• Syed Ali Fathima M. Centre for Plant Biotechnology, Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tamil Nadu, India - 627 002
  • Johnson M Centre for Plant Biotechnology, Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tamil Nadu, India - 627 002


Objective: The present study was aimed to reveal the spectroscopic profile (UV-Vis and FT-IR) of Pouzolzia wightii Benn.

Methods: To detect the UV-Vis spectroscopic profile of P. wightii crude extracts were examined under UV-Vis Shimadzu spectrophotometer with the wavelength ranged from 100 to 1100 nm. About 1 mg of different extracts of petroleum ether, chloroform, ethyl acetate and acetone, ethanolic extracts of P. wightii were separately made into thin discs with 10-100 mg of potassium bromide using a mould and pressed under anhydrous conditions. The pellets were measured in an automatic recording FT-IR Spectrophotometer (Shimadzu 8400S) in the range of 400 to 4000 cm-1

Results: In UV-Vis analysis, P. wightii petroleum ether extracts showed more number of peaks in roots (15) than other studied parts. Chloroform and ethyl acetate extracts of P. wightii leaves observed 9 peaks and acetone extracts of P. wightii stem showed 10 peaks. Medicinal property of plant extracts are confirmed by the presence of secondary metabolites. FT-IR analysis of ethyl acetate extracts of P. wightii leaves, stem and root observed the highest number of (16, 12 and 16) functional compounds.

Conclusion: These UV-Vis and FT-IR spectroscopic results may be used as a pharmacognostic marker in the pharmaceutical industries and can be used as a chemometric tool to distinguish the studied P. wightii leaves, stem and root. The present study used to find out the bioactive compounds which may be subjected to subsequent target isolation. Further research will be needed for the structural characterization of the isolated compound by the use of different analytical methods such as NMR and mass spectrophotometer.

Keywords: Pouzolzia wightii, FT-IR-Fourier Transform-Infra Red, UV-Vis-Ultra Violet–Visible, Pharmacognosy


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1. Ghani A. Medicinal plants of Bangladesh with chemical constituents and uses. 2nd edition. Bangladesh: Asiatic Society of Bangladesh, Dhaka; 2003.
2. Ashis G. Herbal folk remedies of Bankura and Medinipur districts, West Bengal. Indian J Traditional Knowledge 2003;2:393-6.
3. Sakanaka S, Tachibana Y, Okada Y. Preparation and antioxidant properties of extracts of Japanese permimmon leaf tea (kakinocho-cha). Food Chem 2005;89:569-75.
4. Argal A, Pathak AK. CNS activity of Calotropis gigantean roots. J Ethnopharmacol 2006;106:142-5.
5. Parekh J, Chanda V. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turkish J Biol 2007;31:53-8.
6. Ibrahim M, Hameed AJ, Jalbout A. Molecular spectroscopic study of river nile sediment in the greater cairo region. Appl Spectroscopy 2008;62:306-11.
7. Yang J, Yen HCE. Early salt stress effects on the changes in chemical composition in leaves of ice plant and Arabidopsis. A fourier transform infrared spectroscopy study. Plant Physiol 2002;130:1032-42.
8. Surewicz WK, Mantsch HH, Chapman D. Determination of protein secondary structure by fourier transform infrared spectroscopy: a critical assessment. Biochemistry 1993;32: 389-93.
9. Mistry BD. A Handbook of spectroscopic data. Jaipur, India: Oxford Book Company; 2009.
10. Kumar JK, Devi Prasad AG. Identification and comparison of biomolecules in medicinal plants of Tephrosia tinctoria and Atylosia albicans by using FT-IR. Romanian J Biophys 2011;21:63-71.
11. Thenmozhi M, Bhavya PK, Rajeswari S. Compound isolation using HPLC and FT-IR in Eclipta alba and Emilia sanchifolia. Int J Eng Technol 2011;3:292-8.
12. Nair R, Chanda S. Activity of some medicinal plants against the certain pathogenic bacterial strain. Indian J Pharmacol 2006;38:142-4.
13. Yamuna M, Gnanaraj WE, Johnson M. FT-IR spectroscopic studies on Aerva lanata (L.) Juss. Ex. Schult. Asian J Pharm Clin Res 2012;5:82-6.
14. Rani N, Sharma S, Mukta Sharma. Phytochemical Analysis of Meizotropis pellita by FT-IR and UV-Vis spectrophotometer. Indian J Sci Technol 2016;9:1-4.
15. Prasanna G, Anuradha R. Ultraviolet-visible and fourier transform-infrared spectroscopic studies on Drynaria quercifolia L. Rhizome Asian J Pharm Clin Res 2016;9:85-8.
16. Sahaya Sathish S, Janakiraman N, Johnson M. Phytochemical analysis of Vitex altissima L. using UV-Vis, FT-IR and GC-MS. Int J Pharm Sci Drug Res 2012;4:56-62.
17. Sahu R, Saxena J. Ultraviolet-visible and fourier transform infrared spectroscopic studies on non-conventional species of Curcuma. Indian J Adv Chem Sci 2014;2:300-2.
18. Mamta S, Jyoti S. Evaluation of phytoconstituents of Acorus calamus by FT-IR and UV-Vis spectroscopic analysis. Int J Biol Pharm Res 2012;3:498-501.
19. Neha S, Jyoti S. Phytochemical analysis of Bougainvillea glabra choisy by FT-IR and UV-Vis spectroscopic analysis. Int J Pharm Sci Rev Res 2013;2:196-8.
20. Renuka B, Sanjeev B, Ranganathan D. Evaluation of phytoconstituents of Caralluma nilagiriana by FT-IR and UV-Vis spectroscopic analysis. J Pharmacognosy Phytochem 2016;5:105-8.
21. Bunghez F, Socaciu C, Zăgrean F, Pop MR, Ranga F, Romanciuc F. Characterisation of an aromatic plant-based formula using UV-Vis spectroscopy, LC-ESI(+)QTOF-MS and HPLC-DAD analysis bulletin UASVM. Food Sci Technol 2013;70:16-24.
22. Anand T, Godula Krishnan. Phytochemical analysis of Hybanthus enneaspermus using UV, FT-IR and GC-MS. IOSR J Pharm 2012;2:520-4.
23. Bobby MDN, Wesely EG, Johnson M. FT-IR studies on the leaves of Albizia lebbeck benth. Int J Pharm Pharm Sci 2012;4:293-6.
24. Ragavendran P, Sophia D, Arul Raj C, Gopalakrishnan VK. Functional group analysis of a various extract of Aerva lanata (L.) by FT-IR spectrum. Pharmacologyonline 2011;1:358-64.
25. Helm D, Labischinski H, Schallehn G, Naumann D. Classification and identification of bacteria by fourier transform infrared spectroscopy. J Gen Microbiol 1991;137:69-79.
26. Kim SW, Ban SH, Chung H, Cho S, Chung HJ, Chol PS, et al. Taxonomic discrimination of flowering plants by multivariate analysis of fourier transform infrared spectroscopy data. Plant Cell Reports 2004;23:246-50.
27. Janakiraman N, Sahaya Sathish S, Johnson M. UV-Vis and FTIR spectroscopic studies on Peristrophe bicalyculata (Retz,) Nees. Asian J Pharm Clin Res 2011;4:125-9.
28. Janakiraman N, Johnson M. Functional groups of tree ferns (Cyathea) Using FT-IR: chemotaxonomic implications. Rom J Biophys 2015;25:131–41.
29. Johnson M, Petchiammal E. Phytochemical characterization of Sargassum swartzii (Turner) C. Agardh using thin-layer chromatography, Fourier transform infrared and high-performance liquid chromatography. J Med Herbs Ethnomed 2015;1:120-4.
30. Sim CO, Hamdan MR, Ismail Z, Ahmad MN. Assessment of herbal medicines by chemometrics–assisted Interpretation of FT-IR spectra. J Anal Chim Acta 2004;570:1-14.
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How to Cite
M., S. A. F., and J. M. “SPECTROSCOPIC STUDIES ON POUZOLZIA WIGHTII BENN”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 3, Mar. 2018, pp. 124-32, doi:10.22159/ijpps.2018v10i3.19336.
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