EVALUATING THE PROTECTIVE EFFICACY OF COMBINATION AND WITHANIA SOMNIFERA AND VITAMIN E AGAINST CADMIUM INDUCED OXIDATIVE STRESS MEDIATED HEPATIC HISTOPATHOLOGY AND GENOTOXICITY IN MURINE MODEL


Seema Rani, Vijay Lakshmi Sharma, Mani Chopra

Abstract


Objective: Cadmium (Cd) exposure develops various serious pathological conditions because of its long-term retention in tissues. Withania somnifera (WS) is a known antioxidant due to its pharmacological properties, whereas vitamin E (VE) is a dietary antioxidant. The present study tries to study the combinational effect of WS and VE against Cd-induced toxicity.

Methods: Amelioration of Cd-induced toxicity by WS and VE was evaluated in mice liver by studying biochemical, morphological and genotoxic parameters. For this study mice (n=6) were given short-term Cd exposure of 5 mg/kg b. wt. intraperitoneally (ip) for 5 d. WS leaf extract was given orally (500 mg/kg b. wt.) and in combination with VE (100 mg/kg b. wt.) to study its preventive effects against Cd-induced oxidative stress-mediated histopathological and genotoxic alterations.

Results: Cd significantly enhanced lipid peroxidation (LPO (p<0.0001) and declined the levels of antioxidant viz. reduced glutathione (GSH (p ≤ 0.0001), glutathione-S-Transferase (GST (p ≤ 0.0001), superoxide dismutase (SOD (p ≤ 0.0001) and catalase (CAT) (p ≤ 0.0001) activity. Histopathological examination of Cd-treated mice liver exhibited marked structural damage. Metal generated oxidative stress and imbalance of pro-and antioxidants forms the basis for marked structural changes and genotoxicity. Individual treatments of WS and VE reduced the Cd-induced toxicity but when WS was given in combination with VE for the same period, levels of antioxidants were brought to near normal levels.

Conclusion: WS in combination with VE reduced Cd-induced oxidative burden and restored the levels of anti-oxidant enzymes resulting into improved histoarchitecture and reduced genotoxic insult.


Keywords


Cadmium, Withania Somnifera, Vitamin E, Liver, Oxidative stress

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About this article

Title

EVALUATING THE PROTECTIVE EFFICACY OF COMBINATION AND WITHANIA SOMNIFERA AND VITAMIN E AGAINST CADMIUM INDUCED OXIDATIVE STRESS MEDIATED HEPATIC HISTOPATHOLOGY AND GENOTOXICITY IN MURINE MODEL

Keywords

Cadmium, Withania Somnifera, Vitamin E, Liver, Oxidative stress

DOI

10.22159/ijpps.2017v9i10.20009

Date

02-10-2017

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Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol. 9, Issue 10, 2017 Page: 230-235

Online ISSN

0975-1491

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Authors & Affiliations

Seema Rani
Department of Zoology, Panjab University, Chandigarh, 160014, India
India

Vijay Lakshmi Sharma
Department of Zoology, Panjab University, Chandigarh, 160014, India
India

Mani Chopra
Department of Zoology, Panjab University, Chandigarh, 160014, India
India


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