SPECTROSCOPIC STUDIES ON POUZOLZIA WIGHTII BENN
Objective: The present study was aimed to reveal the spectroscopic profile (UV-Vis and FT-IR) of Pouzolzia wightii Benn.
Methods: To detect the UV-Vis spectroscopic profile of P. wightii crude extracts were examined under UV-Vis Shimadzu spectrophotometer with the wavelength ranged from 100 to 1100 nm. About 1 mg of different extracts of petroleum ether, chloroform, ethyl acetate and acetone, ethanolic extracts of P. wightii were separately made into thin discs with 10-100 mg of potassium bromide using a mould and pressed under anhydrous conditions. The pellets were measured in an automatic recording FT-IR Spectrophotometer (Shimadzu 8400S) in the range of 400 to 4000 cm-1
Results: In UV-Vis analysis, P. wightii petroleum ether extracts showed more number of peaks in roots (15) than other studied parts. Chloroform and ethyl acetate extracts of P. wightii leaves observed 9 peaks and acetone extracts of P. wightii stem showed 10 peaks. Medicinal property of plant extracts are confirmed by the presence of secondary metabolites. FT-IR analysis of ethyl acetate extracts of P. wightii leaves, stem and root observed the highest number of (16, 12 and 16) functional compounds.
Conclusion: These UV-Vis and FT-IR spectroscopic results may be used as a pharmacognostic marker in the pharmaceutical industries and can be used as a chemometric tool to distinguish the studied P. wightii leaves, stem and root. The present study used to find out the bioactive compounds which may be subjected to subsequent target isolation. Further research will be needed for the structural characterization of the isolated compound by the use of different analytical methods such as NMR and mass spectrophotometer.
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