EFFECT OF PRENATAL ADMINISTRATION OF THERAPEUTIC DOSE OF TOPIRAMATE ON PLACENTAE ALBINO RATS' FETUSES


Eman Y. Salah El-din, Amel R. Omar

Abstract


Objective: Topiramate is an antiepileptic drug (AED) used for the treatment of partial seizure in adult and children epileptic patients. It passed through the placenta causing a birth defect. However, little literature focused on placental alteration due to the administration of topiramate during pregnancy. So, applying different predictive parameters; placental weight, histopathological (Haematoxylin and Eosin), histochemical (periodic acid Schiff’s) and immunohistochemistry (Caspase-3).

Methods: Topiramate was orally administrated to the pregnant rats with dose 100 mg/kg rats from 5th to 19th day of gestation. The dam’s undergone hysterectomy and the placentae were weighted and stained by Haematoxylin and Eosin, periodic acid Schiff’s and Caspase-3.

Results: The study indicated that there is statistically significant (P<0.05) increase in the treated placental weight (0.4717±0.03788) compared with control group (0.5208±0.02930). Also, the light microscopic examination of the placental specimens using Haematoxylin and Eosin staining revealed that an alteration in both basal and labyrinth zone. Apoptotic feature in spongiotrophoblast and trophoblasts is detected. Positive Periodic acid Schiff’s reaction for polysaccharides in the topiramate-treated group. Caspase-3 is showing apoptotic cells in the trophoblast layer.

Conclusion: Long-term daily use of topiramate during pregnancy can lead to obvious pathological histotoxic effects in layers of placenta tissues which may be implicated in cognitive affection. Various effects of topiramate necessitate further investigations.


Keywords


Caspase-3, Periodic acid Schiff’s, Placenta, Topiramate

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About this article

Title

EFFECT OF PRENATAL ADMINISTRATION OF THERAPEUTIC DOSE OF TOPIRAMATE ON PLACENTAE ALBINO RATS' FETUSES

Keywords

Caspase-3, Periodic acid Schiff’s, Placenta, Topiramate

DOI

10.22159/ijpps.2017v9i3.16208

Date

03-02-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 3, 2017 Page: 54-59

Online ISSN

0975-1491

Statistics

80 Views | 481 Downloads

Authors & Affiliations

Eman Y. Salah El-din
Department of Zoology, Faculty of Science, Cairo University, Egypt
Egypt

Amel R. Omar
Department of Zoology, Faculty of Science, Cairo University, Egypt
Egypt


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