THE POTENTIAL OF CANARIUM ODONTOPHYLLUM MIQ. (DABAI) AS ANTI-METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS AGENT
Keywords:Canarium odontophyllum, Antimicrobial, Disc diffusion, MRSA, MIC, MBC
Objective: The present study evaluates the antimicrobial potential of C. Odontophyllum leaves against Methicillin-resistant Staphylococcus aureus, Candida albicans, Candida glabrata, Candida krusei, Candida tropicalis, Â Aspergillus fumigatus, Aspergillus niger and Aspergillus flavus.
Materials: The extracts from C. odontophyllum leaf were prepared using acetone, methanol and distilled waterprior to screening at concentrations from12.5 mg/ml to 100 mg/ml against the test microorganisms using disc diffusion method. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts against susceptible organisms were determined using microbroth dilution method and streak-plate technique, respectively.
Results: Water produced the highest yield of extract (5.03%) followed by methanol (2.65%) and acetone (1.79%). Out of all the microbes tested, only MRSA was found to be susceptible towards acetone and methanol extracts of C. odontophyllum leaves which showed concentration-dependent growth inhibitory effect against MRSA. Despite the highest extractive potential of water, no antimicrobial activity was observed by the aqueous extract from the screening assay. The MIC values for methanol and acetone extracts were respectively,6.25 mg/ml and 3.125 mg/ml. The MBC value of methanol extract was twice its MIC value which was 12.5 mg/ml whereas the MIC and MBC values of acetone extract against MRSA were the same (3.125 mg/ml).
Conclusion: C. odontophyllum leaves have the potential to be developed as an alternative phytotherapeutic agent against MRSA infection.
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