• Dokka Narasimham Department of Biotechnology, University of Calicut, Kerala, India
  • Yeduguri Hima Bindu Department of Biotechnology & Bioinformatics, Yogi Vemana University, A.P, India
  • Sanith Cheriyamundath Department of Biotechnology, University of Calicut, Kerala, India
  • Rahul Raghavan Department of Biotechnology, University of Calicut, Kerala, India
  • Meruva Keerthi Kumari Department of Environmental Science, Yogi Vemana University, A.P, India
  • Thummala Chandrasekhar Department of Environmental Science, Yogi Vemana University, A.P, India
  • Joseph Madassery Department of Biotechnology, University of Calicut, Kerala, India


Objective: To evaluate the anticancer and antioxidant activity of medicinal plant Clidemia hirta extracted in different solvents.

Methods: Crude extracts were prepared from the leaves of Clidemia hirta using ethanol, petroleum ether and chloroform solvents. Anticancer activities and antioxidant properties were assayed using standard yellow dye 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging assay respectively.

Results: We found that the ethanol extract had higher inhibition activities against Dalton's lymphoma ascites (DLA) cancer cell line, 50% DLA cell line inhibition at 68µg/ml, while 50% inhibition by petroleum ether and chloroform extracts were at 160µg/ml and 172µg/ml, respectively. The antioxidant activity requires5µg/ml of ethanol extract to trap 50% of DPPH (IC50), whereas the positive control ascorbic acid trapped 50% of DPPH (IC50) at 3.5µg/ml.

Conclusion: The prepared leaf extracts with different solvents of Clidemia hirta showed the antiproliferative and antioxidant activity in dose-dependent manner. Further works is required to identify the biologically active chemical constituents, responsible for cancer cell growth inhibition from this plant.

Keywords: Anticancer, Antioxidant, MTT Assay, DPPH Free Radical


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How to Cite
Narasimham, D., Y. H. Bindu, S. Cheriyamundath, R. Raghavan, M. K. Kumari, T. Chandrasekhar, and J. Madassery. “EVALUATION OF IN VITRO ANTICANCER AND ANTIOXIDANT ACTIVITIES FROM LEAF EXTRACTS OF MEDICINAL PLANT CLIDEMIA HIRTA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 9, no. 4, Feb. 2017, pp. 149-53, doi:10.22159/ijpps.2017v9i4.16843.
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