitabha Dey, Anupam Das Talukdar, KH Nongallemia, Lokesh Deb. Antioxidant and anti-inflammatory activity of aqueous and methanolic extracts of rhizome part of drynaria quercifolia (L.) J. smith. Int J Pharm Pharm Sci 2014;6:43-9.

Nassar Z, Aisha A, Abdul Majid A. The pharmacological properties of terpenoids from Sandoricum Koetjape, webmed central; 2010. p. 2-11.

Sharma DK. Pharmacological properties of flavonoids including flavonolignans-Integration of petro crops with drug development in plants. J Sci Indus Res 2006;65:477-84.

Syed Zameer Hussain, Khushnuma Maqbool. GC-MS: principle, technique and its application in food science. Int J Curr Sci 2014;13:116-26.




About this article

Title

A STUDY ON THE BIOACTIVE POTENTIAL OF FRESH AND DRIED SPROUTS OF COCOS NUCIFERA L.–AN IN VITRO AND IN-SILICO APPROACH

Keywords

Coconut sprouts, Phytochemical, Characterization, Antioxidant, Anti-inflammatory, In silico

DOI

10.22159/ijpps.2017v9i3.16014

Date

03-02-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 3, 2017 Page: 129-142

Online ISSN

0975-1491

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117 Views | 626 Downloads

Authors & Affiliations

Abiraami Valli S.
Department of Plant Biology and Plant Biotechnology, Ethiraj College for Women, Chennai 600008, Tamil Nadu, India
India

S. Uma Gowrie
Department of Plant Biology and Plant Biotechnology, Ethiraj College for Women, Chennai 600008, Tamil Nadu, India
India


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