BIOASSAY-GUIDED EVALUATION OF FICUS SEMICORDATA FOR ANTIDIABETIC ACTIVITY


Virender Kaur, Kumud Upadhyaya, Milind Pande

Abstract


Objective: The early stage of diabetes mellitus type 2 is associated with postprandial hyperglycemia. The therapeutic approach involved in the treatment of type 2 diabetes mellitus is the use of agents that can decrease postprandial hyperglycemia by inhibiting carbohydrate digesting enzymes. In an effort of identifying herbal drugs which may become useful in the prevention or mitigation of diabetes, the antidiabetic activity of Ficus semicordata (FS) and its constituents were studied. The present study was undertaken in part to identify the potent antihyperglycemic fraction from the ethanol extract of the plant, using bioassay guided evaluation.

Methods: The ethanol extract of Ficus semicordata were fractionated to obtain chloroform, ethyl acetate, n-butanol and ethanol extracts which were tested for alpha-amylase, alpha-glucosidase inhibitory, properties. Further fractionation of the more active ethanol fraction yielded isolates FS-1 and FS-2 which were tested for in vivo antidiabetic activity using Streptozotocin (STZ)-induced diabetic rats.

Results: Ethanol extract from leaves of the plant showed notable alpha-amylase (IC50 = 3.352µg/ml and alpha-glycosidase inhibitory activity (IC50= 3.448µg/ml) as compared to standard acarbose (IC50 = 3.175µg/ml. Subfraction FS-1 and FS-2 which were tested for in vivo antidiabetic activity using acute STZ-induced diabetic rats significantly (*p<0.05, **p<0.01, *** p<0.001) reduced blood glucose level.

Conclusion: The Ficus semicordata plant extracts and the fractionated components could be used as a natural antidiabetic after comprehensive in vitro and in vivo biological studies.


Keywords


Ficus semicordata, Bioassay-guided evaluation, Alpha-amylase, Alpha-glucosidase inhibitory, Postprandial hyperglycemia, and STZ-induced diabetes

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About this article

Title

BIOASSAY-GUIDED EVALUATION OF FICUS SEMICORDATA FOR ANTIDIABETIC ACTIVITY

Keywords

Ficus semicordata, Bioassay-guided evaluation, Alpha-amylase, Alpha-glucosidase inhibitory, Postprandial hyperglycemia, and STZ-induced diabetes

DOI

10.22159/ijpps.2017v9i3.16441

Date

03-02-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 3, 2017 Page: 71-77

Online ISSN

0975-1491

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176 Views | 116 Downloads

Authors & Affiliations

Virender Kaur
NIMS Institute of Pharmacy, NIMS University, Shobha Nagar, Jaipur- Delhi Highway, Jaipur, Rajasthan, India, 303121
India

Kumud Upadhyaya
Department of Pharmaceutical Sciences, Kumaun University, Bhimtal Campus, Nainital, Uttarakhand, India, 263136
India

Milind Pande
NIMS Institute of Pharmacy, NIMS University, Shobha Nagar, Jaipur- Delhi Highway, Jaipur, Rajasthan, India, 303121
India


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