A VALIDATED ISOCRATIC RP-HPLC METHOD FOR CONCURRENT ESTIMATION OF GYMNEMAGENIN, GALLIC ACID AND 18Β-GLYCYRRHETINIC ACID IN POLYHERBAL FORMULATION


Sachin Eknath Potawale, Pravin Devidas Pawar, Satish Yashwant Gabhe, Kakasaheb Ramoo Mahadik

Abstract


Objective: To develop and validate a simple, precise, selective, and accurate reversed phase high performance liquid chromatography method for concurrent analysis of gymnemagenin, gallic acid and 18β-glycyrrhetinic acid in polyherbal formulation.

Methods: The chromatographic separation was achieved on a Thermo Synchronis C18, 5 μm, 250 × 4.6 mm i. d. analytical column. The mobile phase comprised of methanol: water (88: 12, v/v), pH 3.1 adjusted with orthophosphoric acid. The flow rate was kept at 0.8 mL min-1. Quantitation was achieved with UV detection at 218 nm, based on peak area.

Results: The retention time for gallic acid, gymnemagenin, and 18β-glycyrrhetinic acid was found to be 3.08, 4.15, and 10.30 min, respectively. Validation of the RP-HPLC method was performed as per International Conference on Harmonization (ICH) Q2 (R1) guideline. The proposed method showed good linearity in the range of 100-1000 μg mL-1 for gymnemagenin, 2.5-50 μg mL-1 for gallic acid and 50-500 μg mL-1 for 18β-glycyrrhetinic acid. The % content of gymnemagenin, gallic acid and 18β-glycyrrhetinic acid in the marketed formulation was found to be 0.1320, 0.2129 and 0.2799 %, respectively.

Conclusion: The proposed method can be useful in the quality control of gymnemagenin, gallic acid and 18β-glycyrrhetinic acid in polyherbal formulation.


Keywords


Gymnemagenin, Gallic acid, 18β-glycyrrhetinic acid, Isocratic HPLC, ICH.

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About this article

Title

A VALIDATED ISOCRATIC RP-HPLC METHOD FOR CONCURRENT ESTIMATION OF GYMNEMAGENIN, GALLIC ACID AND 18Β-GLYCYRRHETINIC ACID IN POLYHERBAL FORMULATION

Keywords

Gymnemagenin, Gallic acid, 18β-glycyrrhetinic acid, Isocratic HPLC, ICH.

Date

01-09-2014

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 6, Issue 8, 2014 Page: 488-492

Online ISSN

0975-1491

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556 Views | 298 Downloads

Authors & Affiliations

Sachin Eknath Potawale
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University (BVDU), Pune 411038, India.
India

Pravin Devidas Pawar
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University (BVDU), Pune 411038, India.
India

Satish Yashwant Gabhe
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University (BVDU), Pune 411038, India.
India

Kakasaheb Ramoo Mahadik
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University (BVDU), Pune 411038, India.
India


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