ANTIOXIDANT AND ANTICANCER ACTIVITY OF METHANOLIC EXTRACT FROM STEPHANIA ELEGANS
Objective: The present work was aimed to investigate the in vitro antioxidant and anti-cancer activities of methanolic extract of Stephania elegans, an unexplored species from Menispermaceae family.
Methods: The methanolic extract of S. elegans tubers was prepared and phytochemical screening and total phenolic content were analyzed by using standard methods. In vitro, antioxidant potential of methanolic extract was determined by 2-2â€™-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays. Cytotoxicity against human breast cancer cell line, Michigan Cancer Foundation-7 type (MCF-7) was evaluated by 3-(4, 5 dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) (MTT) assay.
Results: Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, carbohydrates, tannins, terpenoids, steroids, and saponins in the methanolic extract of S. elegans tubers. The total phenolic content in the methanolic extract was 23.0Â±0.06 mg GAE/g (dry mass). As revealed by ABTS assay, the methanolic extract of plant tubers showed significant radical scavenging activity (IC50 41.66Â±0.015 Î¼g/ml). The reducing power activity of the extract increased with the concentration of the extract. MTT assay indicated that S. elegans has potent cytotoxic activity towards MCF-7 cells (IC50 158.7Â±0.13 Î¼g/ml).
Conclusion: This is the first study demonstrating the antioxidant and anticancer capabilities of the methanolic extract of S. elegans. This study also provides a significant basis for further isolation and characterization of bioactive compounds from S. elegans.
2. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin 2011;61:69â€“90.
3. WHO. Global Status Report on Non-communicable Diseases. Geneva, Switzerland; 2011.
4. Ali Seyed M, Jantan I, Vijayaraghavan K, Bukhari SN. Betulinic acid: recent advances in chemical modifications, effective delivery and molecular mechanisms of a promising anticancer therapy. Chem Biol Drug Des 2015;87:517-36.
5. Ngugi M Piero, Njagi M Joan. Cancer: a molecular curse? Int J Curr Pharm Res 2015;7:1-3.
6. Aggarwal BB, Van Kuiken ME, Iyer LH, Harikumar KB, Sung B. Molecular targets of nutraceuticals derived from dietary spices: potential role in suppression of inflammation and tumorigenesis. Exper Biol Med 2009;234:825-49.
7. Costa-Lotufo LV, Khan MTH, Ather A, Wilke DV, Jimenez PC, Pessoa C, et al. Studies of the anticancer potential of plants used in Bangladeshi folk medicine. J Ethnopharmacol 2005;99:21â€“30.
8. Fouche G, Cragg GM, Pillay P, Kolesnikova N, Maharaj VJ, Senabe J. In vitro anticancer screening of South African plants. J Ethnopharmacol 2008;119:455â€“61.
9. Kamatou GPP, Van Zyl RL, Davids H, Van Heerden FR, Lourens ACU, Viljoen AM. Antimalarial and anticancer activities of selected South African Salvia species and isolated compounds from S. radula. S Afr J Bot 2008;74:238â€“43.
10. Y Cai, Q Luo, M Sun, Corke. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 2004;74:2157â€“84.
11. Willcox J, Sarah A, Catignani G. Antioxidants and prevention of chronic disease. Crit Rev Food Sci Nutr 2004;44:275â€“95.
12. Sireesha K, Sailaja Rao P. Oxidative stress and diabetes: an overview. Asian J Pharm Clin Res 2015;8:15-9.
13. Klaunig JE, Kamendulis LM. The role of oxidative stress in carcinogenesis. Ann Rev Pharmacol Toxicol 2004;44:239â€“67.
14. Dizdaroglu M, Jaruga P, Birincioglu M, Rodriguez H. Free-radical-induced damage to DNA: mechanisms and measurement. Free Radical Biol Med 2002;32:1102â€“15.
15. Glasauer A, Chandel NS. Targeting antioxidants for cancer therapy. Biochem Pharmacol 2014;92:90-101.
16. Seeram NP, Adams LS, Zhang Y, Lee R, Sand D, Scheuller HS, et al. Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro. J Agric Food Chem 2006;54:9329-39.
17. Zhang Y, Seeram NP, Lee R, Feng L, Heber D. Isolation and identification of strawberry phenolics with antioxidant and human cancer cell antiproliferative properties. J Agric Food Chem 2008;56:670-5.
18. Deepak Kumar Semwal, Ruchi Badoni, Ravindra Semwal, Sudhir Kumar Kothiyal, Gur Jas Preet Singh, Usha Rawat. The genus Stephania (Menispermaceae): chemical and pharmacological perspectives. J Ethnopharmacol 2010;132:369â€“83.
19. Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed. Allahabad: L. M. Basu; 2004. p. 94.
20. Gaur RD. Flora of District Garhwal North West Himalaya. 1st ed. Srinagar Garhwal, India: Trans Media; 1999. p. 76â€“7.
21. Chopra RN, Chopra IC, Handa KL, Kapur LD Chopra. Indigenous Drugs of India. 2nd ed. Calcutta, India: Char UN and Sons Ltd; 1958. p. 412.
22. Hong De Xu, Soon-Chang Cho, Mi-Ae Bang, Chun-Sik Bae, Yeon Shik Choi, Yong-Chun Li, et al. FK-3000 isolated from Stephania delavayi Diels. Inhibits MDA-MB-231 cell proliferation by decreasing NF-ÎºB phosphorylation and COX-2 expression. Int J Oncol 2015;46:2309-16.
23. Moongkarndi P, Kosem N, Luanratana O, Jongsomboonkusol S, Pongpan N. Antiproliferative activity of thai medicinal plant extracts on human breast adenocarcinoma cell line. Fitoterapia 2004;75:375â€“7.
24. Nakaoji K, Nayeshiro H, Tanahashi T, Su Y, Nagakura N. Bisbenzylisoquinoline alkaloid from Stephania cepharantha and their effects on proliferation of cultured cell from the Murine hair apparatus. Planta Med 1997;63:425â€“8.
25. Montririttigri K, Moongkarndi P, Joongsomboonkusol S, Chitkul B, Pattanapanyasat K. Apoptotic activity of aporphine from Stephania venosa on human ovarian cancer cells. Mahidol Univ J Pharm Sci 2008;35:52â€“6.
26. Yang DL, Mei WL, Dai HF. Cytotoxic alkaloids from the tuber of Stephania succifera. Chin J Med Chem 2010;20:206â€“10.
27. Singh RS, Kumar P, Bhakuni DS. The Alkaloids of Stephania elegans. J Nat Prod 1981;44:664â€“7.
28. Sofowara A. Medicinal plants and traditional medicine in Africa. Ibadan, Nigeria: Spectrum Books Ltd; 1993. p. 191-289.
29. Trease GE, Evans WC. Pharmacognosy. 11th ed. London: Bailliere Tindall; 1989. p. 45-50.
30. Harborne JB. Phytochemical methods. 2nd ed. London, NewYork: Chapman and Hall publications; 1984. p. 288.
31. Singleton VL, Orthofer R, Lamuela Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymol 1999;299:152â€“78.
32. Kriengsak Thaipong, Unaroj Boonprakob, Kevin Crosby, Luis Cisneros-Zevallos, David Hawkins Byrne. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Comp Anal 2006;19:669â€“75.
33. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 1996;239:70-6.
34. Scudiero DA, Shoemaker RH, Paull KD, Monks A, Tierney S, Nofziger TH, et al. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 1988;48:4827â€“33.
35. B Tepe, M Sokmen, HA Akpulat, A Sokmen. Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem 2006;95:200-4.
36. W Zheng, SY Wang. Antioxidant activity and phenolic compound in selected herbs. J Agric Food Chem 2001;49:5165â€“70.
37. Fadila Maiza Benabdesselam, Sabiha Khentache, Khalida Bougoffa, Mohamed Chibane, Sandrine Adach, Yves Chapeleur, et al. Antioxidant activities of alkaloid extracts of two Algerian species of Fumaria; Fumaria capreolata and Fumaria bastardii. Rec Nat Prod 2007;1:2-3,28-35.
38. Soon Huat Tiong, Chung Yeng Looi, Hazrina Hazni, Aditya Arya, Mohammad javad Paydar, Won Fen Wong, et al. Antidiabetic and antioxidant properties of Alkaloids from Catharanthus roseus (L.) G. Don. Molecules 2013;18:9770-84.
39. Lokesh Ravi, Manasvi V, Praveena Lakshmi B. Antibacterial and antioxidant activity of saponin from Abutilon Indicum leaves. Asian J Pharm Clin Res 2016;9:1-4.
40. Mayura A Kale, Suparna M Bindu, Pratima Khadkikar. Role of antioxidants and nutrition in oxidative stress: a review. Int J Appl Pharm 2015;7:1-4.
41. Singh Lubhan, Najmi Abul Kalam, Sara Udai Veer Singh, Majhi Sagarika. In vitro antioxidant activity of ethanolic extract of Stephania Glabra (Roxb) mier tubers. Pharma Res 2014;12:1-11.
42. Sharma U, Sahu RK, Roy A, Golwala DK. In vivo antidiabetic and antioxidant potential of Stephania hernandifolia in streptozotocin-induced-diabetic rats. J Young Pharm 2010;2:255-60.
43. SY Chor, AY Hui, KF To, KK Chan, YY Go, HLY Chan, et al. Anti-proliferative and pro-apoptotic effects of herbal medicine on the hepatic stellate cell. J Ethnopharmacol 2005;100:180â€“6.
44. Leewanich P, Worachartcheewan A, Prachayasittikul S, Prachayasittikul V. Anticancer and antioxidative activies of Stephania venosa. Eur J Sci Res 2011;51:150â€“6.
45. Tomofumi Uto, Masaaki Toyama, Keisuke Yoshinaga, Masanori Baba. Cepharanthine induces apoptosis through the mitochondria/caspase pathway in murine dendritic cells. Immunopharm Immunot 2016;38:238-43.
46. Meng LH, Zhang H, Hayward L, Takemura H, Shao RG, Pommier Y. Tetrandrine induces early. G1 arrest in human colon carcinoma cells by down-regulating the activity and inducing the degradation of G1-S-specific cyclin-dependent kinases and by inducing p53 and p21Cip1. Cancer Res 2004;64:9086-92.
47. Rathinam Prema, Dhana Sekaran Sathish Sekar, Kothapalli Bannoth Chandra Sekhar, Somasundaram Jeevanandham. In vitro cytotoxicity study on combined plants extracts (Cissus quadrangularis and Aegle marmelos). Eur J Exp Biol 2012;2:882-8.
48. Matsuo M, Sasaki N, Saga K, Kaneko T. Cytotoxicity of flavonoids toward cultured normal human cells. Biol Pharm Bull 2005;28:253-9.
49. Neerja Gupta, Saxena G, Kalra SS. Antimicrobial activity pattern of certain terpenoids. Int J Pharm Biol Sci 2011;1:87-91.
50. Birt DB, Hendrich S, Wang W. Dietary agents in cancer prevention: Flavonoids and isoflavonoids. Pharmacol Thera 2001;90:157-77.
51. Mullick T, Gasser E. Nutrition in the chemoprevention of gastrointestinal cancer: Where are we in the new millennium. Nutrition issues in the gastroenterology. Pract Gastroenterol 2004;15:52-64.
52. Zulak K, Liscombe D, Ashihara H, Facchini P. Alkaloids. Plant secondary metabolism in diet and human health. Oxford: Blackwell Publishing; 2006. p. 102â€“36.