COMPARATIVE ANALGESIC STUDIES OF LEAF AND STEM BARK OF CALOPHYLLUM INOPHYLLUM IN SWISS ALBINO MICE
Objectives: The aim of the present study was to perform acute oral toxicity studies and to compare and evaluate the analgesic effect of ethanolic extracts of leaf and stem bark of Calophyllum inophyllum on Swiss albino mice.
Methods: Eddyâ€™s hotplate method and acetic acid induced writhing were implemented to determine analgesic properties of the extracts. Both the extracts were administered orally. Acute oral toxicity studies were conducted using the OECD guidelines 423 Annexure â€“ 2d.
Results: The results indicate the mortality was not observed during the acute oral toxicity studies and maximum safe does was determined. The analgesic effect of the extracts showed significant dose dependent effect (100 mg/kg b.w and 200 mg/kg b.w) on both models of algesia i.e., Eddyâ€™s hotplate method and acetic acid induced writhing.
Conclusion: The comparative studies between the leaf and stem bark of Calophyllum inophyllum suggests that Calophyllum inophyllum leaf extract showed more activity compared to Calophyllum inophyllum stem bark extract.
Keywords: Calophyllum inophyllum, Calophyllum inophyllum leave extract, Calophyllum inophyllum stem bark extract, Analgesic effect, Eddyâ€™s hotplate method, Acetic acid induced writhing.
2. J. A.C. Dweck and T. Meadowsy Tamanu Calophyllum inophyllum the African Asian Polynesian and Pacic Panacea Int Science 24;2002:1-8.
3. Craig K, Prkachin K, Grunau R, Turk DC, Melzack R. The facial expression of pain. In and eds Hand book of pain assessment 1st edition Guilford New York;1992:257-76.
4. Julius D, Basbaum AI. Molecular mechanisms of nociception. Nature 2001;413(6852):203-10.
5. Rao MR, Rao YM, Rao AV, Prabhkar MC, Rao CS, Muraidhar N. Antinociceptive activity and antiinflammatory activity of a flavonoid Isolated from Caralluma attenuate. J Ethnopharmacol 1998;62:63-6.
6. Lavaud A, Soleti R, Hay AE, Richomme P, Guilet D, Andriantsitohaina R. Paradoxical effects of polyphenolic compounds from Clusiaceae on angiogenesis. PMID 22177987.
7. Syed B. Mirza Danish Silpa. S A Venkateshwara Reddy Antiinflammatory Activity of Ethanolic Extracts of Leaf and Stem Bark of Calophyllum inophyllum on Albino Wistar Rats Int J Pharm Sci Drug Res 2014;6(2):174-7.
8. Oecd. Guidelines Acute oral toxicity-Acute Oral Class methods. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas / Sociedade Brasileira de Biofisica [et al] 2001;423.
9. Eddy NB, Leimbach B. Synthetic analgesics II:Diathianyl and DithienyI butylamines. J Pharmacia 1953;107:385-93.
10. Witkin LB, Hebner CF, Gaddi F, Keefe E, Spitaletta P, Plumer AJ, et al. O' Pharmacology of 2-amino-indane hydrochloride (SU-8629):A potent non-narcotic analgesic. Exp Ther 1961;133:400-8.
11. Chakraborty A, Devi RKB, Rita S, Sharatchandra K, Singh TI. Preliminary studies on anti-inflammatory and analgesic activities of Spilanthes acmella in experimental animal models. International Journal Pharmacology 2004;36:148-50.
12. Shin Y.H. Kim S.C, Han J.W. Kim D.H Han SS. Shin D.H. and Nah, S.Y. Study on ginseng protopanaxadiol and saponins-induced antinociception. Korean Journal of Physiology and Pharmacology, 1997. 2:143-49.
13. Calixto JB. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas / Sociedade Brasileira de Biofisica [et al] 2000;33(2):179-89.
14. KÃ¼peli E, Yesilada E. Flavonoids with anti-inflammatory and antinociceptive activity from Cistus laurifolius L. leaves through bioassay-guided procedures. J Ethnopharmacol 2007;112(3):524-30.
15. Clavin M, Gorzalczany S, Macho A, MuÃ±oz E, Ferraro G, Acevedo C, et al. Anti-inflammatory activity of flavonoids from Eupatorium arnottianum. J Ethnopharmacol 2007;112(3):585-9.