ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NADIFLOXACIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

  • Ayyagari Rama Murthy Andhra University College of Engineering, Visakhapatnam 530003, India
  • Raghu Babu K. Andhra University College of Engineering, Visakhapatnam 530003, India
  • Ngaji Ambabhai Vekariya Aurobindo Pharma Ltd, Hyderabad, C-401, Gala Aria, South Bopal, Ahmedabad 380058

Abstract

Objective: In the present work, a rapid, precise and sensitive HPLC Method with UV detection (237 nm) for analysis of Nadifloxacin in Bulk was developed.

Methods: Chromatography was performed with a mobile phase containing a mixture of 0.05 %v/v trifluoro acetic acid and acetonitrile (65:35 v/v) with flow rate 1.2 ml min-l. The proposed method was validated as per the standard guidelines.

Result: The retention time was found to be 12.3 min. In the range of 0.03-5 ppm, the linearity of Nadifloxacin shows a correlation co-efficient of 0.9997. Percentage recovery of the drug was found to be good (98-102%). Validation of the developed method was successful for precision, robustness, specificity and selectivity and ruggedness.

Conclusion: The developed HPLC method was found to be simple, sensitive, precise, accurate and reproducible and can be successfully used for the quantitative estimation of Nadifloxacin in bulk.

 

Keywords: Nadifloxacin, Bulk, Trifluoro acetic acid, Acetonitrile, HPLC.

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References

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How to Cite
Murthy, A. R., R. B. K., and N. A. Vekariya. “ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NADIFLOXACIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 344-6, https://innovareacademics.in/journals/index.php/ijpps/article/view/1893.
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