EVALUATION OF IN VITRO ANTICANCER AND ANTIOXIDANT ACTIVITIES FROM LEAF EXTRACTS OF MEDICINAL PLANT CLIDEMIA HIRTA


Dokka Narasimham, Yeduguri Hima Bindu, Sanith Cheriyamundath, Rahul Raghavan, Meruva Keerthi Kumari, Thummala Chandrasekhar, Joseph Madassery

Abstract


Objective: To evaluate the anticancer and antioxidant activity of medicinal plant Clidemia hirta extracted in different solvents.

Methods: Crude extracts were prepared from the leaves of Clidemia hirta using ethanol, petroleum ether and chloroform solvents. Anticancer activities and antioxidant properties were assayed using standard yellow dye 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging assay respectively.

Results: We found that the ethanol extract had higher inhibition activities against Dalton’s lymphoma ascites (DLA) cancer cell line, 50% DLA cell line inhibition at 68µg/ml, while 50% inhibition by petroleum ether and chloroform extracts were at 160µg/ml and 172µg/ml, respectively. The antioxidant activity requires5µg/ml of ethanol extract to trap 50% of DPPH (IC50), whereas the positive control ascorbic acid trapped 50% of DPPH (IC50) at 3.5µg/ml.

Conclusion: The prepared leaf extracts with different solvents of Clidemia hirta showed the antiproliferative and antioxidant activity in dose-dependent manner. Further works is required to identify the biologically active chemical constituents, responsible for cancer cell growth inhibition from this plant.


Keywords


Anticancer, Antioxidant, MTT Assay, DPPH Free Radical

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About this article

Title

EVALUATION OF IN VITRO ANTICANCER AND ANTIOXIDANT ACTIVITIES FROM LEAF EXTRACTS OF MEDICINAL PLANT CLIDEMIA HIRTA

Keywords

Anticancer, Antioxidant, MTT Assay, DPPH Free Radical

DOI

10.22159/ijpps.2017v9i4.16843

Date

27-02-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 4, 2017 Page: 149-153

Online ISSN

0975-1491

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120 Views | 52 Downloads

Authors & Affiliations

Dokka Narasimham
Department of Biotechnology, University of Calicut, Kerala, India
India

Yeduguri Hima Bindu
Department of Biotechnology & Bioinformatics, Yogi Vemana University, A.P, India

Sanith Cheriyamundath
Department of Biotechnology, University of Calicut, Kerala, India

Rahul Raghavan
Department of Biotechnology, University of Calicut, Kerala, India

Meruva Keerthi Kumari
Department of Environmental Science, Yogi Vemana University, A.P, India

Thummala Chandrasekhar
Department of Environmental Science, Yogi Vemana University, A.P, India

Joseph Madassery
Department of Biotechnology, University of Calicut, Kerala, India


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