SCAVENGING OF FREE RADICALS AND TOTAL PHENOLS OF METHANOL EXTRACT OF AZIMA TETRACANTHA LAM

  • Gayathri Gopalakrishnan Research Scholar, Dept. of Botany, University of Kerala, Kariavattom North Campus, Trivandrum 695 581
  • Bindu Nair Rajeshwari Nair Asst. Professor Dept. of Botany, University of Kerala, Kariavattom North Campus, Trivandrum 695 581
  • Babu V Professor Dept. of Botany, University College Trivandrum 695 034

Abstract

Objective: To report the antioxidant activity, phenolic acid profile of the Azima tetracantha methanolic extract and find the correlation between the total phenolic content and their relative antioxidant activity

Methods: The total antioxidant activity, hydroxyl radical and hydrogen peroxide scavenging of A. tetracantha methanol extract was evaluated by adopting various standard methods. Total phenolic content was estimated from dried methanolic extract and the phenolic acids in the extract were identified by HPLC.

Results: The methanolic extract of A. tetracantha showed free radical scavenging in a concentration dependant manner. The studies showed that the extract possess high free radical scavenging property, especially against hydroxyl radicals (67.61±0.42%). The correlation analysis results showed linear relation between phenolics content of methanolic extract and antioxidant assays (R2=0.97, 0.94, 0.99 and 0.98, significance at P<.0.001). The phenolic acid profile showed the presence of both benzoic acid and cinnamic acid derivatives, well known for their antioxidant potential.

Conclusion: The current observations suggested that the methanol extract of A. tetracantha exhibited multiple antioxidant activities, by scavenging free radicals. So, it can be concluded that the extract is a source of natural antioxidant, suggesting its traditional use as a rejuvenating agent.

 

Keywords: Azima tetracantha, Total antioxidant activity, Hydroxyl radical, Hydrogen peroxide, Total phenols, HPLC analysis.

Downloads

Download data is not yet available.

Author Biography

Gayathri Gopalakrishnan, Research Scholar, Dept. of Botany, University of Kerala, Kariavattom North Campus, Trivandrum 695 581

Department of Botany

Research Scholar

References

1. Harborne JB. Phytochemical Methods. Chapman and Hall: London; 1998.
2. Wong SP, Lai PL, Jen HWK. Antioxidant activities of aqueous extracts of selected plants. Food Chem 2005;99:775-83.
3. Miller NJ, Rice-Evans CA. Factors influencing the antioxidant activity determined by the ABTS+ radical cation assay. Free Radic Res 1997;26:195-9.
4. Halliwell B, Gutteridge JMC, Arouma OI. The deoxyribose method: a simple test tube assay for the determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 1987;165:215–9.
5. Ilavarasan R, Mallika M, Venkataraman S. Anti-inflammatory and antioxidant activities of Cassia fistula Linn. bark extracts. Afr J Trad CAM 2005;2(1):70–85.
6. Ruch RJ, Cheng SJ, Klaunig E. Prevention of cyto-toxicity and inhibition of intercellular communication by anti-oxidant catechins isolated from Chineese green tea. Carcinogenesis 1989;10:1003-8.
7. Mayr V, Treulter D, Santos-Buelga C, Bauee H, Feucht W. Developmental changes in the phenol concentration of golden delicious apple fruits and leaves. Phytochem 1995;38:1151.
8. Wong CC, Li HB, Cheng KW, Chen F. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem 2006:97:705–11.
9. Sanchez-Moreeno C. Methods used to evaluate the free radical activity in foods and biological systems. Food Sci Tech Int 2002;8:122.
10. Hazra Bibhabasu, Biswas Santanu, Mandal Nripendranath. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC CAM 2008;8:63.
11. Sahoo Suprava, Ghosh Goutam, Das Debajyoti, Nayak Sanghamitra, BL Burtt. Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra. Asian Pac J Trop Biomed 2013;3(11): 871-6.
12. Kumar S, Kumar D, Saroha K, Singh N, Vashishta B. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. Methanolic fruit extract. Acta Pharm 2008;58:215-20.
13. Thendral B, Hepsibha S, Sathiya C, Saravana Babu, Premalakshmi V, Sekar T. In vitro studies on antioxidant and free radical scavenging activities of Azima tetracantha. Lam. leaf extracts. IJST 2010;3(5): 571-7.
14. Muthuswamy P, Elakkiya S, Manjupriya K, Deepa K, Ramachandran S, Shanumgapandiyan P. Preliminary phytochemical and in vitro antioxidant perspectives of the leaf extracts of Azima tetracantha Lam (Family: Salvadoraceae). Int J Pharm Bio Sci 2012;3(1): 50-8.
15. Banerjee SK, Bonde CG. Total phenolic content and antioxidant activity of extracts of Bridelia retusa Spreng Bark: Impact of dielectric constant and geographical location. J Med Plants Res 2011;5(5):817–22.
16. Nadaroglu H, Demir Y, Demir N. Antioxidant and radical scavenging properties of Iris germanica. Pharm Chem J 2007;41(8):409–15.
17. Velioglu YS, Mazza L, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agric Food Chem 1998;46:4113-7.
18. Deighton N, Brennan R, Finn C, Davies HV. Antioxidant properties of domesticated and wild Rubus species. J Sci Food Agricul 2000;80:1307-13.
Statistics
225 Views | 4341 Downloads
How to Cite
Gopalakrishnan, G., B. N. R. Nair, and B. V. “SCAVENGING OF FREE RADICALS AND TOTAL PHENOLS OF METHANOL EXTRACT OF AZIMA TETRACANTHA LAM”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 347-51, https://innovareacademics.in/journals/index.php/ijpps/article/view/1894.
Section
Original Article(s)