EVALUATION OF THE WOUND HEALING ACTIVITY OF CAESALPINIA BONDUCELLA AND CYCLEA PELTATA EXTRACTS IN EXPERIMENTALLY INDUCED DIABETIC RATS


Jagadeep Chandra S., Mahadeva Murthy S., Ranjana Ramesh

Abstract


Objective: The present study was conducted to verify the effect of Caesalpinia bonducella and Cyclea peltata extracts on experimentally induced excision wounds in diabetic rats.

Methods: Methanolic and ethyl acetate extract of test sample in PEG base was applied and observed for wound healing effect for a period of 15 d.

Results: The data demonstrated statistically significant wound contraction up to 98% in the treated sample as compared to 90% in diabetic control group. The results correlated (r ³ 0.95) with fasting blood glucose level, indicating the deleterious effect of hyperglycemia in wound healing. Histopathological studies indicated moderate granulation with marked epithelial enclose and moderate hyperplasia in groups treated with high dose (100 mg/kg BW) of methanolic extract of aerial part of Cyclea peltata and root of Caesalpinia bonducella.

Conclusion: From the study, it can be hypothesized that tested plant extracts favours wound healing in diabetic animals and paves the way for the more detailed study of the phytochemical constituent for therapeutic applications. The present investigation adds to the existing knowledge in the field of therapeutic medicine and may even become the base for the development of herbal based gel formulations or ointments for treating wounds in diabetic patients and thereby continuous usage of synthetic drugs, its associated side effects could be avoided.


Keywords


Wound healing, Diabetic rats, Blood glucose, Histopathology and Excision wounds

| PDF | HTML |

References


Nagori BP, Solanki R. Role of medicinal plants in wound healing. Res J Med Plant 2011;5:392–405.

Pierce GF, Mustoe TA. Pharmacologic enhancement of wound healing. Annu Rev Med 1995;46:467-81.

Martin P. Wound healing aiming for perfect skin regeneration. Science 1997;276:75–81.

Sandhya S, SaiKumar P, Vinod KR, Banji D, Kumar K. Plants as potent antidiabetic and wound healing agents: a review. Hygeia J Drugs Med 2011;3:11-9.

Powers CA. Diabetes mellitus infections: Harrison’s principles of Internal Medicine. 16th edition. McGraw Hill publishers (p) Ltd; 2004. p. 2168-9.

Brem H, Tomic-Canic M. Cellular and molecular basis of wound healing in diabetes. J Clin Investigation 2007;117:1219-22.

Grey JE, Harding KG. ABC of wound healing. Wiley Blackwell; 2006. p. 42.

Falanga V. The chronic wound: impaired wound healing and solutions in the context of wound bed preparation. Blood Cells Mol Diseases 2004;32:88.

Osariemen IJ, Olowu SS, Adevbo E, Omon EE, Victoria O, Imuetinyan EJ, et al. Aerobic bacteria associated with diabetic wounds in patients attending the clinic in a rural community in Nigeria. Global Res J Microbiol 2013;3:8-12.

Karodi R, Jadhav M, Rub R, Bafna A. Evaluation of the wound healing activity of a crude extract of Rubia cordifolia L. (Indian madder) in mice. Int J Appl Res Natl Prod 2009;2:12-8.

Mukherjee PK, Mukherjee K, Pal M, Saha BP. Wound healing potential of Nelumbo nucifera (Nymphaceae) rhizome extract. Phytomedicine 2000;7:66-73.

Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. Ethnopharmacological approaches to wound healing-exploring medicinal plants of India. J Ethnopharmacol 2007;114:103-13.

Reddy JS, Rajeswara Rao P, Reddy MS. Wound healing effects of Heliotropium indicum, Plumbago zeylanicum and Acalypha indica in rats. J Ethnopharmacol 2002;79:249–51.

Pulok KM, Verpoorte R, Suresh B. Evaluation of in vivo wound healing activity of Hypericum patulum (Family: Hypericaceae) leaf extract on different wound model in rats. J Ethnopharmacol 2000;70:315-21.

Handa SS, Kaul MK. Supplement to cultivation and utilization of medicinal plants. RRL Jammu–Tawi; 1996. p. 727-37.

Kirtikar, Basu. Indian Medicinal Plants. 2nd Editor. B.S.M.P. Singh and Periodical Experts, New Delhi; 1975;2:842.

Kapoor LD. Handbook of Ayurvedic Medicinal Plants. 1st Ed. CRC Press: Washington; 2005. p. 87-8.

The Wealth of India. Raw material, Ca-Ci, Revised Edt, Publication and Information Directorate, CS I R, New Delhi; 1992;3:6-8.

Shrikant I. Further flowers of Sahyadri “Field guide to additional 1200 flowers of Northwestern ghats of India. Corolla Publications-Pune, India; 2007. p. 345.

Deshpande DJ. A handbook of herbal remedies. Published by Agrobios (India)-Jodhpur; 2008. p. 232-3.

Yoganarasimhan SN. Medicinal plants of India. Vol. I. Interline publishing private limited-Banglore, Karnataka; 1996. p. 82.

Nadkarni AK, KM Nadkarni’s. Indian Materia Medica. Popular prakashan private limited, Bombay; 2002. p. 226-9.

Elizabeth M, Williamson. Textbook of Major herbs of Ayurveda. Churchill (I); 2002. p. 7-11.

Nair CKN, Mohanan N. Medicinal plants of India (With special reference to Ayurveda). Nag Publishers, Delhi; 1998. p. 80.

Ambasta SPK, Kashypa, Ramchandran K, Ramesh Chand R. Ed. Useful plants of India. Publications Div, CSIR, Directorate, New Delhi, India; 1986.

Subramanian R, Krishnasamy G, Arunadevaraj, Sethuraman P, Jayakumararaj R. Wound healing ethnopharmacological potentials of selected medicinal plants used by malayali tribes. Int Res J Pharmacol 2011;2:132-7.

Vaidyaratnam PSV. Indian medicinal plants (a compendium of 500 species). Orient Longman Limited 1997;2:277.

Suman S, Nishteswar K. Review on Cissampelospareira and Cycleapeltata (Pathadwaya) phytopharmacological perspectives. Int J Ayurvedic Med 2013a;4:282-9.

Christina AJM, PackiaLakshmi M, Nagarajan M, Kurian S. Modulatory effect of Cycleapeltata Lam. on stone formation induced by ethylene glycol treatment in rats. Methods Find Exp Clin Pharmacol 2002;24:77-9.

Suman S, Nisteshwar K. Review of Cissampelo spareira and Cycleapeltata (Pathadwaya) ethnomedicinal perspectives. Global J Pharm Res 2013b;2:1656-62.

Purohit SS, Narayan DP, Arun KS, Kumar T. A Handbook of Medicinal Plants. Agrobios, India; 2003. p. 184.

Vijayakirubha TS, Senthamarai R, Mariya P, Praveen M. Pharmacognostical and phytochemical standards of Cycleapeltata. (Lam) Hook. f and Thomson leaves. J Chem Pharm Res 2012;4:1465-9.

Vaidyaratnam PSV. Indian medicinal plants (a compendium of 500 species). Published by Orient Longman Limited; 1997. p. 277.

Oommen S, Ved DK, Krishnan R. Tropical Indian medicinal plants propagation methods. Published by Foundation for the revitalisation of local health traditions, Banglore; 2000. p. 125-6.

Raj DAR, Jeeva S, Prakash JW, Antonisamy JM, Irudayaraj V. Antibacterial activity of selected ethnomedicinal plants from South India. Asian Pacific J Trop Med 2011;4:375-8.

Kingston C, Nisha BS, Kiruba S, Jeeva S. Ethnomedicinal plants used by the indigenous community in the traditional healthcare system. Ethnobot Leaflets 2007;11:32-7.

Bhandary MJ, Chandrashekar KR, Kaveriappa KM. Medical ethnobotany of the Siddis of Uttara Kannada district, Karnataka, India. J Ethnopharmacol 1995;47:149-58.

Bhandary MJ, Chandrashekar KR. Herbal therapy for herpes in the ethnomedicine of coastal Karnataka. Indian J Traditional Knowledge 2011;10:528-32.

Santhkumari P, Prakasam A, Pugalendi KV. Modulation of oxidative stress parameters by treatment with Piper beetle leaf in streptozotocin-induced diabetic rats. Int J Pharmacol 2003;35:373-8.

Kapoor R, Kakkar P. Naringenin accords hepatoprotection from streptozotocin-induced diabetes in vivo by modulating mitochondrial dysfunction and apoptotic signalling cascade. Toxicol Reports 2014;1:569-81.

Suguna L, Shivakumar P, Chandrakasan G. Effect of Centella asiatica extract on dermal wound healing in rats. Indian J Exp Biol 1996;34:1208-11.

Jagdish VK, Rana AC, Anirban RC. Prohealing effects of Cinnamomum zeylanicum bark. Phytother Res 2003;17:970-2.

Boehmerle W, Huehnchen P, Peruzzaro S, Balkaya M, Endres M. Electrophysiological, behavioural and histological characterization of paclitaxel, cisplatin, vincristine and bortezomib-induced neuropathy in C57Bl/6 mice. Sci Rep 2014;4. Doi:10.1038/srep06370

Agarwal PKA, Singh K, Gaurav S, Goel H, Khanna D, Goel RK. Evaluation of wound healing activity of extracts of Plantain banana (Musa sapientum var. Paradisiaca) in rats. Indian J Exp Biol 2009;47:32–40.

Zaouani M, Bitam A, Baz A, Nenali Y, Ben-Mahdi MH. In vivo evaluation of wound healing and anti-inflammatory activity of methanolic extract of roots of Centaurea africana (L.) in a topical formulation. Asian J Pharm Clin Res 2017;10:341-6.

Sunilkumar, Parameshwaraiah S, Shivakumar HG. Evaluation of topical formulations of aqueous extract of Centella asiatica on open wounds in rats. Indian J Exp Biol 1998;36:569-72.

Lan CCE, Wu CS, Huang SM, Wu IH, Chen GS. High glucose environment enhanced oxidative stress and increased interleukin-8 secretion from keratinocytes. Diabetes 2013;62:2530-8.

Yazdanpanah L, Nasiri M, Adarvishi S. Literature review on the management of diabetic foot ulcer. World J Diabetes 2015;6:37-53.

Gautam MK, Goel RK. Wound healing effect of Ocimum sanctum leaves extract in diabetic rats. World Academ Sci Eng Technol 2013;81:144-7.

Khatri SK, Rathman and M, Nikhila R. Formulation and evaluation of wound healing activity of linezolid topical preparation on diabetic rats. Int J Appl Pharm 2016;8:30-6.

Singhal AK, Gupta H, Bhati VS. Wound healing activity of Argyreia nervosa leaves extract. Int J Appl Basic Med Res 2011;1:36-9.

Vinothapooshan G, Sundar K. Wound healing effect of various extracts of Adhatoda vasica. Int J Pharma Biol Sci 2010; 1:530–6.

Kodati DR, Goud KP, Burra A, Galipelly SK. Evaluation of wound healing activity of methanolic root extract of Plumbago zeylanica L. in wistar albino rats. Pelagia Res Library Pharm Sinica 2011;2:239-48.

Murthy S, Gautam MK, Goel S, Purohit V, Sharma H, Goel RK. Evaluation of in vivo wound healing activity of Bacopa monniera on different wound model in rats. Biomed Res Int 2013;1-9. http://dx.doi.org/10.1155/2013/972028

Moyer KE, Saggers GC, Allison GM, Mackay DR, Ehrlich HP. Effects of interleukin-8 on granulation tissue maturation. J Cell Physiol 2002;193:173–9.

Farahpour MR, Habibi M. Evaluation of the wound healing activity of an ethanolic extract of Ceylon cinnamomum in mice. J Vet Med 2012;57:53-7.

Mageswari S, Karpagam S, Aadinaath Reddy G. Evaluation of wound healing activity of the plant Carmona retusa (vahl) masam, in mice. Int J Integr Sci Innovation Technol 2015;4:1-4.

Singh R, Koppikar SJ, Paul P, Gilda S, Paradkar AR, Kaul-Ghanekar R. Comparative analysis of the cytotoxic effect of aqueous cinnamon extract from Cinnamomum zeylanicum bark with commercial cinnamaldehyde on various cell lines. Pharm Biol 2009;47:1174–6.

Singh S, Sharma N. Evaluation of wound healing activity of Acacia auriculiformis A. Cunn. Stem bark. Asian J Pharm Clin Res 2014;17:204-7.




About this article

Title

EVALUATION OF THE WOUND HEALING ACTIVITY OF CAESALPINIA BONDUCELLA AND CYCLEA PELTATA EXTRACTS IN EXPERIMENTALLY INDUCED DIABETIC RATS

Keywords

Wound healing, Diabetic rats, Blood glucose, Histopathology and Excision wounds

DOI

10.22159/ijpps.2017v9i10.19788

Date

02-10-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol. 9, Issue 10, 2017 Page: 211-217

Online ISSN

0975-1491

Statistics

0 Views | 0 Downloads

Authors & Affiliations

Jagadeep Chandra S.
1Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysuru 570006, India
India

Mahadeva Murthy S.
Department of Microbiology, Yuvaraja’s College, University of Mysore, Mysuru 570005, India
India

Ranjana Ramesh
Skanda LifeScience Ltd., Bangalore 560091, India
India


Article Tools



Refbacks

  • There are currently no refbacks.