PHYTOCHEMICAL STUDIES AND GC-MS ANALYSIS OF SPERMADICTYON SUAVEOLENS ROXB


Ruthiran Papitha, Lokesh Ravi, Chinnadurai Immanuel Selvaraj

Abstract


Objective: The present study was performed to identify the phytochemical constituents of leaves and flowers of a plant Spermadictyon suaveolens extracted with four different solvents.

Methods: Dried and powdered samples were subjected to soxhlation based on the polarity of the solvents. The extracts were scanned using Ultra Violet-visible (UV-Vis) spectrophotometry with the wavelength ranging from 200–800 nm by comparing the absorption spectrum with the spectra of known compounds, Fourier Transform Infrared (FT-IR) spectrometry was used to find out the functional groups of the compounds and GC-MS system consisting of a Perkin Elmer Technologies Model Clarus 680 GC equipped with Clarus 600 (EI) was used to identify the metabolites by matching their recorded mass spectra with the standard mass spectra from National Institute of Standards and Technology (NIST05. LIB) libraries provided by the software of the GCMS system (TurboMass version 5.4.2).

Results: The phytochemical tests indicated the presence of carbohydrates, alkaloids, flavonoids, phenols, tannins, saponins and terpenoids from the chloroform extract of leaves and flowers. UV-visible spectrophotometer results indicated a wavelength range between 230–660 nm for the flower and leaf extracts for major peaks. FT-IR analysis indicated major functional groups such as aromatic, primary, secondary and aliphatic amines, alkanes, carboxylic acids and amides. GC-MS analysis results revealed major bioactive compounds in the crude extracts.

Conclusion: Presence of secondary metabolites has been identified from the phytochemical studies. Many phyto-compounds have been identified from the leaves and flowers of using GC-MS analysis. Hence, this medicinal plant may be used as a source for treating many diseases.


Keywords


Medicinal plant, Phytochemical screening, UV-visible spectrophotometry, FTIR, GC-MS

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About this article

Title

PHYTOCHEMICAL STUDIES AND GC-MS ANALYSIS OF SPERMADICTYON SUAVEOLENS ROXB

Keywords

Medicinal plant, Phytochemical screening, UV-visible spectrophotometry, FTIR, GC-MS

DOI

10.22159/ijpps.2017v9i3.16059

Date

03-02-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 3, 2017 Page: 143-149

Online ISSN

0975-1491

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183 Views | 1047 Downloads

Authors & Affiliations

Ruthiran Papitha
Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology University, Vellore, India
India

Lokesh Ravi
Department of Biomedical Science, School of Biosciences and Technology, Vellore Institute of Technology University, Vellore, India

Chinnadurai Immanuel Selvaraj
Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology University, Vellore, India
India


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