EVALUATING THE PROTECTIVE EFFICACY OF COMBINATION AND WITHANIA SOMNIFERA AND VITAMIN E AGAINST CADMIUM INDUCED OXIDATIVE STRESS MEDIATED HEPATIC HISTOPATHOLOGY AND GENOTOXICITY IN MURINE MODEL
Objective: Cadmium (Cd) exposure develops various serious pathological conditions because of its long-term retention in tissues. Withania somnifera (WS) is a known antioxidant due to its pharmacological properties, whereas vitamin E (VE) is a dietary antioxidant. The present study tries to study the combinational effect of WS and VE against Cd-induced toxicity.
Methods: Amelioration of Cd-induced toxicity by WS and VE was evaluated in mice liver by studying biochemical, morphological and genotoxic parameters. For this study mice (n=6) were given short-term Cd exposure of 5 mg/kg b. wt. intraperitoneally (ip) for 5 d. WS leaf extract was given orally (500 mg/kg b. wt.) and in combination with VE (100 mg/kg b. wt.) to study its preventive effects against Cd-induced oxidative stress-mediated histopathological and genotoxic alterations.
Results: Cd significantly enhanced lipid peroxidation (LPO (p<0.0001) and declined the levels of antioxidant viz. reduced glutathione (GSH (p â‰¤ 0.0001), glutathione-S-Transferase (GST (p â‰¤ 0.0001), superoxide dismutase (SOD (p â‰¤ 0.0001) and catalase (CAT) (p â‰¤ 0.0001) activity. Histopathological examination of Cd-treated mice liver exhibited marked structural damage. Metal generated oxidative stress and imbalance of pro-and antioxidants forms the basis for marked structural changes and genotoxicity. Individual treatments of WS and VE reduced the Cd-induced toxicity but when WS was given in combination with VE for the same period, levels of antioxidants were brought to near normal levels.
Conclusion: WS in combination with VE reduced Cd-induced oxidative burden and restored the levels of anti-oxidant enzymes resulting into improved histoarchitecture and reduced genotoxic insult.
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