SPECTRAL STUDIES OF THE INTERACTION BETWEEN SOLIFENACIN SUCCINATE AND HERRING SPERM DNA
Objective: The interaction between solifenacin succinate (SFS) and herring sperm DNA (Hs-DNA) was investigated by using UVâ€“vis and fluorescence spectra at pH 7.4.
Methods: The UV-vis and fluorimetric titrations were performed by keeping the concentration of SFS constant and varying the concentration of Hs-DNA in a buffer solution of pH 7.4. The absorption spectra shows an increase in absorbance at an absorption band of SFS with increasing concentration of Hs-DNA. A quenching of intrinsic fluorescence of SFS was observed with the increasing of Hs-DNA concentration.
Results: The fluorescence lifetime results, the varieties of the fluorophore absorption spectra and the decrease of the binding constant with the increasing temperature all indicate that the fluorescence quenching is static. The ratio and constant of the binding Hs-DNA to SFS are 1 and 6.76 x 105, respectively. The values of Î”H, Î”S and Î”G obtained for SFS-Hs-DNA were - 9.93 kJ/mol, 0.17 kJ/(mol K) and - 44.78 kJ/mol respectively at 298 K.
Conclusions: The absorption spectra and fluorescence quenching spectra of SFS in presence of Hs-DNA strongly supports the interaction of SFS with Hs-DNA. SFS binds to Hs-DNA with a binding constant of 6.76 x 105 at 298 K. In addition, thermodynamic data for the SFS binding to Hs-DNA showed that the binding of SFS to Hs-DNA is not only exothermic but entropy-driven.
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