ENHANCED APOPTOSIS IN MCF-7 HUMAN BREAST CANCER CELLS BY BIOGENIC GOLD NANOPARTICLES SYNTHESIZED FROM ARGEMONE MEXICANA LEAF EXTRACT
Keywords:A mexicana, Breast cancer, Gold nanoparticles, MCF-7 Cells, MTT assay
Objective: To assess the in vitro anticancer activity of A. mexicana mediated gold nanoparticles on MCF-7 breast cancer cells.
Methods: The present study reveals the synthesis and characterization of gold nanoparticles from aqueous extract of Argemone mexicana L. by biological method. UV-Vis spectroscopy, XRD, FT-IR, SEM and TEM analyses revealed that green synthesized gold nanoparticles were 26 Â± 5 nm in size and spherical in shape. MCF- 7 Breast cancer cells were treated with different concentrations of green synthesised gold nanoparticles at different time intervals. Cytotoxicity activity and the mechanism of apoptosis were determined by morphological and chromatin cleavage assays.
Results: Green synthesised gold nanoparticles showed dose-dependent cytotoxic and apoptotic effect in MCF-7 breast cancer cells at concentration of 100 Î¼g/mL.
Conclusion: The present investigation showed that Argemone mediated gold nanoparticles may be an alternative chemotherapeutic agent for treatment of breast cancer.
Wu CC, Chen DH. Facile green synthesis of gold nanoparticles with gum arabic as a stabilizing agent and reducing agent. J Gold Bull 2010;43:234â€“9.
Daniel MC, Astruc D. Gold nanoparticles:assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. J Chem Rev 2004;104:293-346.
Kreibig U, Vollmer M. Optical properties of metal clusters. Springer-Verlag, Berlin;1995.
Tiwari PM, Vig K, Dennis VA, Singh SR. Functionalized gold nanoparticles and their biomedical applications. J Nanomaterials 2011;1:31-63.
Gardea-Torresdey JL, Parsons JG, Gomez E, Peralta-Videa J, Troiani HE, Santiago P, Jose-Yacaman M. Formation and growth of Au nanoparticles inside live Alfalfa Plants. J Nano Letters 2002;2:397-401.
Ankamwar B, Damle C, Ahmad A, Sastry M. Biosynthesis of gold and silver nanoparticles using Emblics Officinalis fruit extract and their phase transfer and transmetallation in an organic solution. J Nanosci Nanotechnol 2005;5:1665-71.
Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, et al. Biosynthesis of silver and gold nanoparticles by novel sundried Cinna-mommum camphora leaf. J Nanotechnology 2007;18:105104-15.
Parashar V, Parashar R, Sharma B, Avinash Pandey C. Parthenium leaf extract mediated synthesis of silver nanoparticles:a novel approach towards weed utilization. Dig J Nanomater Bios 2000;4:45-50.
Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants, Council of Scientific and Industrial Research:New Delhi:1986.
Noruzi M, Zare D, Khoshnevisan K, Davoodi D. Rapid green synthesis of gold nanoparticles using Rosa hybrida petal extract at room temperature. J Spectrochim Acta Part A 2011;79:1461â€“65.
Shankar SS, Rai A, Ankamwar B, Ahmad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 2004;15:496-502.
Selim ME, Hendi AA. Gold nanoparticles induce apoptosis in MCF-7 human breast cancer cells. Asian Pacific J Cancer Prev 2012;13:1617-20.
Prathyumnan S, Sellappa S, Subhadra Keyan K, Joseph S. Enhanced calreticulin expression triggers apoptosis in the MCF-7 cell line. Asian Pacific J Cancer Prev 2010;11:1133-36.
Hongo T, Mizun Y, Haraguch S, Yoshida TO. A new anticancer drug sensitivity test using the microplate culture and surviving tumor cell staining method. J Gan To Kagaku Ryoho 1986;13:247-54.
Riedl SJ, Shi Y. Molecular mechanisms of caspase regulation during apoptosis. J Nat Rev Mol Cell Biol 2004;5:897-907.
Yadav S, Seer H, Murthy RR. Cytotoxic, apoptotic and anti angiogenic efficacy of silver nanoparticles, synthesized using tannic acid as reducing agent under controlled temperature. Int J Pharm Bio Sci 2012;1:160-77.