THERAPEUTIC EFFICACY OF ISOLATED STIGMA-5,22 DIEN-3-O-B-D-GLUCOPYRANOSIDE AND ETHANOLIC ROOT EXTRACT OF OPERCULINA TURPETHUM AGAINST N- NITROSODIMETHYLAMINE INDUCED HEPATOPATHY IN THE LIVER OF MICE: ULTRASTRUCTURAL AND HISTOLOGICAL EVIDENCES
Objective: Liver is the most important organ in the pivotal role of regulating various physiological processes and several vital functions like metabolism, secretion and storage. Plants are reputed in the indigenous systems of medicine for the treatment of various diseases. Toxicant or drug induced liver injury can be prevented by treating with non toxic hepatoprotective herbs, which can possess membrane stabilizing, hepatoprotective and antioxidant activities. NDMA (N- Nitrosodimethylamine) belongs to nitrosamine compounds which are known hepatic carcinogens.
Methods: The aim of the present investigation was to analyse the effects of NDMA on the morphology of hepatic cells, to determine the reversible effect if any, after providing the treatment with the crude extract and isolated glycoside from the roots of Operculina turpethum. The treatment was given in different groups of Swiss Albino Mice.
Results: Scanning Electron Microscopy and Light microscopical examinations indicated that NDMA treated mice livers (n = 6) displayed severe vascular and endothelial damage compared to control livers (n = 6). Liver sections appeared with inflammatory cellular infiltration, vacuolated hepatocytes, dilated sinusoids, increased number of Kupffer cells, fibrosis, endothelial fenestrations, intercellular spaces and spaces of Disse, and were accompanied by dilatation of bile canaliculi.
Conclusion: These alterations were recovered with the treatment at the dose of 400 mg/kg of crude extract and 50 mg/kg of the isolated compound. Hence, it can be stated that this plant can show significant recovery in NDMA damaged livers.
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