ANTIDIABETIC EFFECT OF EXTRACTS OF BLUMEA LACERA DC. IN STREPTOZOTOCIN INDUCED HYPERGLYCEMIC RATS
Keywords:Blumea lacera, Metformin, Blood glucose level, Bio-chemical level, Insulin, Glycated hemoglobin
Objective: Present study of this research was undertaken to evaluate the antidiabetic activities of extracts of aerial parts of Blumea lacera DC. (Asteraceae) in streptozotocin (STZ) induced hyperglycemic rat.
Methods: The methanol extracts (MEBL) and aqueous extract (AEBL) of B. lacera DC. were investigated in streptozotocin (STZ) induced hyperglycemic rats at a dose level of 200 and 400 mg/kg body weight, in oral glucose tolerance test (OGTT), acute and subacute antidiabetic (30 d) models keeping a parallel group of metformin (250 mg/kg body weight) as standard drug. The serum biochemical parameters, histopathology of liver and pancreaswere examined and analyzed statistically.
Results: Treatment with methanol extract of B. lacera (MEBL) at a dose of 200 mg/kg and 400 mg/kg body weight, significantly decrease (p<0.05) blood glucose level from 289.83Â±9.83 and 289.83Â±2.71 to 201.83Â±8.87 and 105.00Â±2.05 respectively with corresponding percentage fall of blood glucose to 30.40Â±1.79 and 63.78Â±0.59. It also improved the glycated haemoglobin (HbA1c) near to normal value, restored the lipid and bio-chemical level and rejuvenate beta cells of pancreas, thereby improve insulin secretion.
Conclusion: The result of the present study concluded that extracts of B. lacera DC possess marked antidiabetic activity. However, methanol extract of B. lacera (MEBL) at the dose of 400 mg/kg showed significant dose dependent antidiabetic effects without any destruction and restores the structure of liver and pancreas of hyperglycemic rats.
K. Arunachalam and T. Parimelazhagan, Antidiabetic activity of Ficus amplissima Smith. bark extract in streptozotocin induced diabetic rats,â€ J. Ethnopharmacol., vol. 147, no. 2, pp. 302â€“310, 2013.
C. . Khare, Indian Medicinal Plants - An illustrated dictionary. New Delhi: Springer, 2007.
M. Salahuddin et al., Medicinal plants used by a Traditional Ayurvedic Practitioner at Asadnagar Village in Narsingdi District , Bangladesh,â€ J. Pharmacogn. Phytochem., vol. 4, no. 2, pp. 269â€“275, 2015.
U. P. Singh, A. K. Singh, and R. P. Sarathy, Effect of methanolic extracts of Blumea Eriantha DC. leaves on protein metabolism and marker enzymes in streptozotocin-induced hyperglycemic animals,â€ Int. J. Pharm. Pharm. Sci., vol. 4, pp. 1â€“4, 2012.
M. N. Hasan, M. H. Rahman, R. Guo, and A. Hirashima, Hypoglycemic activity of methanolic leaf extract of Blumea lacera in Swiss-albino mice,â€ Asian Pacific J. Trop. Dis., vol. 5, no. 1, pp. 195â€“198, 2015.
M. Ibrahim, A. Khair, M. Ibrahim, Q. Ahsan, and Z. Homa, Pharmacological Activities of Blumea lacera ( Burm . f ) DC : A Medicinal Plant of Bangladesh Plant of Bangladesh,â€ Br. J. Pharm. Res., vol. 4, no. January 2016, pp. 1677â€“1687, 2014.
S. Ram and V. S. Sinha, Qualitative phytochemical analysis of some plants use to cure Malaria in Kolhan region , of Jharkhand, India,â€ J. Med. Plants Stud., vol. 3, no. 6, pp. 60â€“62, 2015.
OECD/OCDE, Acute oral toxicity - Acute toxic class method,â€ 2001.
M. C. Garcia-Galicia, E. BurguenoTapia, A. Romero-Rojas, J. C. GarcÃa-ZebadÃºa, C. Ordaz-Pichardo, and J. Cornejo-Garrido, Anti-hyperglycemic effect, inhibition of inflammatory cytokines expression, and histopathology profile in streptozotocin-induced diabetic rats treated with Arracacia tolucensis aerial-parts extracts,â€ J. Ethnopharmacol., vol. 152, no. 1, pp. 91â€“98, 2014.
D. Rath, D. M. Kar, S. K. Panigrahi, and L. Maharana, Antidiabetic effects of Cuscuta reflexa Roxb. in streptozotocin induced diabetic rats,â€ J. Ethnopharmacol., vol. 192, pp. 442â€“449, 2016.
M. Aslan, N. Orhan, D. D. Orhan, and F. Ergun, Hypoglycemic activity and antioxidant potential of some medicinal plants traditionally used in Turkey for diabetes,â€ J. Ethnopharmacol., vol. 128, no. 2, pp. 384â€“389, 2010.
M.-C. Tchamadeu et al., Hypoglycaemic effects of Mammea africana ( Guttiferae ) in diabetic rats,â€ J. Ethnopharmacol., vol. 127, pp. 368â€“372, 2010.
CPCSEA, CPCSEA guidelines for laboratory animal facility,â€ Indian J Pharmacol, vol. 35, pp. 257â€“274, 2003.