ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NADIFLOXACIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Keywords:
Nadifloxacin, Bulk, Trifluoro acetic acid, Acetonitrile, HPLCAbstract
Objective: In the present work, a rapid, precise and sensitive HPLC Method with UV detection (237 nm) for analysis of Nadifloxacin in Bulk was developed.
Methods: Chromatography was performed with a mobile phase containing a mixture of 0.05 %v/v trifluoro acetic acid and acetonitrile (65:35 v/v) with flow rate 1.2 ml min-l. The proposed method was validated as per the standard guidelines.
Result: The retention time was found to be 12.3 min. In the range of 0.03-5 ppm, the linearity of Nadifloxacin shows a correlation co-efficient of 0.9997. Percentage recovery of the drug was found to be good (98-102%). Validation of the developed method was successful for precision, robustness, specificity and selectivity and ruggedness.
Conclusion: The developed HPLC method was found to be simple, sensitive, precise, accurate and reproducible and can be successfully used for the quantitative estimation of Nadifloxacin in bulk.
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References
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