LARVICIDAL AND ANTIBACTERIAL EFFICACY OF GREEN SYNTHESISED SILVER NANOPARTICLES USING MELIA DUBIA
Objectives: Plant extracts seems to be a better alternative to control pathogenic organisms due to the presence of many bioactive compounds. In addition, the plant mediated synthesized silver nanoparticles also have important applications in the field of biology, the present study aims to study the activity of AgNPâ€™s (Silver Nanoparticles) synthesized using the leaves of Melia dubia against the 4th instar larvae of Culex quinquefasciatus and pathogenic microbes
Methods: The synthesized AgNPâ€™s were characterized by UV-Vis spectrum, Scanning Electron Microscopy (SEM), Energy dispersive X-ray spectroscopy(EDX) and Fourier Transform Infrared Spectroscopy (FTIR). The 4th instar larvae of Culex quinquefasciatus were exposed to different concentrations of aqueous leaf extract (5, 10, 15, 20, 25ppm) and Silver nanoparticles (1, 2, 3, 4, 5 ppm) for 24 hours period. The antibacterial activity was measured by disk diffusion method against Bacillus subtilis, Proteus mirabilis, Klebseilla pneumoniae, Escherichia coli, and Vibrio cholerae.
Results: The observations reveal that the silver nanoparticles synthesized using Melia dubia leaves were more effective than the crude leaf extract of the plant over Culex quinquefasciatus and microbes tested. Moreover, the results of metabolites also revealed that the AgNPâ€™s significantly altered the carbohydrates and protein level in the 4th instar larvae of Culex quinquefasciatus.
Conclusion: The larvicidal and bactericidal activities are due to various phytoconstituents coated with the nanoparticles. It is further inferred from the results that the use of AgNPâ€™s for the control of targeted vectors is to eradicate disease transmitting mosquitoes as well as to control microbes.
Keywords: Melia dubia, Silver Nanoparticles, FTIR, SEM, EDX, Antibacterial activity, Larvicidal activity.Â
2. Lara HH, Ayala-NuÃ±ez NV, Ixtepan-Turrent L, Rodriguez-Padilla C. Bactericidal effect of silver nanoparticles against multidrug-resistant bacteria. World J Microbiol Biotechnol 2010;26:615-21.
3. Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK. Antimicrobial effects of silver nanoparticles. Nanomedicine:nanotechnology. J Biology and Medicine 2007;3(1):95-101.
4. Ahmad T, Wani IA, Manzoor N, Ahmed J, Asiri AM. Biosynthesis, structural characterization and antimicrobial activity of gold and silver nanoparticles. J Colloids Surf B Biointerfaces 2013;107(1):227-34.
5. Karthikeyan J, Srinivasan PT, Krithika RS, Bavani G. Extracellular synthesis of silver nanoparticles by Menta Piperita (Leaf Broth) and Bacillus subtilis (Culture Supernatant):a comparative study. Int J Nanotechnol 2011;5(1Appl):37-45.
6. World Health Organization Lymphatic filariasis Fact sheet No.102 2012.
7. Jantan L, YalvemaMF, Ahmed NW, Jamal JA. Insecticidal activities of the leaf oils of eight Cinnamomum species against Aedes aegypti and Aedes albopictus. J Pharm Biol 2005;43:526-32.
8. Yang YC, Lee SG, Lee HK, Kim Mk, Lee SH, Lee HS. A piperidine amide extracted from Piper longum L. fruit shows activity against Aedes aegypti mosquito larvae. J Agri Food Chem 2002;50:3765-67.
9. Cheng SS, Liu JY, Tsai KH, Chen WJ, Chang ST. Chemical composition and mosquito Larvicidal activity of essential oils from leaves of different Cinnomomum osmophloeum provenances. J Agric Food Chem 2004;52:4395-400
10. Govindarajan M. Larvicidal efficacy of Ficus benghalensis L. plant leaf extracts against Culex quinquefasciatus Say, Ades aegyptiL and Anopheles stephensi L.(Diptera:Culicidae). J Eur Rev Med Pharmacol Sci 2010;14:107-11.
11. Savithramma M, Linga Rao M, Rukmini K, Suvarnalatha Devi P. Antimicrobial activity of Silver nanoparticles synthesized by using Medicinal Plants. Int J Chem Tech Res 2011;3:1394-1402.
12. Nasrollahi A, Pourshamsian Kh, Mansourkiaee P. Antifungal activity of silver nanoparticles of some fungi. Int J Nano Dim 2011;1(3):233-9.
13. Suprapti S, Djarwanto, Hudiansyah. The resistance of five wood species against several wood destroying fungi. J Peneli Hasil Hutan 2004;22(4):239-46.
14. Kiritkar KR Basu BD. Indian Medicinal Plants (eds Blatter, E., Caicus,J.F. and Mhaskar,K.S.) International Book Distributers, Dehradun. Vol.1, pp 545-46. 1999.
15. Sharma IM, Bhardwaj SS. Use of plant extracts and yeast antagonists in the management of storage scab and rots of apple fruits. J Biol Control 2000;14:17-23.
16. Koul O, Multani JS, Singh G, Wahab S. Bioefficacy of toosendanin from Melia dubia (syn.M.azedarach) against gram pod borer, Helicoverpa armigera (Hubne). Current Sci (India) 2002;83:1387-91.
17. Reuben R, Tewari SC, Hiriyan J, Akiyama J. Illustrated keys to species of Culex (Culex) associated with Japanese encephalitis in Southeast Asia (Diptera:Culicidae). J Mosquito Systematics 1994;26 (2):75-96.
18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein Measurement with the. J Folin Phenol Reagent 1951;193(1):265-75.
19. Du Bois M, Gilles K, Hamilton J, Rebers P, Smith F. Colorimetric method for determination of sugars and related substances. J Analytical Chemistry 1956;28(3):350-6.
20. Hewitt W, Vincent S. Assay design and evaluation. In Theory and Applications of Microbiological Assay. 1st edn. (Hewitt,W &Vincent,S., Eds) p. 185-211. San Diego Academic Press, 1989.
21. Raut RW, Jaya RL, Niranjan K, Vijay DM, Sahebrao BK. Photosynthesis of Silver Nanoparticles Using Gliricidia sepium (Jacq.). J Current Nanoscience 2009;5:117-22.
22. Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS. â€œCinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity.â€ J Colloids and Surfaces B 2009;73:332-8.
23. Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, Wang H, Wang Y, Shao W, He N, Hong J, Chen C. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. J Nanotechnology 2007;18:104-105.
24. Mukherjee P, Roy M, Mandal BP. Green synthesis of highly stabilized nanocrystaline silver particles by a non pathogenic and agriculturally important fungus T. asperellum. J Nanotechnology 2008;19:075103.
25. Nath BS, Suresh A, Varma BM, Kumar RPS. Changes in protein metabolism in hemolymph and fat body of the silkworm, Bombyx mori (Lepidoptera:Bombycidae) in response to organophosphorus insecticides toxicity. J Ecotoxicol Environ Safety 1997;36 (2):169-73.
26. Sharma P, Mohan L, Dua KK, Srivastava CN. Status of carbohydrate, protein and lipid profile in the mosquito larvae treated with certain phytoextracts. Asian Pacific J Tropical Medicine 2011;301-4.
27. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. J Biotechnol Adv 2009;27:76-83.
28. Sondi I, Sondi SB. Silver nanoparticles as antimicrobial agent:a case study on E.coli as a model for Gram â€“negative bacteria. J Colloid Interface Sci 2004;275:177-82.
29. Halt KB, Bard AJ. Interaction of silver (I) ions with the respiratory Chain of Escherichia coli:an electrochemical and scanning electrochemical microscopy study of the antimicrobial mechanism of micromolar Ag+. J Biochemistry 2005;44 (39):13214-23.
30. Warisnoicharoen W, Hongpiticharoen P, Lawanprasert S. Alteration in Enzymatic function of Human cytochrome P450 by silver nanoparticles. Res J Environ Toxicol 2001;5:58-64.
31. Hatchett DW, Henry S. Electrochemistry of sulfur adlayers on the low-index faces of silver. J Phys Chem 1996;100:9854-59.