Int J Pharm Pharm Sci, Vol 9, Issue 10, 7-12Original Article



1Endocrinology and Reproductive Physiology Laboratory, Department of Physiology, University of Calcutta, 92, APC Road, Kolkata 700009, 2Department of Zoology (PG Section), D. M. College of Science, Imphal 795001

Received: 29 May 2017 Revised and Accepted: 31 Aug 2017


Objective: Natural goitrogens present in plant foods or antithyroidal drugs both alter the morphology and normal functional status of the thyroid gland interfering the synthesis and secretion of thyroid hormones. In this study, the goitrogenic potential of bamboo shoots (BS) containing natural goitrogens has been compared against a potent syntheticantithyroidal drug thiourea.

Methods: BS was fed to 6 rats by 1/3rdreplacement of of 180grams (g) of their food i.e.60g of BS containing 35g of goitrogens of cyanogenic originsuch that each rat ideally consumed 6 mg/100g of body weight per dayandthiourea at a near equal dose of 6 mg/100g of body weight per day was force fed to another 6 rats for a period of 45 d. Changes in food consumption pattern, serum toxicity marker SGOT and SGPT, morphological changes like thyroid gland weight and histology and functional aspects like TPO activity, Na+-K+ATPase activity, urinary iodine excretion and serum T3,T4 and TSH levels induced by this natural and synthetic agent respectively were investigated. Results analyzed by ANOVA followed by post hoc tests.

Results:Food consumption pattern was similar in all groups while serum toxicity markers SGOT,SGPT showed anon-significant increase in experimental groups. Increase in body and thyroid gland weight as compared to control was seen on treatment with both antithyroidal agents, agreater percentage of increase was seen in thiourea treated group. TPO activity, Na+-K+ATPase activity, serum T3 and T4levels were decreased significantly in both treated groups as compared to normal, with maximum inhibition in the thiourea treated group. Histological plates of BS treated thyroid revealed hypertrophy and thyroid follicular cell disruption with microcytic infiltration. Thiourea treated gland histological plate revealed complete abolition of normal histoarchitecture with marked changes in colloidal area and hyperplasia with folding and papillae on theepithelium.

Conclusion: In overall, the data indicatethatthough the goitrogenic potentiality of BS cannot be considered having similar efficacy to that of thiourea but as a food entity by itself, it has potent antithyroidal activity even in presence of adequate iodine intake.

Keywords:Dietarygoitrogen, Thyroid, Thiourea, Bamboo-shoots, Thyroid peroxidase


Thiourea is an establishedantithyroid agent whose derivatives form commercially available drugs like methimazole, carbamizole and propylthiouracil (PTU), etc [1]. Several other derivatives including ethylenethiourea (ETU) occur in fungicides, cigarette smoke and as primary work reagents in rubber industries [2]. Thiourea causes disruption of peroxidase activity in the thyroid gland leading to inhibition of thyroid hormone production and in animal testing has produced thyroid hyperplasia, decreased serum thyroxine levels and increased thyroid stimulating hormone (TSH) level [3]. However, prolonged exposure has reportedly resulted in thyroid follicular cell adenomas, carcinomas, liver and pituitary tumours. Several other side effects like gastrointestinal disorders, blood corpuscles disorders, raised thetemperature and reproductive disorders have been associated with usage of such drugs [4].

Bamboo-Shoots (BS) in contrast to the synthetic drug thiourea is a natural goitrogenic cruciferous vegetable which is consumed almost as a staple food in the eastern regions of the world and causes endemic goitre in the North Eastern regions of India, where it is consumed mostly on a regular basis [5]. Its chemical analysis has revealed it to contain several anti-thyroidal constituents like cyanogenic glycosides, glucosinolatesandthiocyanate around 600 mg/kg wet weight on the whole [6]. Previous studies have also revealed its ability to inhibit peroxidase activity of thyroid, however, its exact mode of action is yet to be described [7]. Divergent to the side effects of thiourea, BS is actually gaining popularity for its many health benefits comprising improvement of cholesterol risks, cardiovascular disorders and anti-carcinogenic effects..

Although separately known for their antithyroid effects, a comparative analysis of the goitrogenic potential of such a natural goitrogen against an established antithyroid drug is not available. Rationality of this study is to establish the antithyroidal potentiality of BS in contrast to thiourea, reinforcing a comparative analysis of their thyroid disrupting potentiality.


Experimental design

For theinvestigation, eighteen adult female rats (six as thiourea fed animals, six as BS fed animals and six as control animals) of 90±10 g body weight were used. The animals were obtained from M/S Chakraborty Enterprise, Kolkata, registered breeder vide Registration Number 1443/PO/b/11/CPCSEA and maintained as per national guidelines and protocols as approved by the Institutional Animal Ethics Committee (IAEC) number-IAEC/PG Project/Endo Special/2014. They were housed in three cages having six each; in air-conditioned rooms maintaining twelve hours light/twelve hours dark cycles. The rats had free access to drinking water and were fed on standardized normal laboratory diet (20% protein) which consisted of 70% wheat, 20% Bengal gram, 5% fish meal powder, 4% dry yeast powder, 0.75% refined tiloil and 0.25% shark liver oil [8]. BS was fed to 6 rats by 1/3rd replacement of 180g of their food, such that 60 g of BS containing 35 mg of goitrogens of cyanogenic origin (cyanogenicglucosides, glucosinolates and thiocyanate) was available at an ideal amount of 6 mg/100 g of body weight per rat and thiourea at a near equal dose of the antithyroid constituents of BS at 6 mg/100g of body weight was force-fed to another 6 rats for a period of 45 d. This is much below the oral LD50 for thiourea at 12 mg/100g [9] and lies in the range of daily maintenance dose of hyperthyroid drug intake of 5 mg-15 mg per day [10].

Chemicals and reagents

Chloroform, Potassium Iodide (KI), Hydrogen Peroxide (H2O2), Sodium Chloride (NaCl), Potassium Chloride (KCL), Sodium Dodecyl Sulphate (SDS) were purchased from Sisco Research Laboratories (SRL), Mumbai, India; bovine serum albumin (BSA), Magnesium Chloride (MnCl2),Tris-ATP,ouabain were purchased from Sigma Chemical Company. T4 and T3 kits were purchased from RFCL Limited, India. TSH kits were purchased from Cusabio Biotech Limited. SGOT and SGPT kits were purchased from BBI solution (Cardiff, UK).

Preparation of bamboo shoot containing food

The experimental group of rats was fed bamboo shoots by replacing a1/3rd portion of thenormal diet. Sixty grams of bamboo shoots was weighed, equal amount of water was added to it and boiled for 15 min. It was then mixed with 180 grams of normal food as mentioned [8].

Food consumption pattern

Food consumption of each rat per day was recorded daily during the period of treatment.

Calculation of food consumption for each rat (g/rat/day) was calculated as:

Food given(g)−Food wasted(g)=food consumption(g)

Sacrifice of the animal, body and thyroid weight

The animals were sacrificed on the consecutive day of the last treatment following Institutional Animal Ethics protocol of the Department of Physiology, University of Calcutta. Blood was collected from the hepatic portal vein of each animal and stored separately. Before sacrifice, the body weight of each rat was taken. The thyroid gland from each animal was collected, weighed and stored separately.

Estimation of SGOT (Serum glutamic-oxaloacetic transaminase) and SGPT (Serum glutamic pyruvic transaminase)activities

The enzymatic kit of BBI solution (Cardiff, UK) was used to estimate SGOT and SGPT activities following the manufacturer’s protocol.

Measurement of thyroid peroxidase (TPO) activity

TPO was measured following the method of Alexander [11]. Thyroid glands from sacrificed animals were pooled (about 5 mg) and homogenized in a homogenizing tube containing 3-5 ml phosphate buffer (pH-7.4, 5 mmol). The mixture was homogenized for 30-45 secs at 4 °C in glass homogenizer of Potter-Elvehjem, Germany. The homogenate was transferred and centrifuged at 4 °C at 1000 rpm for 10 min to separate cell debris and obtain mitochondrial fraction. The supernatant was collected and utilized to measure the TPO activity. Peroxidase activity was determined using potassium iodide substrate (KI). The reaction mixture (2 ml) contained 50 mmol/lit KI, 0.33 mmol/lit H2O2 and approximately 100 µg of protein. H2O2solution was added last to start the reaction and the initial increase in absorbance at 353 nm was recorded in Shimadzu spectro-photometer. The protein level of thyroid tissue was determined by the method of Lowry [12] using bovine serum albumin as a standard. The peroxidase activities obtained were expressed as change in optical density (ΔOD)/min/mg protein).

Measurement of Na+-K+ATPase activity

Na+-K+ATPase Activity was measured by a modification of the Esmann method [13]. The microsomal fraction of the thyroid gland homogenate was incubated in reaction mixtures of (a) 30 mmol imidazole HCL, 130 mmolNaCl, 20 mmolKCl, 4 mmolMgCl2 and (b) 1 Mm ouabain at pH 7.4 for 60 min at 0 °C. The reaction was initiated by adding 4 mmol Tris-ATP at 37 °C and was stopped with theaddition of 20% SDS. Inorganic phosphate (Pi) thus liberated was determined by reading the absorbance at 850 nm in UVmini 1240, Shimadzu spectrophotometer, Japan by the method of Baginski et al. [14]. The enzyme activity was expressed in n moles of Pi liberated per hour per mg of protein from astandard curve of potassium dihydrogen phosphate.

Protein estimation

The protein content of samples was estimated following the method of Lowry et al. [12], using Bovine Serum Albumin (BSA) as standard.

Measurement of serum thyroxine (T4) and triiodothyronine (T3) and thyroid stimulating hormone (TSH)

The serum obtained from the experimental and control group of animals were used to measure T4 and T3 by Enzyme-Linked Immunoabsorbent assay (ELISA) kits obtained from RFCL Limited, India as per manufacturer’s instructions.

TSH was measured via the same principle using kits obtained from Cusabio Biotech Limited, Rat TSH Kit following manufacturer’s protocol.

Urinary iodine estimation

The animals were placed in metabolic cages and the collected urine samples of the animals were washed in a muffle furnace at 600 °C after digestion in strong alkali. Iodide was then assayed maintaininginternalqualitycontrolby its catalytic action on the reduction ofceric ion (Ce2+) to thecerous ion (Ce3+) as per the method of Karmakar et al.[15].

Histological study

0n the day of the sacrifice, the thyroid glands of each group of animals were removed, weighed and fixed in formal (4%) solution. The tissues were dehydrated by placing in gradually higher gradations of alcohol and embedded into paraffin to make blocks. Thyroid sections were cut using a microtome and collected on a slide. Sections were stained with haematoxylin and eosin and observed under a light microscope.

Statistical analysis

Results obtained were presented as mean±standard deviation. The differences if any between the groups wereanalyzed using Analysis of Variance (ANOVA) followed by Tukey’s test. The level of significance was tested at P<0.05. Statistical calculations were performed using Vassarstats online and MS Excel Office 2010 [16].


Body weight

Maximumpercent gain of body weight was observed in thiourea treated group, while percent value of Bamboo-Shoots fed group also remained higher than thecontrol group (table 1).

Food consumption pattern

Food consumption pattern was noted in each group for the entire period study and it was observed that feeding amounts remained almost similar in both experimental groups in view of thecontrol group, with a non-significant decrease in the BS fed group as compared to control group (table 2).

Table 1: Changes in body weight of experimental after exposure to thiourea and bamboo-shoots respectively

Group Initial body weight(g) Gain of body weight (%) Final body weight (g)
Control 76.25±3.97 (35.08) 103±4.12
Thiourea treated 74.44±3.02 (59.08)a 118.42±4.23
Bamboo-Shoot fed 75.87±3.57 (45.24)c,b 110.20±0.08

Data is represented as mean±SD (standard deviation), n=6, Values significantly different by ANOVA (Analysis of Variance) (P<0.05) are denoted by superscripts. a Control versus thiourea, b Control versus Bamboo-Shoots (BS) fed and c thiourea versus Bamboo-Shoots (BS) fed.

Table 2: Changes in food consumption pattern after exposure to thiourea and bamboo-shoots respectively

Parameters Groups
Control Thiourea treated Bamboo-shoots fed
Food intake/day/rat (g) 25±1.6 25±2.1 24±1.3

Data is represented as mean±SD, n=6, No significant difference found.

SGOT and SGPT activities

There was a minor increase in SGOT and SGPT levels in both experimental groups as compared to control, while greater but non-significant change occurred in thiourea administered animals (table 3)

Thyroid gland weight

The weight of the thyroid gland increased significantly (P<0.05) in both the goitrogen treated groups in comparison to control group with themaximum increase being noted in thiourea treated group. Thyroid gland weight of thiourea treated group was significantly greater than that of BS fed group (fig. 1)

Table 3: Changes in SGOT and SGPT activities after exposure to thiourea and bamboo-shoots respectively

Parameters Control Thiourea treated Bamboo-shoots fed
SGOT 21.93±0.13 22.58±0.18 22.45±0.22
SGPT 27.63±0.23 28.72±0.31 28.26±0.21

Data is represented as mean±SD, n=6, Values significantly different by ANOVA (P<0.05) are denoted by superscripts.

Fig.1: Changes in thyroid weight after exposure to thiourea and bamboo-shoots respectively

Data is represented as mean±SD (standard deviation), n=6, Values significantly different by ANOVA (Analysis of Variance) (P<0.05) followed by post-hoc tests are denoted by superscripts, a Control versus thiourea, b Control versus Bamboo-Shoots (BS) fed and c thiourea versus Bamboo-Shoots(BS)fed.

TPO activity

A significant decrease (P<0.05) in TPO activity was observed in both experimental groups in view of thecontrol group. If thedecrease in TPO activity of thiourea treated group is considered 100% then the relative decrease in TPO activity of BS fed animals was 73.10% of thiourea treated group. (fig. 2)

Fig.2: Changes in thyroid peroxidase (TPO) activityafter exposure to thiourea and bamboo-shoots respectively

Data is represented as mean±SD (standard deviation), n=6, Values significantly different by ANOVA (Analysis of Variance) (P<0.05) followed by post-hoc tests are denoted by superscripts, a Control versus thiourea, b Control versus Bamboo-Shoots (BS)fed and c thiourea versus Bamboo-Shoots (BS)fed.

Na+-K+ATPase activity

Compared to control group Na+-K+ATPase activity was diminished significantly (P<0.05) in both thiourea treated and BS fed thegroup. However, decrease in BS fed group was 67.26% in comparison of considered 100% decrease of thiourea treated group (fig. 3).

Fig.3: Changes in Na-K ATPase activityafter exposure to thiourea and bamboo-shoots respectively

Data is represented as mean±SD (standard deviation), n=6, Values significantly different by ANOVA (Analysis of Variance) (P<0.05) followed by post-hoc tests are denoted by superscripts, a Control versus thiourea, b Control versus Bamboo-Shoots(BS) fed and c thiourea versus Bamboo-Shoots(BS) fed.

Thyroid hormone levels

Thyroid hormone levels were significantly low in both test groups in comparison to control. T3 levels decreased 41.36% in BS fed group relative to 100% decrease in thiourea treated group while T4 levels in BS fed group decreased to 53.80% of thiourea treated group levels (fig. 4).

TSH levels increased significantly in both experimental groups, with thehighest upsurge in thiourea treated group. Considering the rise in thiourea treated group to be 100%, the increase in BS fed group was 76% (fig. 5)

Fig.4: Changes in triodothyronine (T3) and Thyroxine (T4) levelsafter exposure to thiourea and bamboo-shoots respectively

Data is represented as mean±SD (standard deviation), n=6, Values significantly different by ANOVA (Analysis of Variance) (P<0.05) followed by post-hoc tests are denoted by superscripts, a Control versus thiourea, b Control versus Bamboo-Shoots (BS)fed and c thiourea versus Bamboo-Shoots (BS) fed.

Fig.5: Changes in thyroid stimulating hormone (TSH) levels after exposure to thiourea and bamboo-shoots respectively

Data is represented as mean±SD (standard deviation), n=6, Values significantly different by ANOVA (Analysis of Variance) (P<0.05) followed by post-hoc tests are denoted by superscripts, a Control versus thiourea, b Control versus Bamboo-Shoots (BS)fed and c thiourea versus Bamboo-Shoots (BS)fed.

Urinary Iodine levels

Urinary iodine levels showed arise in both investigational groups in assessment, with thegreatest significant increase observed in thiourea treated group (fig. 6).

Fig.6: Changes in urinary iodine levels after exposure to thiourea and bamboo-shoots respectively

Data is represented as mean±SD (standard deviation), n=6, Values significantly different by ANOVA (Analysis of Variance) (P<0.05) followed by post-hoc tests are denoted by superscripts, a Control versus thiourea, b Control versus Bamboo-Shoots (BS) fed and c thiourea versus Bamboo-Shoots (BS) fed.

Fig.7: Photomicrographs of HandE stained thyroid sections (400x) A) Control, B), Thiourea treated, C) Bamboo-Shoots fed, C = Colloid, F = Follicle

Histological studies of thyroid

Normal thyroid histology showed even epithelium and regularly shaped cells filled with colloid. Histological comparison of BS fed animal’s thyroid to normal revealed general thyroid follicular cell disruption and microcytic infiltration. Follicular size increased markedly resulting in rupture of follicles and evacuation of follicular colloid materials indicating a discernable hypertrophy of gland. Thiourea treated animal’s glands exhibit complete abolition of normal histoarchitecture with definite changes in the colloidal area. Congestion of follicle due to increase in number, resorption of colloidal materials, hyperplasia with huge folding and papillae on epithelium were other distinct features seen (fig. 7).


Even with the advent and prevalent usage of anti-thyroidal drugs since a protracted amount of time, hyperthyroidism and other related disorders remain a common problem worldwide with a greater proportion of negative impacts of such drugs coming to light. Presently, however, several natural food products emerging as nutraceuticals are being used worldwide to treat thyroid disorders. Although several vegetables of the brassicae family like radish, cabbage, brussel sprouts, turnips, mustard and rapeseed, etc are known for their goitrogenic activity [17], a broad database of comparative goitrogenic potential between commercially available known anti-thyroidal drugs used to treat hyperthyroidism and having other environmental exposure with such natural goitrogens has not been compiled. This study has thus been designed simplistically to compare the goitrogenic potentiality of such a natural goitrogen bamboo-shoots, which on daily consumption reportedly causes endemic goiter in Manipur, India [5] and a well-knownchemical anti-thyroidal agent thiourea which is the major constituent of several synthetic drugs used in treatment of hyperthyroidism like methimazole, PTU [18], etc

BS was fed in the amount such that the total amount of reported antithyroidal components in it was approximately equivalent to a thiourea dose of 6 mg/100 g of body weight. Food consumption pattern for both experimental groups remained nearly similar to control animals indicating almost comparable intake of both antithyroidalexposures.

Serum SGOT and SGPT values did not vary significantly between the control and experimental groups representing theabsence of toxicity occurrence [19]. However, values for thiourea treated animals remained elevated as compared to BS fed animals expressing manifestation of a greater harmful outcome.

To conform with the well-known reality that there is increase in body weight in hypothyroidism [20], both groups of experimental animals exposed to anti-thyroidal treatment namely thiourea and BS feeding exhibited a percent gain in body weight as compared to the normal animals, however, a greater percent increase was seen in thiourea treated group leading to the conclusion that probably the degree of hypothyroid induction was much greater by thiourea in comparison to BS. In absolute association to this, the maximum amount of significant gain in thyroid weight was also noted in thiourea treated animals, followed by BS fed animals in view of the control animals. Previous studies with both thiourea [21] and other natural goitrogens similar to BS like cassava [22] have yielded similar results individually; however, a comparison was done only in this study, indicative of the greater antithyroidal capacity of thiourea.

Histological plates of the thyroid glands of both the treated groups revealed prominent hypertrophic and hyperplastic changes, while there was much greater histoarchitecturedisruption and extreme follicle congestion along with rupture and papillae on the epithelium in the thiourea treated animals. Microcystic infiltration was also noted in the BS fed group. At a glance, however, it is very apparent that the disruptive changes are much greater in the thiourea treated group, indicative of themuch greater efficacy of the synthetic drug against the natural goitrogen. Similar disruptive changes have been found in ethylene thiourea fed rats [23] and also in another natural goitrogen like cassava, radish fed animals [24].

The aberrations in the thyroid morphology caused due to the exposure to the goitrogens contributed to the significant decrease in T3 and T4, in addition, a complementary increase in TSH level in both treated groups as equated with the control. T3 and T4 levels decreased near to 50% and TSH increased to 76% in BS fed animals in comparison of levels inthiourea fed animals. T3 and T4 lie in direct feedback control of TSH via the pituitary-thyroid axis and affect each other inversely [25]. As previously mentioned, the enlargement of the gland can be expected to be in direct connection with the hypersecretion of TSH leading to an underactive thyroid.

Suppressed thyroid hormone levels were indicative of a decrease in their synthesizing enzyme which was validated by testing the levels of thyroid peroxidase (TPO) activity of all groups. TPO plays a pivotal role in T3 and T4 synthesis being the primary enzyme in association with thyroglobulin in iodideorganification and coupling [26]. Both experimental groups confirmed to a decline in TPO activity in analysis with the control group, nonetheless, thiourea treated animals in concordance with all previous parameters revealed the greatest decrease in TPO activity. Considering this decrease to be 100% in relation to the control, the decrease in BS fed animals was 73.10%. Thiourea and its derivatives are known to cause TPO inhibition by interfering with TPO-catalyzed iodine formation with related oxidative metabolism. Synthetic drugs reportedly can act on peroxidase and/or the molecular iodine which maybe produced by oxidation of iodides (2I-—»I2 —» 2I+)to cause disruption [27]. Previous studies in Bambusaarundinacea subtype of BS have also been reported to interfere with TPO activity in vivo, although the exact mechanism remains unidentified [8].

Na+-K+ATPase is located in the basolateral membrane of the thyroid follicular cells and in association with sodium iodide symporter (NIS) is responsible for follicular iodide uptake and transport [28]. In this study, Na+-K+ATPase activity decreased significantly in both investigational groups relative to the control group, thegreater decrease was seen in thiourea treated group. In comparison to thiourea treated group, the decrease in BS fed group was 67.26%. Thiourea along with its spin-off products like tetramethylthiourea, ethylenethioureaare well–acknowledged inhibitors of iodide uptake via NIS, similarly, in theassociation, a complementary decrease in Na+-K+ATPase activity can be predicted, a likely explanation for suppression of Na+-K+ATPase activity by the goitrogen exposures in this study [29]. Earlier studies with other thiocyanate exposure have reported a decrease in Na+-K+ATPase activity, explaining the decrease in BS fed group as BS releases thiocyanate on breakdown [30].

Urinary iodine level is asuggestive marker of iodine nutritional status as a high percent (over 90) of iodine consumed is excreted in the urine. Greater urinary iodine concentration reflects more iodine efflux and less utilization in the gland [31]. Thiourea competitively inhibits iodine uptake by severely reducing the activity of NIS and causing iodine complexation during iodide oxidation [29]. On the other hand, the antithyroidal constituents in BS like cyanogenic glycosides, glucosinolates break down to form thiocyanate, in addition to the basic thiocyanate content of the vegetable [8]. Thiocyanate is a well-established inhibitor of iodide transporter in the thyroid but is more potent in increasing iodide efflux which is reflected in increased urinary iodine levels of consumption of such vegetables [32]. Similarly, in this study, increased urinary iodine levels were found in both experimental groups, but a greater enhancement was seen in thiourea treated group elucidating that less iodine was utilized and up taken in this group leading to decrease in T3 and T4 synthesis in turn. Thiocyanate from the BS also inhibited iodine uptaken but to a much lesser extent in comparison to thiourea.


Thiourea in comparison to BS has much severe down regulatory activities in the thyroid gland, at all stages of hormone synthesis that include iodide uptake, iodide organification and coupling of iodotyrosine molecules by decreasing the activities of concerned enzymes resulting marked alteration thyroid morphology as well as physiology. However, BS has the potentiality to cause significant inhibition on those regulatory elements but with relatively less severity and toxicity. The overall results reveal that the efficacy of BS in suppressing thyroid activities is not less than a proximate average of 50% of thiourea. In retrospection it can be thus proposed that the antithyroidal components in BS have the potentiality to cause adiminution of thyroid activity but with less toxic implications that in turn can be formulated into antithyroidal drugs of natural origin in long run with lessside effects.Further studies are obligatory to bring about thecomparison of the molecular mechanism and potentially negative implications of the two materials which are ongoing in the investigator’s laboratory.


T3-Triiodothyronine, T4-Thyroxine, TSH-Thyroid Stimulating Hormone, SGOT-Serum glutamic-oxaloacetic transaminase), SGPT-Serum glutamic pyruvic transaminase, BS-Bamboo Shoots, SD-Standard Deviation, ANOVA-Analysis of Variance, TPO-Thyroid Peroxidase, PTU-Propylthiouracil.


Authors are thankful to BI (92) research grant of Department of Physiology, University of Calcutta. DeotimaSarkar gratefully acknowledges financial assistance from DST INSPIRE Fellowship Scheme, Govt. of India.


DeotimaSarkar:Data collection and analysis, drafting article, writing manuscript

ArijitChakraborty:Data collection and analysis, critical revision of the article

Chandrima Bhattacharya:Data collection

Laishram Hemchandra Singh: Sample and data collection

Prof. Amar K Chandra:Design of the work, data analysis, critical revision of the article, final approval of theversion to be published.


The authors declared no potential conflicts of interest with respect to the authorship, research, or publication of this article.


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  • Deotima Sarkar, Arijit Chakraborty, Chandrima Bhattacharya, Laishram Hemchandra Singh, Amar K Chandra. Exploration of thegoitrogenic/antithyroidal potentiality of bamboo-shoots in relation to thiourea.Int J Pharm Pharm Sci 2017;9(10):7-12.

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Dietarygoitrogen, Thyroid, Thiourea, Bamboo-shoots, Thyroid peroxidise





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International Journal of Pharmacy and Pharmaceutical Sciences
Vol. 9, Issue 10, 2017 Page: 7-12

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Authors & Affiliations

Deotima Sarkar
Endocrinology and Reproductive Physiology Laboratory, Department of Physiology, University of Calcutta, 92, APC Road, Kolkata 700009

Arijit Chakraborty
Endocrinology and Reproductive Physiology Laboratory, Department of Physiology, University of Calcutta, 92, APC Road, Kolkata 700009

Chandrima Bhattacharya
Endocrinology and Reproductive Physiology Laboratory, Department of Physiology, University of Calcutta, 92, APC Road, Kolkata 700009

Laishram Hemchandra Singh
Department of Zoology (PG Section), D.M. College of Science, Imphal 795001

Amar K. Chandra
Endocrinology and Reproductive Physiology Laboratory, Department of Physiology, University of Calcutta, 92, APC Road, Kolkata 700009

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