Ruthiran Papitha, Lokesh Ravi, Chinnadurai Immanuel Selvaraj


Objective: The present study was performed to identify the phytochemical constituents of leaves and flowers of a plant Spermadictyon suaveolens extracted with four different solvents.

Methods: Dried and powdered samples were subjected to soxhlation based on the polarity of the solvents. The extracts were scanned using Ultra Violet-visible (UV-Vis) spectrophotometry with the wavelength ranging from 200–800 nm by comparing the absorption spectrum with the spectra of known compounds, Fourier Transform Infrared (FT-IR) spectrometry was used to find out the functional groups of the compounds and GC-MS system consisting of a Perkin Elmer Technologies Model Clarus 680 GC equipped with Clarus 600 (EI) was used to identify the metabolites by matching their recorded mass spectra with the standard mass spectra from National Institute of Standards and Technology (NIST05. LIB) libraries provided by the software of the GCMS system (TurboMass version 5.4.2).

Results: The phytochemical tests indicated the presence of carbohydrates, alkaloids, flavonoids, phenols, tannins, saponins and terpenoids from the chloroform extract of leaves and flowers. UV-visible spectrophotometer results indicated a wavelength range between 230–660 nm for the flower and leaf extracts for major peaks. FT-IR analysis indicated major functional groups such as aromatic, primary, secondary and aliphatic amines, alkanes, carboxylic acids and amides. GC-MS analysis results revealed major bioactive compounds in the crude extracts.

Conclusion: Presence of secondary metabolites has been identified from the phytochemical studies. Many phyto-compounds have been identified from the leaves and flowers of using GC-MS analysis. Hence, this medicinal plant may be used as a source for treating many diseases.


Medicinal plant, Phytochemical screening, UV-visible spectrophotometry, FTIR, GC-MS

| PDF | HTML |


Vijyalakshmi R, Ravindran R. Preliminary comparative phyto-chemical screening of root extracts of Diospyros ferrea (Wild.) Bakh and Arvalanata (L.)Juss. Ex Schultes. Asian J Plant Sci Res 2012;2:581-7.

Gupta MK, Shrivastava AK, Singh AP, Chauhan, Gaur DS, Mishra KK, et al. Comparative study of the shiva guggulu and simhanada guggulu in the management of amavata (rheumato-id arthritis). Int J Appl Pharm 2016;2:5-12.

Mahato SB, Sen S. Analysis of oil composition of the methanol extract of Coriandrum sativum Linn fruit by soxhlation tech-nique. Int J Curr Pharm Res 2014;4:53-4.

Herouart D. Variations in the leaf alkaloid content of androgenic diploid plants of Datura innoxia. Planta Medica 1988;54:14-7.

Singh NP, Karthikeyan S. Flora of Maharashtra State Dicotyle-don. Vol. 1. Flora of India. Series-2." Botanical Survey of India, Calcutta; 2000. p. 783.

Singh P, Ali SJ. Ethnomedicinal plants of family rubiaceae of Eastern UP. Indian J Life Sci 2012;1:83-6.

Patil D. Ethnobotany of Buldhana district (Maharashtra: India): Plants used in veterinary medicine. J Phytol 2011;2:22-34.

Kavita M, Rakshe A, Mokat D. Pharmacognostic studies of drug spermadictyon suaveolens roxb. Int J Pharm 2016;3:234-9.

Gaur R. Flora of the district Garhwal, North-West Himalaya (with ethnobotanical notes) Srinagar: Transmedia xii, 811p. ISBN 8190080733 En Anatomy and morphology Keys. Geog; 1999. p. 6.

Jackman R, Smith J. Anthocyanins and betalains, in Natural food colorants; 1996. p. 244-309.

Rice-Evans C, Miller N. Antioxidant activities of flavonoids as bioactive components of food. Biochem Soc Trans 1996;24:790-5.

Poiyamozhi A, Sundaraganesan N, Karabacak M, Tanrıverdi O, Kurt M. The spectroscopic (FTIR, FT-Raman, UV and NMR), first-order hyperpolarizability and HOMO–LUMO analysis of 4-amino-5-chloro-2-methoxybenzoic acid. J Mol Struc 2012;1024:1-12.

Coates J. Interpretation of infrared spectra, a practical ap-proach. Encyl of Anal Chem; 2000. p. 1-23.

Tsunoda A. Antiviral activity of alpha-methyl-1-adamantane-methylamine hydrochloride. Anti-Micro Agents C 1965;5:553.

Zhu ZZ. Analgesic, anti-inflammatory and antipyretic activities of the petroleum ether fraction from the ethanol extract of Desmodium podocarpum. J Ethnopharmacol 2011;133:1126-31.

Dangoggo S. Phytochemical analysis and antibacterial screen-ing of leaves of Diospyrosme spiliformis and Ziziphusspina Christi. J Chem Eng 2012;1:57-62.

Silva RO. Phytol, a diterpene alcohol, inhibits the inflammatory response by reducing cytokine production and oxidative stress. Fund Clin Pharmacol 2014;28:455-64.

Padilla SS, Grizzle TB, Lyerly D. Triphenylphosphite: in vivo and in-vitro inhibition of rat neurotoxic esterase. Toxicol Appl Pharmacol 1987;87:249-6.

Al-Wathnani H. Bioactivity of natural compounds isolated from cyanobacteria and green algae against human pathogenic bacteria and yeast. J Med Plants Res 2012;6:3425-33.

Dahpour AA, Rahdari P, Sobati Z. The chemical composition of essential oil, antibacterial activity and brine shrimp lethality of ethanol extracts from Sedum pallidum. J Med Plants Res 2012;6:3105-9.

Govindappa M. Chemical composition of methanol extract of endophytic fungi, Alternaria sp. of Tebebuia argentea and their antimicrobial and antioxidant activity. Int J Life Sci Biotechnol Pharma Res 2014;5:861-9.

Sadananda T, Govindappa M, Ramachandra Y. In vitro antioxi-dant activity of lectin from different endophytic fungi of Viscum album L. Br J Pharm Res 2014;4:626.

Patil U, Deshmukh O. GC-MS Analysis of phytochemicals in the aqueous extract of Cyclea peltata.(Lam) Hook. f. and Thomson. Int J Sci Res 2016;4:350-1.

Abubacker MN, Palaniyappan KD. In vitro antifungal potentials of bioactive compounds heptadecane, 9-hexyl and ethyl iso-allocholate isolated from Lepida gathiscristata Wild (Acanthaceae) leaf. Br Biomed Bull 2015;3:336-43.

Nakamura K, Hatakeyama T, Hatakeyama H. DSC Studies on hydrogen bonding of poly (4-hydroxy-3, 5-dimethoxystyrene) and related derivatives. Polym J 1986;18:219-25.

Igwe OU, Okwu DE. GC-MS Evaluation of bioactive compounds and antibacterial activity of the oil fraction from the seeds of Brachystegia eurycoma (HARMS). Asian J Plant Sci Res 2013;3:47-54.

Sathyaprabha G. A comparative study on antioxidant, prox-imate analysis, antimicrobial activity and phytochemical analysis of Aloe vera and Cissus quadrangularis by GC-MS. J Pharm Res 2010;3:2970-3.

Ertas A, Yilmaz MA, Firat M. Chemical profile by LC–MS/MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two Euphorbia species. Nat Prod Res 2015;29:529-34.

Yinusa I, George NI, Ayo RG, Rufai Y. Evaluation of the pharma-cological activities of beta-sitosterol isolated from the bark of Sarcocephalus latifolius (Smith Bruce). Globle J Pure Appl Chem Res 2015;3:7-14.

About this article




Medicinal plant, Phytochemical screening, UV-visible spectrophotometry, FTIR, GC-MS





Additional Links

Manuscript Submission


International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 3, 2017 Page: 143-149

Online ISSN



183 Views | 1047 Downloads

Authors & Affiliations

Ruthiran Papitha
Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology University, Vellore, India

Lokesh Ravi
Department of Biomedical Science, School of Biosciences and Technology, Vellore Institute of Technology University, Vellore, India

Chinnadurai Immanuel Selvaraj
Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology University, Vellore, India

Article Tools


  • There are currently no refbacks.